Identification of common blood gene signatures for the diagnosis of renal and cardiac acute allograft rejection.
To test, whether 10 genes, diagnostic of renal allograft rejection in blood, are able to diagnose and predict cardiac allograft rejection, we analyzed 250 blood samples from heart transplant recipients with and without acute rejection (AR) and with cytomegalovirus (CMV) infection by QPCR. A QPCR-bas...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3864873?pdf=render |
| _version_ | 1811273647615115264 |
|---|---|
| author | Li Li Kiran Khush Szu-Chuan Hsieh Lihua Ying Helen Luikart Tara Sigdel Silke Roedder Andrew Yang Hannah Valantine Minnie M Sarwal |
| author_facet | Li Li Kiran Khush Szu-Chuan Hsieh Lihua Ying Helen Luikart Tara Sigdel Silke Roedder Andrew Yang Hannah Valantine Minnie M Sarwal |
| author_sort | Li Li |
| collection | DOAJ |
| description | To test, whether 10 genes, diagnostic of renal allograft rejection in blood, are able to diagnose and predict cardiac allograft rejection, we analyzed 250 blood samples from heart transplant recipients with and without acute rejection (AR) and with cytomegalovirus (CMV) infection by QPCR. A QPCR-based logistic regression model was built on 5 of these 10 genes (AR threshold composite score >37% = AR) and tested for AR prediction in an independent set of 109 samples, where it correctly diagnosed AR with 89% accuracy, with no misclassifications for AR ISHLT grade 1b. CMV infection did not confound the AR score. The genes correctly diagnosed AR in a blood sample within 6 months prior to biopsy diagnosis with 80% sensitivity and untreated grade 1b AR episodes had persistently elevated scores until 6 months after biopsy diagnosis. The gene score was also correlated with presence or absence of cardiac allograft vasculopathy (CAV) irrespective of rejection grade. In conclusion, there is a common transcriptional axis of immunological trafficking in peripheral blood in both renal and cardiac organ transplant rejection, across a diverse recipient age range. A common gene signature, initially identified in the setting of renal transplant rejection, can be utilized serially after cardiac transplantation, to diagnose and predict biopsy confirmed acute heart transplant rejection. |
| first_indexed | 2024-04-12T23:04:13Z |
| format | Article |
| id | doaj.art-ff5aee69dbda4bc6beb30743c0f7243e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T23:04:13Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ff5aee69dbda4bc6beb30743c0f7243e2022-12-22T03:12:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8215310.1371/journal.pone.0082153Identification of common blood gene signatures for the diagnosis of renal and cardiac acute allograft rejection.Li LiKiran KhushSzu-Chuan HsiehLihua YingHelen LuikartTara SigdelSilke RoedderAndrew YangHannah ValantineMinnie M SarwalTo test, whether 10 genes, diagnostic of renal allograft rejection in blood, are able to diagnose and predict cardiac allograft rejection, we analyzed 250 blood samples from heart transplant recipients with and without acute rejection (AR) and with cytomegalovirus (CMV) infection by QPCR. A QPCR-based logistic regression model was built on 5 of these 10 genes (AR threshold composite score >37% = AR) and tested for AR prediction in an independent set of 109 samples, where it correctly diagnosed AR with 89% accuracy, with no misclassifications for AR ISHLT grade 1b. CMV infection did not confound the AR score. The genes correctly diagnosed AR in a blood sample within 6 months prior to biopsy diagnosis with 80% sensitivity and untreated grade 1b AR episodes had persistently elevated scores until 6 months after biopsy diagnosis. The gene score was also correlated with presence or absence of cardiac allograft vasculopathy (CAV) irrespective of rejection grade. In conclusion, there is a common transcriptional axis of immunological trafficking in peripheral blood in both renal and cardiac organ transplant rejection, across a diverse recipient age range. A common gene signature, initially identified in the setting of renal transplant rejection, can be utilized serially after cardiac transplantation, to diagnose and predict biopsy confirmed acute heart transplant rejection.http://europepmc.org/articles/PMC3864873?pdf=render |
| spellingShingle | Li Li Kiran Khush Szu-Chuan Hsieh Lihua Ying Helen Luikart Tara Sigdel Silke Roedder Andrew Yang Hannah Valantine Minnie M Sarwal Identification of common blood gene signatures for the diagnosis of renal and cardiac acute allograft rejection. PLoS ONE |
| title | Identification of common blood gene signatures for the diagnosis of renal and cardiac acute allograft rejection. |
| title_full | Identification of common blood gene signatures for the diagnosis of renal and cardiac acute allograft rejection. |
| title_fullStr | Identification of common blood gene signatures for the diagnosis of renal and cardiac acute allograft rejection. |
| title_full_unstemmed | Identification of common blood gene signatures for the diagnosis of renal and cardiac acute allograft rejection. |
| title_short | Identification of common blood gene signatures for the diagnosis of renal and cardiac acute allograft rejection. |
| title_sort | identification of common blood gene signatures for the diagnosis of renal and cardiac acute allograft rejection |
| url | http://europepmc.org/articles/PMC3864873?pdf=render |
| work_keys_str_mv | AT lili identificationofcommonbloodgenesignaturesforthediagnosisofrenalandcardiacacuteallograftrejection AT kirankhush identificationofcommonbloodgenesignaturesforthediagnosisofrenalandcardiacacuteallograftrejection AT szuchuanhsieh identificationofcommonbloodgenesignaturesforthediagnosisofrenalandcardiacacuteallograftrejection AT lihuaying identificationofcommonbloodgenesignaturesforthediagnosisofrenalandcardiacacuteallograftrejection AT helenluikart identificationofcommonbloodgenesignaturesforthediagnosisofrenalandcardiacacuteallograftrejection AT tarasigdel identificationofcommonbloodgenesignaturesforthediagnosisofrenalandcardiacacuteallograftrejection AT silkeroedder identificationofcommonbloodgenesignaturesforthediagnosisofrenalandcardiacacuteallograftrejection AT andrewyang identificationofcommonbloodgenesignaturesforthediagnosisofrenalandcardiacacuteallograftrejection AT hannahvalantine identificationofcommonbloodgenesignaturesforthediagnosisofrenalandcardiacacuteallograftrejection AT minniemsarwal identificationofcommonbloodgenesignaturesforthediagnosisofrenalandcardiacacuteallograftrejection |