HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of <i>Sorghum bicolor</i> during Germination
The purpose of this study was to assess the suitability of the Romanian Albanus hybrid of <i>Sorghum bicolor</i> as a potential functional food ingredient. Ultrasound-assisted extraction in different solvents, together with spectrophotometric and chromatographic methods, was used to moni...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/3/417 |
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| author | Ioana Otilia Ghinea Maria Daniela Ionica Mihaila Giorgiana-Valentina Blaga (Costea) Sorin Marius Avramescu Mihaela Cudalbeanu Simona-Florina Isticioaia Rodica Mihaela Dinica Bianca Furdui |
| author_facet | Ioana Otilia Ghinea Maria Daniela Ionica Mihaila Giorgiana-Valentina Blaga (Costea) Sorin Marius Avramescu Mihaela Cudalbeanu Simona-Florina Isticioaia Rodica Mihaela Dinica Bianca Furdui |
| author_sort | Ioana Otilia Ghinea |
| collection | DOAJ |
| description | The purpose of this study was to assess the suitability of the Romanian Albanus hybrid of <i>Sorghum bicolor</i> as a potential functional food ingredient. Ultrasound-assisted extraction in different solvents, together with spectrophotometric and chromatographic methods, was used to monitor the variation in total phenolic and flavonoid content and the antioxidant activity of raw sorghum grains before and during short germination periods (24, 36 and 48 h). The High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) investigation of the extracts revealed that the ungerminated sorghum grains extracted with methanol had the highest diversity of phenolic compounds, while the total phenolic content (TPC) was the highest after 36 h of germination in both extract types: 1853 mg GAE/100 g for the methanolic extract and 1726 mg GAE/100 g for the ethanolic extract. The findings of this study showed that the TPC of sorghum extracts is strongly correlated with their antioxidant activity and, overall, that the studied extracts presented a good radical scavenger activity, which supports the benefits of alimentary uses of <i>Sorghum bicolor</i> grains. |
| first_indexed | 2024-03-09T00:34:09Z |
| format | Article |
| id | doaj.art-ff67dffe8223463a95bada21024e4fd2 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T00:34:09Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-ff67dffe8223463a95bada21024e4fd22023-12-11T18:20:09ZengMDPI AGAgronomy2073-43952021-02-0111341710.3390/agronomy11030417HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of <i>Sorghum bicolor</i> during GerminationIoana Otilia Ghinea0Maria Daniela Ionica Mihaila1Giorgiana-Valentina Blaga (Costea)2Sorin Marius Avramescu3Mihaela Cudalbeanu4Simona-Florina Isticioaia5Rodica Mihaela Dinica6Bianca Furdui7Faculty of Sciences and Environment, Department of Chemistry Physical and Environment, “Dunarea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati, RomaniaFaculty of Sciences and Environment, Department of Chemistry Physical and Environment, “Dunarea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati, RomaniaFaculty of Food Science and Engineering, Department of Food Science, Food Engineering, Biotechnology and Aquaculture, “Dunarea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati, RomaniaFaculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, University of Bucharest, 90–92 Soseaua Panduri, 050663 Bucharest, RomaniaFaculty of Sciences and Environment, Department of Chemistry Physical and Environment, “Dunarea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati, RomaniaAgricultural Research and Development Station, Secuieni, 617415 Neamț, RomaniaFaculty of Sciences and Environment, Department of Chemistry Physical and Environment, “Dunarea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati, RomaniaFaculty of Sciences and Environment, Department of Chemistry Physical and Environment, “Dunarea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati, RomaniaThe purpose of this study was to assess the suitability of the Romanian Albanus hybrid of <i>Sorghum bicolor</i> as a potential functional food ingredient. Ultrasound-assisted extraction in different solvents, together with spectrophotometric and chromatographic methods, was used to monitor the variation in total phenolic and flavonoid content and the antioxidant activity of raw sorghum grains before and during short germination periods (24, 36 and 48 h). The High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) investigation of the extracts revealed that the ungerminated sorghum grains extracted with methanol had the highest diversity of phenolic compounds, while the total phenolic content (TPC) was the highest after 36 h of germination in both extract types: 1853 mg GAE/100 g for the methanolic extract and 1726 mg GAE/100 g for the ethanolic extract. The findings of this study showed that the TPC of sorghum extracts is strongly correlated with their antioxidant activity and, overall, that the studied extracts presented a good radical scavenger activity, which supports the benefits of alimentary uses of <i>Sorghum bicolor</i> grains.https://www.mdpi.com/2073-4395/11/3/417sorghum graingerminationphenolic contentflavonoidsantioxidant activity |
| spellingShingle | Ioana Otilia Ghinea Maria Daniela Ionica Mihaila Giorgiana-Valentina Blaga (Costea) Sorin Marius Avramescu Mihaela Cudalbeanu Simona-Florina Isticioaia Rodica Mihaela Dinica Bianca Furdui HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of <i>Sorghum bicolor</i> during Germination Agronomy sorghum grain germination phenolic content flavonoids antioxidant activity |
| title | HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of <i>Sorghum bicolor</i> during Germination |
| title_full | HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of <i>Sorghum bicolor</i> during Germination |
| title_fullStr | HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of <i>Sorghum bicolor</i> during Germination |
| title_full_unstemmed | HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of <i>Sorghum bicolor</i> during Germination |
| title_short | HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of <i>Sorghum bicolor</i> during Germination |
| title_sort | hplc dad polyphenolic profiling and antioxidant activities of i sorghum bicolor i during germination |
| topic | sorghum grain germination phenolic content flavonoids antioxidant activity |
| url | https://www.mdpi.com/2073-4395/11/3/417 |
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