Implication of Different Tumor Biomarkers in Drug Resistance and Invasiveness in Primary and Metastatic Colorectal Cancer Cell Lines
Protein expression profiles are directly related to the different properties of cells and are conditioned by the cellular niche. As an example, they are the cause of the characteristic cell plasticity, epithelium–mesenchymal transition (EMT), and drug resistance of cancer cells. This article charact...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/5/1083 |
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| author | Marta Sánchez-Díez Nicolás Alegría-Aravena Marta López-Montes Josefa Quiroz-Troncoso Raquel González-Martos Adrián Menéndez-Rey José Luis Sánchez-Sánchez Juan Manuel Pastor Carmen Ramírez-Castillejo |
| author_facet | Marta Sánchez-Díez Nicolás Alegría-Aravena Marta López-Montes Josefa Quiroz-Troncoso Raquel González-Martos Adrián Menéndez-Rey José Luis Sánchez-Sánchez Juan Manuel Pastor Carmen Ramírez-Castillejo |
| author_sort | Marta Sánchez-Díez |
| collection | DOAJ |
| description | Protein expression profiles are directly related to the different properties of cells and are conditioned by the cellular niche. As an example, they are the cause of the characteristic cell plasticity, epithelium–mesenchymal transition (EMT), and drug resistance of cancer cells. This article characterizes ten biomarkers related to these features in three human colorectal cancer cell lines: SW-480, SW-620, and DLD-1, evaluated by flow cytometry; and in turn, resistance to oxaliplatin is studied through dose–response trials. The main biomarkers present in the three studied lines correspond to EpCAM, CD-133, and AC-133, with the latter two in low proportions in the DLD-1 line. The biomarker CD166 is present in greater amounts in SW-620 and DLD-1 compared to SW-480. Finally, DLD-1 shows high values of Trop2, which may explain the aggressiveness and resistance of these cells to oxaliplatin treatments, as EpCAM is also highly expressed. Exposure to oxaliplatin slows cell growth but also helps generate resistance to the treatment. In conclusion, the response of the cell lines is variable, due to their genetic variability, which will condition protein expression and cell growth. Further analyses in this area will provide important information for better understanding of patients’ cellular response and how to prevent resistance. |
| first_indexed | 2024-03-10T03:18:03Z |
| format | Article |
| id | doaj.art-ff64eff4aef246beadec109d4dc51142 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T03:18:03Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-ff64eff4aef246beadec109d4dc511422023-11-23T10:10:47ZengMDPI AGBiomedicines2227-90592022-05-01105108310.3390/biomedicines10051083Implication of Different Tumor Biomarkers in Drug Resistance and Invasiveness in Primary and Metastatic Colorectal Cancer Cell LinesMarta Sánchez-Díez0Nicolás Alegría-Aravena1Marta López-Montes2Josefa Quiroz-Troncoso3Raquel González-Martos4Adrián Menéndez-Rey5José Luis Sánchez-Sánchez6Juan Manuel Pastor7Carmen Ramírez-Castillejo8CTB (CTB-UPM) Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón, SpainCTB (CTB-UPM) Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón, SpainCTB (CTB-UPM) Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón, SpainCTB (CTB-UPM) Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón, SpainCTB (CTB-UPM) Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón, SpainCTB (CTB-UPM) Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón, SpainUnidad de Oncología, Hospital de Almansa, 02640 Albacete, SpainGrupo de Sistemas Complejos, Universidad Politécnica de Madrid, 28040 Madrid, SpainCTB (CTB-UPM) Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón, SpainProtein expression profiles are directly related to the different properties of cells and are conditioned by the cellular niche. As an example, they are the cause of the characteristic cell plasticity, epithelium–mesenchymal transition (EMT), and drug resistance of cancer cells. This article characterizes ten biomarkers related to these features in three human colorectal cancer cell lines: SW-480, SW-620, and DLD-1, evaluated by flow cytometry; and in turn, resistance to oxaliplatin is studied through dose–response trials. The main biomarkers present in the three studied lines correspond to EpCAM, CD-133, and AC-133, with the latter two in low proportions in the DLD-1 line. The biomarker CD166 is present in greater amounts in SW-620 and DLD-1 compared to SW-480. Finally, DLD-1 shows high values of Trop2, which may explain the aggressiveness and resistance of these cells to oxaliplatin treatments, as EpCAM is also highly expressed. Exposure to oxaliplatin slows cell growth but also helps generate resistance to the treatment. In conclusion, the response of the cell lines is variable, due to their genetic variability, which will condition protein expression and cell growth. Further analyses in this area will provide important information for better understanding of patients’ cellular response and how to prevent resistance.https://www.mdpi.com/2227-9059/10/5/1083epithelium–mesenchymal transitioncell plasticitycancer stem cellstumor biomarkersresistancechemotherapy |
| spellingShingle | Marta Sánchez-Díez Nicolás Alegría-Aravena Marta López-Montes Josefa Quiroz-Troncoso Raquel González-Martos Adrián Menéndez-Rey José Luis Sánchez-Sánchez Juan Manuel Pastor Carmen Ramírez-Castillejo Implication of Different Tumor Biomarkers in Drug Resistance and Invasiveness in Primary and Metastatic Colorectal Cancer Cell Lines Biomedicines epithelium–mesenchymal transition cell plasticity cancer stem cells tumor biomarkers resistance chemotherapy |
| title | Implication of Different Tumor Biomarkers in Drug Resistance and Invasiveness in Primary and Metastatic Colorectal Cancer Cell Lines |
| title_full | Implication of Different Tumor Biomarkers in Drug Resistance and Invasiveness in Primary and Metastatic Colorectal Cancer Cell Lines |
| title_fullStr | Implication of Different Tumor Biomarkers in Drug Resistance and Invasiveness in Primary and Metastatic Colorectal Cancer Cell Lines |
| title_full_unstemmed | Implication of Different Tumor Biomarkers in Drug Resistance and Invasiveness in Primary and Metastatic Colorectal Cancer Cell Lines |
| title_short | Implication of Different Tumor Biomarkers in Drug Resistance and Invasiveness in Primary and Metastatic Colorectal Cancer Cell Lines |
| title_sort | implication of different tumor biomarkers in drug resistance and invasiveness in primary and metastatic colorectal cancer cell lines |
| topic | epithelium–mesenchymal transition cell plasticity cancer stem cells tumor biomarkers resistance chemotherapy |
| url | https://www.mdpi.com/2227-9059/10/5/1083 |
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