Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues
Human bone is a specialized tissue with unique material properties, providing mechanical support and resistance to the skeleton and simultaneously assuring capability of adaptation and remodelling. Knowing the properties of such a structure down to the micro-scale is of utmost importance, not only f...
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MDPI AG
2021-11-01
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| Online Access: | https://www.mdpi.com/1996-1944/14/22/6869 |
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| author | Martina Alunni Cardinali Assunta Morresi Daniele Fioretto Leonardo Vivarelli Dante Dallari Marco Govoni |
| author_facet | Martina Alunni Cardinali Assunta Morresi Daniele Fioretto Leonardo Vivarelli Dante Dallari Marco Govoni |
| author_sort | Martina Alunni Cardinali |
| collection | DOAJ |
| description | Human bone is a specialized tissue with unique material properties, providing mechanical support and resistance to the skeleton and simultaneously assuring capability of adaptation and remodelling. Knowing the properties of such a structure down to the micro-scale is of utmost importance, not only for the design of effective biomimetic materials but also to be able to detect pathological alterations in material properties, such as micro-fractures or abnormal tissue remodelling. The Brillouin and Raman micro-spectroscopic (BRmS) approach has the potential to become a first-choice technique, as it is capable of simultaneously investigating samples’ mechanical and structural properties in a non-destructive and label-free way. Here, we perform a mapping of cortical and trabecular bone sections of a femoral epiphysis, demonstrating the capability of the technique for discovering the morpho-mechanics of cells, the extracellular matrix, and marrow constituents. Moreover, the interpretation of Brillouin and Raman spectra merged with an approach of data mining is used to compare the mechanical alterations in specimens excised from distinct anatomical areas and subjected to different sample processing. The results disclose in both cases specific alterations in the morphology and/or in the tissue chemical make-up, which strongly affects bone mechanical properties, providing a method potentially extendable to other important biomedical issues. |
| first_indexed | 2024-03-10T05:20:19Z |
| format | Article |
| id | doaj.art-ff5b81f508b84f2187b55e63adff7857 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T05:20:19Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-ff5b81f508b84f2187b55e63adff78572023-11-23T00:10:03ZengMDPI AGMaterials1996-19442021-11-011422686910.3390/ma14226869Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone TissuesMartina Alunni Cardinali0Assunta Morresi1Daniele Fioretto2Leonardo Vivarelli3Dante Dallari4Marco Govoni5Department of Physics and Geology, University of Perugia, Via A. Pascoli, I-06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, I-06123 Perugia, ItalyDepartment of Physics and Geology, University of Perugia, Via A. Pascoli, I-06123 Perugia, ItalyReconstructive Orthopaedic Surgery and Innovative Techniques—Musculoskeletal Tissue Bank, IRCCS Istituto Ortopedico Rizzoli, Via G.C. Pupilli 1, 40136 Bologna, ItalyReconstructive Orthopaedic Surgery and Innovative Techniques—Musculoskeletal Tissue Bank, IRCCS Istituto Ortopedico Rizzoli, Via G.C. Pupilli 1, 40136 Bologna, ItalyReconstructive Orthopaedic Surgery and Innovative Techniques—Musculoskeletal Tissue Bank, IRCCS Istituto Ortopedico Rizzoli, Via G.C. Pupilli 1, 40136 Bologna, ItalyHuman bone is a specialized tissue with unique material properties, providing mechanical support and resistance to the skeleton and simultaneously assuring capability of adaptation and remodelling. Knowing the properties of such a structure down to the micro-scale is of utmost importance, not only for the design of effective biomimetic materials but also to be able to detect pathological alterations in material properties, such as micro-fractures or abnormal tissue remodelling. The Brillouin and Raman micro-spectroscopic (BRmS) approach has the potential to become a first-choice technique, as it is capable of simultaneously investigating samples’ mechanical and structural properties in a non-destructive and label-free way. Here, we perform a mapping of cortical and trabecular bone sections of a femoral epiphysis, demonstrating the capability of the technique for discovering the morpho-mechanics of cells, the extracellular matrix, and marrow constituents. Moreover, the interpretation of Brillouin and Raman spectra merged with an approach of data mining is used to compare the mechanical alterations in specimens excised from distinct anatomical areas and subjected to different sample processing. The results disclose in both cases specific alterations in the morphology and/or in the tissue chemical make-up, which strongly affects bone mechanical properties, providing a method potentially extendable to other important biomedical issues.https://www.mdpi.com/1996-1944/14/22/6869Raman spectroscopyBrillouin spectroscopybone material characterizationbone micro-scale architecture |
| spellingShingle | Martina Alunni Cardinali Assunta Morresi Daniele Fioretto Leonardo Vivarelli Dante Dallari Marco Govoni Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues Materials Raman spectroscopy Brillouin spectroscopy bone material characterization bone micro-scale architecture |
| title | Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues |
| title_full | Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues |
| title_fullStr | Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues |
| title_full_unstemmed | Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues |
| title_short | Brillouin and Raman Micro-Spectroscopy: A Tool for Micro-Mechanical and Structural Characterization of Cortical and Trabecular Bone Tissues |
| title_sort | brillouin and raman micro spectroscopy a tool for micro mechanical and structural characterization of cortical and trabecular bone tissues |
| topic | Raman spectroscopy Brillouin spectroscopy bone material characterization bone micro-scale architecture |
| url | https://www.mdpi.com/1996-1944/14/22/6869 |
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