Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus
Loading frequency is known to influence the expression of the focal adhesions of the adherent cells. A small cyclical tensile force was transmitted to mouse pre-osteoblast MC3T3-E1 cells through PDMS substrates of varying stiffness. Changes in cell behavior with respect to proliferation and characte...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340915003479 |
| _version_ | 1819106462355423232 |
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| author | J.J. Cora-Cruz N. Diffoot-Carlo P.A. Sundaram |
| author_facet | J.J. Cora-Cruz N. Diffoot-Carlo P.A. Sundaram |
| author_sort | J.J. Cora-Cruz |
| collection | DOAJ |
| description | Loading frequency is known to influence the expression of the focal adhesions of the adherent cells. A small cyclical tensile force was transmitted to mouse pre-osteoblast MC3T3-E1 cells through PDMS substrates of varying stiffness. Changes in cell behavior with respect to proliferation and characteristics of focal adhesions were quantified through immunofluorescence labeling of vinculin. Amount of inactive vinculin was higher on substrates subjected to cyclic stimulation when compared with the results of the static substrates, whereas the number and area of focal adhesion points underwent a reduction. Inactive vinculin appears as a cloud in the cytoplasm in the vicinity of the nucleus. Keywords: MC3T3-E1 Cells, Mechanical Stimulus, Focal Adhesions, Vinculin |
| first_indexed | 2024-12-22T02:38:31Z |
| format | Article |
| id | doaj.art-ff6af7e55bd84938847ffa2ea9c46fdb |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-22T02:38:31Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-ff6af7e55bd84938847ffa2ea9c46fdb2022-12-21T18:41:42ZengElsevierData in Brief2352-34092016-03-01694100Vinculin expression in MC3T3-E1 cells in response to mechanical stimulusJ.J. Cora-Cruz0N. Diffoot-Carlo1P.A. Sundaram2Department of Mechanical Engineering, University of Puerto Rico: Mayagüez Campus, Mayagüez, PR 00680, USADepartment of Biology, University of Puerto Rico: Mayagüez Campus, Mayagüez, PR 00680, USADepartment of Mechanical Engineering, University of Puerto Rico: Mayagüez Campus, Mayagüez, PR 00680, USA; Corresponding author.Loading frequency is known to influence the expression of the focal adhesions of the adherent cells. A small cyclical tensile force was transmitted to mouse pre-osteoblast MC3T3-E1 cells through PDMS substrates of varying stiffness. Changes in cell behavior with respect to proliferation and characteristics of focal adhesions were quantified through immunofluorescence labeling of vinculin. Amount of inactive vinculin was higher on substrates subjected to cyclic stimulation when compared with the results of the static substrates, whereas the number and area of focal adhesion points underwent a reduction. Inactive vinculin appears as a cloud in the cytoplasm in the vicinity of the nucleus. Keywords: MC3T3-E1 Cells, Mechanical Stimulus, Focal Adhesions, Vinculinhttp://www.sciencedirect.com/science/article/pii/S2352340915003479 |
| spellingShingle | J.J. Cora-Cruz N. Diffoot-Carlo P.A. Sundaram Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus Data in Brief |
| title | Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus |
| title_full | Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus |
| title_fullStr | Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus |
| title_full_unstemmed | Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus |
| title_short | Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus |
| title_sort | vinculin expression in mc3t3 e1 cells in response to mechanical stimulus |
| url | http://www.sciencedirect.com/science/article/pii/S2352340915003479 |
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