Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petunia
Rosa rugosa is an important natural perfume plant in China. Rose essential oil is known as ‘liquid gold’ and has high economic and health values. Monoterpenes are the main fragrant components of R. rugosa flower and essential oil. In this study, a member of the hydrolase gene family RrNUDX1 was clon...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2021-04-01
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| Online Access: | https://peerj.com/articles/11098.pdf |
| _version_ | 1797418223609577472 |
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| author | Lixia Sheng Shu Zang Jianwen Wang Tiantian Wei Yong Xu Liguo Feng |
| author_facet | Lixia Sheng Shu Zang Jianwen Wang Tiantian Wei Yong Xu Liguo Feng |
| author_sort | Lixia Sheng |
| collection | DOAJ |
| description | Rosa rugosa is an important natural perfume plant in China. Rose essential oil is known as ‘liquid gold’ and has high economic and health values. Monoterpenes are the main fragrant components of R. rugosa flower and essential oil. In this study, a member of the hydrolase gene family RrNUDX1 was cloned from Chinese traditional R. rugosa ‘Tang Hong’. Combined analysis of RrNUDX1 gene expression and the aroma components in different development stages and different parts of flower organ, we found that the main aroma component content was consistent with the gene expression pattern. The RrNUDX1 overexpressed Petunia hybrida was acquired via Agrobacterium-mediated genetic transformation systems. The blades of the transgenic petunias became wider and its growth vigor became strong with stronger fragrance. Gas chromatography with mass spectrometry analysis showed that the contents of the main aroma components of the transgenic petunias including methyl benzoate significantly increased. These findings indicate that the RrNUDX1 gene plays a role in enhancing the fragrance of petunia flowers, and they could lay an important foundation for the homeotic transformation of RrNUDX1 in R. rugosa for cultivating new R. rugosa varieties of high-yield and -quality essential oil. |
| first_indexed | 2024-03-09T06:30:36Z |
| format | Article |
| id | doaj.art-ff6877be2959422da3504f8e07bc2030 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:30:36Z |
| publishDate | 2021-04-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-ff6877be2959422da3504f8e07bc20302023-12-03T11:10:00ZengPeerJ Inc.PeerJ2167-83592021-04-019e1109810.7717/peerj.11098Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petuniaLixia Sheng0Shu Zang1Jianwen Wang2Tiantian Wei3Yong Xu4Liguo Feng5College of Horticulture and Plant Protection, Yanghzou University, Yangzhou, Jiangsu, ChinaCollege of Horticulture and Plant Protection, Yanghzou University, Yangzhou, Jiangsu, ChinaCollege of Horticulture and Plant Protection, Yanghzou University, Yangzhou, Jiangsu, ChinaCollege of Horticulture and Plant Protection, Yanghzou University, Yangzhou, Jiangsu, ChinaCollege of Horticulture and Plant Protection, Yanghzou University, Yangzhou, Jiangsu, ChinaCollege of Horticulture and Plant Protection, Yanghzou University, Yangzhou, Jiangsu, ChinaRosa rugosa is an important natural perfume plant in China. Rose essential oil is known as ‘liquid gold’ and has high economic and health values. Monoterpenes are the main fragrant components of R. rugosa flower and essential oil. In this study, a member of the hydrolase gene family RrNUDX1 was cloned from Chinese traditional R. rugosa ‘Tang Hong’. Combined analysis of RrNUDX1 gene expression and the aroma components in different development stages and different parts of flower organ, we found that the main aroma component content was consistent with the gene expression pattern. The RrNUDX1 overexpressed Petunia hybrida was acquired via Agrobacterium-mediated genetic transformation systems. The blades of the transgenic petunias became wider and its growth vigor became strong with stronger fragrance. Gas chromatography with mass spectrometry analysis showed that the contents of the main aroma components of the transgenic petunias including methyl benzoate significantly increased. These findings indicate that the RrNUDX1 gene plays a role in enhancing the fragrance of petunia flowers, and they could lay an important foundation for the homeotic transformation of RrNUDX1 in R. rugosa for cultivating new R. rugosa varieties of high-yield and -quality essential oil.https://peerj.com/articles/11098.pdfNUDXRosa rugosaVolatile monoterpenes |
| spellingShingle | Lixia Sheng Shu Zang Jianwen Wang Tiantian Wei Yong Xu Liguo Feng Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petunia PeerJ NUDX Rosa rugosa Volatile monoterpenes |
| title | Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petunia |
| title_full | Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petunia |
| title_fullStr | Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petunia |
| title_full_unstemmed | Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petunia |
| title_short | Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petunia |
| title_sort | overexpression of a rosa rugosa thunb nudx gene enhances biosynthesis of scent volatiles in petunia |
| topic | NUDX Rosa rugosa Volatile monoterpenes |
| url | https://peerj.com/articles/11098.pdf |
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