Molecular and immunohistochemical characteristics of complete hydatidiform moles
Molar pregnancy is a gestational trophoblastic disease that belongs to the category of precancerous lesions. On the other end of the spectrum are gestational trophoblastic neoplasms such as invasive mole, choriocarcinoma, placental site trophoblastic tumor and epithelioid trophoblastic tumor, which...
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| Format: | Article |
| Language: | English |
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Sciendo
2017-06-01
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| Series: | Balkan Journal of Medical Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/bjmg-2017-0009 |
| _version_ | 1797688865008386048 |
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| author | Kubelka-Sabit KB Prodanova I Jasar D Bozinovski G Filipovski V Drakulevski S Plaseska-Karanfilska D |
| author_facet | Kubelka-Sabit KB Prodanova I Jasar D Bozinovski G Filipovski V Drakulevski S Plaseska-Karanfilska D |
| author_sort | Kubelka-Sabit KB |
| collection | DOAJ |
| description | Molar pregnancy is a gestational trophoblastic disease that belongs to the category of precancerous lesions. On the other end of the spectrum are gestational trophoblastic neoplasms such as invasive mole, choriocarcinoma, placental site trophoblastic tumor and epithelioid trophoblastic tumor, which are considered malignant tumors. Based on defined histopathological criteria, molar pregnancy is divided into partial and complete hydatidiform mole. Especially in the case of early complete mole, the diagnosis can be quite challenging and often necessitates additional molecular or immunohistochemical methods. The aim of this study was to assess the importance of additional molecular and immunohistochemical methods to accurately diagnose complete hydatidiform mole and to stress the importance of correct diagnosis and close follow-up of these patients. A total of 367 consecutive cases of spontaneous abortion were analyzed in a 3-year period. Eight cases with histopathological diagnosis of complete molar pregnancy were selected for further analysis. Apart from standard microscopic analysis, additional molecular and immunohistochemical analyses were performed in all eight cases. Most of the histopathological characteristics of complete molar pregnancy were present in all cases, together with complete absence of positivity for the p57 immunohistochemical marker in the cytotrophoblasts and villous stromal cells. The molecular analysis revealed androgenetic diploidy in seven cases and biparental diploidy in one case with more than three consecutive complete molar pregnancies. Additional immunohistochemical and molecular methods can considerably aid in the correct diagnosis of molar pregnancy. |
| first_indexed | 2024-03-12T01:36:47Z |
| format | Article |
| id | doaj.art-ff57fa5f13c24a3cafda059f08264295 |
| institution | Directory Open Access Journal |
| issn | 1311-0160 |
| language | English |
| last_indexed | 2024-03-12T01:36:47Z |
| publishDate | 2017-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Balkan Journal of Medical Genetics |
| spelling | doaj.art-ff57fa5f13c24a3cafda059f082642952023-09-11T06:59:17ZengSciendoBalkan Journal of Medical Genetics1311-01602017-06-01201273410.1515/bjmg-2017-0009bjmg-2017-0009Molecular and immunohistochemical characteristics of complete hydatidiform molesKubelka-Sabit KB0Prodanova I1Jasar D2Bozinovski G3Filipovski V4Drakulevski S5Plaseska-Karanfilska D6Department of Histopathology and Cytology, Clinical Hospital “Acibadem-Sistina”, Skopje, Republic of MacedoniaDiagnostic Laboratory “Histolab”, Skopje, Republic of MacedoniaDepartment of Histopathology and Cytology, Clinical Hospital “Acibadem-Sistina”, Skopje, Republic of MacedoniaMacedonian Academy of Sciences and Arts, Research Centre for Genetic Engineering and Biotechnology “Georgi D. Efremov”, Skopje, Republic of MacedoniaDepartment of Histopathology and Cytology, Clinical Hospital “Acibadem-Sistina”, Skopje, Republic of MacedoniaDepartment of Histopathology and Cytology, Clinical Hospital “Acibadem-Sistina”, Skopje, Republic of MacedoniaMacedonian Academy of Sciences and Arts, Research Centre for Genetic Engineering and Biotechnology “Georgi D. Efremov”, Skopje, Republic of MacedoniaMolar pregnancy is a gestational trophoblastic disease that belongs to the category of precancerous lesions. On the other end of the spectrum are gestational trophoblastic neoplasms such as invasive mole, choriocarcinoma, placental site trophoblastic tumor and epithelioid trophoblastic tumor, which are considered malignant tumors. Based on defined histopathological criteria, molar pregnancy is divided into partial and complete hydatidiform mole. Especially in the case of early complete mole, the diagnosis can be quite challenging and often necessitates additional molecular or immunohistochemical methods. The aim of this study was to assess the importance of additional molecular and immunohistochemical methods to accurately diagnose complete hydatidiform mole and to stress the importance of correct diagnosis and close follow-up of these patients. A total of 367 consecutive cases of spontaneous abortion were analyzed in a 3-year period. Eight cases with histopathological diagnosis of complete molar pregnancy were selected for further analysis. Apart from standard microscopic analysis, additional molecular and immunohistochemical analyses were performed in all eight cases. Most of the histopathological characteristics of complete molar pregnancy were present in all cases, together with complete absence of positivity for the p57 immunohistochemical marker in the cytotrophoblasts and villous stromal cells. The molecular analysis revealed androgenetic diploidy in seven cases and biparental diploidy in one case with more than three consecutive complete molar pregnancies. Additional immunohistochemical and molecular methods can considerably aid in the correct diagnosis of molar pregnancy.https://doi.org/10.1515/bjmg-2017-0009biparental diploidycomplete molemolecular diagnosticsp57 |
| spellingShingle | Kubelka-Sabit KB Prodanova I Jasar D Bozinovski G Filipovski V Drakulevski S Plaseska-Karanfilska D Molecular and immunohistochemical characteristics of complete hydatidiform moles Balkan Journal of Medical Genetics biparental diploidy complete mole molecular diagnostics p57 |
| title | Molecular and immunohistochemical characteristics of complete hydatidiform moles |
| title_full | Molecular and immunohistochemical characteristics of complete hydatidiform moles |
| title_fullStr | Molecular and immunohistochemical characteristics of complete hydatidiform moles |
| title_full_unstemmed | Molecular and immunohistochemical characteristics of complete hydatidiform moles |
| title_short | Molecular and immunohistochemical characteristics of complete hydatidiform moles |
| title_sort | molecular and immunohistochemical characteristics of complete hydatidiform moles |
| topic | biparental diploidy complete mole molecular diagnostics p57 |
| url | https://doi.org/10.1515/bjmg-2017-0009 |
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