Fabrication of Marsdenia tenacissima with decorated nickel oxide nanoparticles: Investigation of anticancer activity in lung cancer cells and its apoptosis
The possibility of fabricating metal nanoparticles using plant extracts has already been explored in phytomedicines. Nanobiotechnology has a wide range of potential applications, including the cosmetics industry, packaging, coatings, biomedicine, and the enhancement of biological activity. Nickel ox...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Results in Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715623003594 |
| _version_ | 1797398670739505152 |
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| author | Jianhua Deng Daosheng Li Rui Chen Xiangsheng Zeng Yu Liu |
| author_facet | Jianhua Deng Daosheng Li Rui Chen Xiangsheng Zeng Yu Liu |
| author_sort | Jianhua Deng |
| collection | DOAJ |
| description | The possibility of fabricating metal nanoparticles using plant extracts has already been explored in phytomedicines. Nanobiotechnology has a wide range of potential applications, including the cosmetics industry, packaging, coatings, biomedicine, and the enhancement of biological activity. Nickel oxide (NiO) was fabricated in a waste-free, low-cost method and then used to adorn Marsdenia tenacissima extract (MTE), a crucial plant leaf. After forming the nanoparticles, MTE@NiO-NPs were also tested for their optical and biological qualities. The morphological characteristics of NiO-NPs are rhombohedra and slightly agglomerated, and the particle size of the biosynthesized NiO-NPs is observed in the range of ∼ 15 nm. The in vitro cytotoxicity of Marsdenia tenacissima extract (MTE) and nanoparticles on lung cancer cells (A549 and H1299) by MTT assay. The in vitro cytotoxicity assay results revealed that the newly fabricated nanoparticles effectively kill cancer cells with low minimal inhibitory concentration. The various biochemical staining assays assessed the cell death and morphological investigation. Furthermore, the mode of cell death was evaluated by mitochondrial membrane potential (Δψm) and reactive oxygen species (ROS). Therefore, the study concludes that the plant leaves extract of Marsdenia tenacissima is a powerful reducing/capping agent in fabricating MTE@NiO-NPs, demonstrating useful biological features. |
| first_indexed | 2024-03-09T01:28:56Z |
| format | Article |
| id | doaj.art-ff58abc26d6640cd9ebf5ac154153711 |
| institution | Directory Open Access Journal |
| issn | 2211-7156 |
| language | English |
| last_indexed | 2024-03-09T01:28:56Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj.art-ff58abc26d6640cd9ebf5ac1541537112023-12-10T06:15:19ZengElsevierResults in Chemistry2211-71562023-12-016101120Fabrication of Marsdenia tenacissima with decorated nickel oxide nanoparticles: Investigation of anticancer activity in lung cancer cells and its apoptosisJianhua Deng0Daosheng Li1Rui Chen2Xiangsheng Zeng3Yu Liu4Department of Oncology, Jiu Jiang NO. 1 People's Hospital, Jiujiang 332001, ChinaDepartment of Oncology, Jiu Jiang NO. 1 People's Hospital, Jiujiang 332001, ChinaDepartment of Oncology, Jiu Jiang NO. 1 People's Hospital, Jiujiang 332001, ChinaDepartment of Oncology, Jiu Jiang NO. 1 People's Hospital, Jiujiang 332001, ChinaTraditional Chinese Medicine, Jiu Jiang NO.1 People's Hospital, Jiujiang 332001, China; Corresponding author at: No. 48, Taling South Road, Xunyang District, Jiujiang 332001, China.The possibility of fabricating metal nanoparticles using plant extracts has already been explored in phytomedicines. Nanobiotechnology has a wide range of potential applications, including the cosmetics industry, packaging, coatings, biomedicine, and the enhancement of biological activity. Nickel oxide (NiO) was fabricated in a waste-free, low-cost method and then used to adorn Marsdenia tenacissima extract (MTE), a crucial plant leaf. After forming the nanoparticles, MTE@NiO-NPs were also tested for their optical and biological qualities. The morphological characteristics of NiO-NPs are rhombohedra and slightly agglomerated, and the particle size of the biosynthesized NiO-NPs is observed in the range of ∼ 15 nm. The in vitro cytotoxicity of Marsdenia tenacissima extract (MTE) and nanoparticles on lung cancer cells (A549 and H1299) by MTT assay. The in vitro cytotoxicity assay results revealed that the newly fabricated nanoparticles effectively kill cancer cells with low minimal inhibitory concentration. The various biochemical staining assays assessed the cell death and morphological investigation. Furthermore, the mode of cell death was evaluated by mitochondrial membrane potential (Δψm) and reactive oxygen species (ROS). Therefore, the study concludes that the plant leaves extract of Marsdenia tenacissima is a powerful reducing/capping agent in fabricating MTE@NiO-NPs, demonstrating useful biological features.http://www.sciencedirect.com/science/article/pii/S2211715623003594Nickel oxide nanoparticlesMarsdenia tenacissimaIn vitro cytotoxicityLung cancerApoptosis |
| spellingShingle | Jianhua Deng Daosheng Li Rui Chen Xiangsheng Zeng Yu Liu Fabrication of Marsdenia tenacissima with decorated nickel oxide nanoparticles: Investigation of anticancer activity in lung cancer cells and its apoptosis Results in Chemistry Nickel oxide nanoparticles Marsdenia tenacissima In vitro cytotoxicity Lung cancer Apoptosis |
| title | Fabrication of Marsdenia tenacissima with decorated nickel oxide nanoparticles: Investigation of anticancer activity in lung cancer cells and its apoptosis |
| title_full | Fabrication of Marsdenia tenacissima with decorated nickel oxide nanoparticles: Investigation of anticancer activity in lung cancer cells and its apoptosis |
| title_fullStr | Fabrication of Marsdenia tenacissima with decorated nickel oxide nanoparticles: Investigation of anticancer activity in lung cancer cells and its apoptosis |
| title_full_unstemmed | Fabrication of Marsdenia tenacissima with decorated nickel oxide nanoparticles: Investigation of anticancer activity in lung cancer cells and its apoptosis |
| title_short | Fabrication of Marsdenia tenacissima with decorated nickel oxide nanoparticles: Investigation of anticancer activity in lung cancer cells and its apoptosis |
| title_sort | fabrication of marsdenia tenacissima with decorated nickel oxide nanoparticles investigation of anticancer activity in lung cancer cells and its apoptosis |
| topic | Nickel oxide nanoparticles Marsdenia tenacissima In vitro cytotoxicity Lung cancer Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2211715623003594 |
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