Coptis chinensis, and extracts of guava and mulberry leaves present good inhibiting potential on obesity and associated metabolic disorders in high-fat diet obesity mice model
This study aimed to investigate the anti-obesity effects of Coptis chinensis (CC), BALASAN (combinational guava leaf extract and mulberry leaf extract), and CC/BALASAN (CC/BAL) on high-fat diet-induced obese C57BL/6 mice and to explore possible mediating mechanisms in 3T3-L1 pre-adipocytes. Oil red-...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Journal of Traditional and Complementary Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411023000147 |
| _version_ | 1797842276723982336 |
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| author | Pao-Chuan Hsieh Wu-Yuan Chen Tzu-Ming Wang Yung-Lin Chu |
| author_facet | Pao-Chuan Hsieh Wu-Yuan Chen Tzu-Ming Wang Yung-Lin Chu |
| author_sort | Pao-Chuan Hsieh |
| collection | DOAJ |
| description | This study aimed to investigate the anti-obesity effects of Coptis chinensis (CC), BALASAN (combinational guava leaf extract and mulberry leaf extract), and CC/BALASAN (CC/BAL) on high-fat diet-induced obese C57BL/6 mice and to explore possible mediating mechanisms in 3T3-L1 pre-adipocytes. Oil red-O stain was used to test the effects of CC, BALASAN, and CC/BAL on the differentiation of 3T3-L1 pre-adipocytes. Additionally, real-time PCR was used to detect the expression of genes involved in adipocyte differentiation and inflammation-related genes in adipose tissue of mice that were fed a high-fat diet. CC, BALASAN, and CC/BAL inhibited the differentiation of 3T3-L1 pre-adipocytes and exhibited excellent inhibitory ability against the expression of PPARγ and RXRα genes associated with adipocyte differentiation. Replenishing mice with a high-fat diet with CC, BALASAN, and CC/BAL reduced body weight gaining and blood glucose and plasma cholesterol levels. CC also effectively reduced liver weight, whereas BALASAN and CC/BAL had no inhibitory effect. In addition, CC effectively inhibited the expression of C/EBP-α in adipose tissue. Interestingly, BALASAN not only inhibited the expression of C/EBP-α, but also that of PPARγ, RXRα, and TNFα. Such data indicated that CC, BALASAN, and CC/BAL may have potentially beneficial effects against obesity and associated metabolic disorders by down-regulating the PPARγ/RXRα pathway. |
| first_indexed | 2024-04-09T16:45:17Z |
| format | Article |
| id | doaj.art-ff6cbde6e1f546488beba0f85ee7fe18 |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-04-09T16:45:17Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-ff6cbde6e1f546488beba0f85ee7fe182023-04-23T06:06:20ZengElsevierJournal of Traditional and Complementary Medicine2225-41102023-05-01133270276Coptis chinensis, and extracts of guava and mulberry leaves present good inhibiting potential on obesity and associated metabolic disorders in high-fat diet obesity mice modelPao-Chuan Hsieh0Wu-Yuan Chen1Tzu-Ming Wang2Yung-Lin Chu3Department of Food Science, College of Agriculture, National Pingtung University of Science and Technology, No.1, Shuefu Rd., Neipu Township, Pingtung County, 91201, TaiwanPD BIOTECH CO., LTD, Pingtung County, TaiwanDepartment of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung County, 80756, TaiwanDepartment of Food Science, College of Agriculture, National Pingtung University of Science and Technology, No.1, Shuefu Rd., Neipu Township, Pingtung County, 91201, Taiwan; Corresponding author.This study aimed to investigate the anti-obesity effects of Coptis chinensis (CC), BALASAN (combinational guava leaf extract and mulberry leaf extract), and CC/BALASAN (CC/BAL) on high-fat diet-induced obese C57BL/6 mice and to explore possible mediating mechanisms in 3T3-L1 pre-adipocytes. Oil red-O stain was used to test the effects of CC, BALASAN, and CC/BAL on the differentiation of 3T3-L1 pre-adipocytes. Additionally, real-time PCR was used to detect the expression of genes involved in adipocyte differentiation and inflammation-related genes in adipose tissue of mice that were fed a high-fat diet. CC, BALASAN, and CC/BAL inhibited the differentiation of 3T3-L1 pre-adipocytes and exhibited excellent inhibitory ability against the expression of PPARγ and RXRα genes associated with adipocyte differentiation. Replenishing mice with a high-fat diet with CC, BALASAN, and CC/BAL reduced body weight gaining and blood glucose and plasma cholesterol levels. CC also effectively reduced liver weight, whereas BALASAN and CC/BAL had no inhibitory effect. In addition, CC effectively inhibited the expression of C/EBP-α in adipose tissue. Interestingly, BALASAN not only inhibited the expression of C/EBP-α, but also that of PPARγ, RXRα, and TNFα. Such data indicated that CC, BALASAN, and CC/BAL may have potentially beneficial effects against obesity and associated metabolic disorders by down-regulating the PPARγ/RXRα pathway.http://www.sciencedirect.com/science/article/pii/S2225411023000147Coptis chinensisBALASAN3T3-L1 pre-adipocytesAnti-obesityAdipocyte differentiation genesAnti-inflammation |
| spellingShingle | Pao-Chuan Hsieh Wu-Yuan Chen Tzu-Ming Wang Yung-Lin Chu Coptis chinensis, and extracts of guava and mulberry leaves present good inhibiting potential on obesity and associated metabolic disorders in high-fat diet obesity mice model Journal of Traditional and Complementary Medicine Coptis chinensis BALASAN 3T3-L1 pre-adipocytes Anti-obesity Adipocyte differentiation genes Anti-inflammation |
| title | Coptis chinensis, and extracts of guava and mulberry leaves present good inhibiting potential on obesity and associated metabolic disorders in high-fat diet obesity mice model |
| title_full | Coptis chinensis, and extracts of guava and mulberry leaves present good inhibiting potential on obesity and associated metabolic disorders in high-fat diet obesity mice model |
| title_fullStr | Coptis chinensis, and extracts of guava and mulberry leaves present good inhibiting potential on obesity and associated metabolic disorders in high-fat diet obesity mice model |
| title_full_unstemmed | Coptis chinensis, and extracts of guava and mulberry leaves present good inhibiting potential on obesity and associated metabolic disorders in high-fat diet obesity mice model |
| title_short | Coptis chinensis, and extracts of guava and mulberry leaves present good inhibiting potential on obesity and associated metabolic disorders in high-fat diet obesity mice model |
| title_sort | coptis chinensis and extracts of guava and mulberry leaves present good inhibiting potential on obesity and associated metabolic disorders in high fat diet obesity mice model |
| topic | Coptis chinensis BALASAN 3T3-L1 pre-adipocytes Anti-obesity Adipocyte differentiation genes Anti-inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S2225411023000147 |
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