Application of RNA-Seq transcriptome analysis: CD151 is an Invasion/Migration target in all stages of epithelial ovarian cancer
<p>Abstract</p> <p>Background</p> <p>RNA-Seq allows a theoretically unbiased analysis of both genome-wide transcription levels and mutation status of a tumor. Using this technique we sought to identify novel candidate therapeutic targets expressed in epithelial ovarian...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-01-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://www.ovarianresearch.com/content/5/1/4 |
| _version_ | 1828082682835238912 |
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| author | Mosig Rebecca A Lin Li Senturk Emir Shah Hardik Huang Fei Schlosshauer Peter Cohen Samantha Fruscio Robert Marchini Sergio D'Incalci Maurizio Sachidanandam Ravi Dottino Peter Martignetti John A |
| author_facet | Mosig Rebecca A Lin Li Senturk Emir Shah Hardik Huang Fei Schlosshauer Peter Cohen Samantha Fruscio Robert Marchini Sergio D'Incalci Maurizio Sachidanandam Ravi Dottino Peter Martignetti John A |
| author_sort | Mosig Rebecca A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>RNA-Seq allows a theoretically unbiased analysis of both genome-wide transcription levels and mutation status of a tumor. Using this technique we sought to identify novel candidate therapeutic targets expressed in epithelial ovarian cancer (EOC).</p> <p>Methods</p> <p>Specifically, we sought candidate invasion/migration targets based on expression levels across all tumors, novelty of expression in EOC, and known function. RNA-Seq analysis revealed the high expression of CD151, a transmembrane protein, across all stages of EOC. Expression was confirmed at both the mRNA and protein levels using RT-PCR and immunohistochemical staining, respectively.</p> <p>Results</p> <p>In both EOC tumors and normal ovarian surface epithelial cells we demonstrated CD151 to be localized to the membrane and cell-cell junctions in patient-derived and established EOC cell lines. We next evaluated its role in EOC dissemination using two ovarian cancer-derived cell lines with differential levels of CD151 expression. Targeted antibody-mediated and siRNA inhibition or loss of CD151 in SKOV3 and OVCAR5 cell lines effectively inhibited their migration and invasion.</p> <p>Conclusion</p> <p>Taken together, these findings provide the first proof-of-principle demonstration for a next generation sequencing approach to identifying candidate therapeutic targets and reveal CD151 to play a role in EOC dissemination.</p> |
| first_indexed | 2024-04-11T03:55:51Z |
| format | Article |
| id | doaj.art-ff693e0a1aab497ca4913c51d0812b83 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T03:55:51Z |
| publishDate | 2012-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-ff693e0a1aab497ca4913c51d0812b832023-01-02T00:37:16ZengBMCJournal of Ovarian Research1757-22152012-01-0151410.1186/1757-2215-5-4Application of RNA-Seq transcriptome analysis: CD151 is an Invasion/Migration target in all stages of epithelial ovarian cancerMosig Rebecca ALin LiSenturk EmirShah HardikHuang FeiSchlosshauer PeterCohen SamanthaFruscio RobertMarchini SergioD'Incalci MaurizioSachidanandam RaviDottino PeterMartignetti John A<p>Abstract</p> <p>Background</p> <p>RNA-Seq allows a theoretically unbiased analysis of both genome-wide transcription levels and mutation status of a tumor. Using this technique we sought to identify novel candidate therapeutic targets expressed in epithelial ovarian cancer (EOC).</p> <p>Methods</p> <p>Specifically, we sought candidate invasion/migration targets based on expression levels across all tumors, novelty of expression in EOC, and known function. RNA-Seq analysis revealed the high expression of CD151, a transmembrane protein, across all stages of EOC. Expression was confirmed at both the mRNA and protein levels using RT-PCR and immunohistochemical staining, respectively.</p> <p>Results</p> <p>In both EOC tumors and normal ovarian surface epithelial cells we demonstrated CD151 to be localized to the membrane and cell-cell junctions in patient-derived and established EOC cell lines. We next evaluated its role in EOC dissemination using two ovarian cancer-derived cell lines with differential levels of CD151 expression. Targeted antibody-mediated and siRNA inhibition or loss of CD151 in SKOV3 and OVCAR5 cell lines effectively inhibited their migration and invasion.</p> <p>Conclusion</p> <p>Taken together, these findings provide the first proof-of-principle demonstration for a next generation sequencing approach to identifying candidate therapeutic targets and reveal CD151 to play a role in EOC dissemination.</p>http://www.ovarianresearch.com/content/5/1/4CD151Epithelial Ovarian CancerInvasionMigrationMetastasisRNA-Seq |
| spellingShingle | Mosig Rebecca A Lin Li Senturk Emir Shah Hardik Huang Fei Schlosshauer Peter Cohen Samantha Fruscio Robert Marchini Sergio D'Incalci Maurizio Sachidanandam Ravi Dottino Peter Martignetti John A Application of RNA-Seq transcriptome analysis: CD151 is an Invasion/Migration target in all stages of epithelial ovarian cancer Journal of Ovarian Research CD151 Epithelial Ovarian Cancer Invasion Migration Metastasis RNA-Seq |
| title | Application of RNA-Seq transcriptome analysis: CD151 is an Invasion/Migration target in all stages of epithelial ovarian cancer |
| title_full | Application of RNA-Seq transcriptome analysis: CD151 is an Invasion/Migration target in all stages of epithelial ovarian cancer |
| title_fullStr | Application of RNA-Seq transcriptome analysis: CD151 is an Invasion/Migration target in all stages of epithelial ovarian cancer |
| title_full_unstemmed | Application of RNA-Seq transcriptome analysis: CD151 is an Invasion/Migration target in all stages of epithelial ovarian cancer |
| title_short | Application of RNA-Seq transcriptome analysis: CD151 is an Invasion/Migration target in all stages of epithelial ovarian cancer |
| title_sort | application of rna seq transcriptome analysis cd151 is an invasion migration target in all stages of epithelial ovarian cancer |
| topic | CD151 Epithelial Ovarian Cancer Invasion Migration Metastasis RNA-Seq |
| url | http://www.ovarianresearch.com/content/5/1/4 |
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