Unraveling the superiority of (−)‐gallocatechin gallate to (−)‐epigallocatechin‐3‐gallate in protection of diabetic nephropathy of db/db mice
Abstract GCG ((−)‐gallocatechin gallate) and EGCG ((−)‐epigallocatechin‐3‐gallate) are main biologically active polyphenolic compounds in tea leaves, and EGCG has been studied for its potential effects on alleviating diabetic nephropathy (DN). Previous studies showed that GCG exhibited better hypogl...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-03-01
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| Series: | Food Frontiers |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fft2.356 |
| _version_ | 1797257854753701888 |
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| author | Xin Xiao Ling Feng Huijun Cheng Huifang Ge Yijun Wang Xiaochun Wan Daxiang Li Zhongwen Xie |
| author_facet | Xin Xiao Ling Feng Huijun Cheng Huifang Ge Yijun Wang Xiaochun Wan Daxiang Li Zhongwen Xie |
| author_sort | Xin Xiao |
| collection | DOAJ |
| description | Abstract GCG ((−)‐gallocatechin gallate) and EGCG ((−)‐epigallocatechin‐3‐gallate) are main biologically active polyphenolic compounds in tea leaves, and EGCG has been studied for its potential effects on alleviating diabetic nephropathy (DN). Previous studies showed that GCG exhibited better hypoglycemic and antioxidant efficacy than EGCG. However, the efficacy and molecular mechanisms of GCG in the protection of DN are unknown. In this study, db/db mice were administered with EGCG or GCG orally for 20 weeks to comparatively investigate their effects on DN. These results revealed that GCG was significantly more effective than EGCG in decreasing water intake and urine excretion, reducing fasting blood glucose levels and systolic blood pressure, improving mesenteric artery contractility, enhancing the right renal hemodynamics, and improving key renal function markers. Through transcriptome analysis, S100A8/S100A9, two regulators of interstitial fibrosis, were identified and found to be more sensitive to GCG intervention, which was further confirmed by mRNA and protein expression data. In addition, the molecular docking analysis results demonstrated that GCG displayed a superior binding affinity toward S100A8/S100A9 in comparison to EGCG. Overall, our study found that GCG holds significant potential for the treatment of DN and outperforms EGCG in terms of efficacy. |
| first_indexed | 2024-04-24T22:44:16Z |
| format | Article |
| id | doaj.art-ff6ccb96d659472abbfa9a65e7627cce |
| institution | Directory Open Access Journal |
| issn | 2643-8429 |
| language | English |
| last_indexed | 2024-04-24T22:44:16Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Frontiers |
| spelling | doaj.art-ff6ccb96d659472abbfa9a65e7627cce2024-03-18T16:46:15ZengWileyFood Frontiers2643-84292024-03-015277178810.1002/fft2.356Unraveling the superiority of (−)‐gallocatechin gallate to (−)‐epigallocatechin‐3‐gallate in protection of diabetic nephropathy of db/db miceXin Xiao0Ling Feng1Huijun Cheng2Huifang Ge3Yijun Wang4Xiaochun Wan5Daxiang Li6Zhongwen Xie7State Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University Hefei ChinaState Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University Hefei ChinaSchool of Life Sciences Anhui Agricultural University Hefei ChinaState Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University Hefei ChinaState Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University Hefei ChinaState Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University Hefei ChinaState Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University Hefei ChinaState Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University Hefei ChinaAbstract GCG ((−)‐gallocatechin gallate) and EGCG ((−)‐epigallocatechin‐3‐gallate) are main biologically active polyphenolic compounds in tea leaves, and EGCG has been studied for its potential effects on alleviating diabetic nephropathy (DN). Previous studies showed that GCG exhibited better hypoglycemic and antioxidant efficacy than EGCG. However, the efficacy and molecular mechanisms of GCG in the protection of DN are unknown. In this study, db/db mice were administered with EGCG or GCG orally for 20 weeks to comparatively investigate their effects on DN. These results revealed that GCG was significantly more effective than EGCG in decreasing water intake and urine excretion, reducing fasting blood glucose levels and systolic blood pressure, improving mesenteric artery contractility, enhancing the right renal hemodynamics, and improving key renal function markers. Through transcriptome analysis, S100A8/S100A9, two regulators of interstitial fibrosis, were identified and found to be more sensitive to GCG intervention, which was further confirmed by mRNA and protein expression data. In addition, the molecular docking analysis results demonstrated that GCG displayed a superior binding affinity toward S100A8/S100A9 in comparison to EGCG. Overall, our study found that GCG holds significant potential for the treatment of DN and outperforms EGCG in terms of efficacy.https://doi.org/10.1002/fft2.356comparisondb/dbdiabetic nephropathyEGCGGCGtranscriptome analysis |
| spellingShingle | Xin Xiao Ling Feng Huijun Cheng Huifang Ge Yijun Wang Xiaochun Wan Daxiang Li Zhongwen Xie Unraveling the superiority of (−)‐gallocatechin gallate to (−)‐epigallocatechin‐3‐gallate in protection of diabetic nephropathy of db/db mice Food Frontiers comparison db/db diabetic nephropathy EGCG GCG transcriptome analysis |
| title | Unraveling the superiority of (−)‐gallocatechin gallate to (−)‐epigallocatechin‐3‐gallate in protection of diabetic nephropathy of db/db mice |
| title_full | Unraveling the superiority of (−)‐gallocatechin gallate to (−)‐epigallocatechin‐3‐gallate in protection of diabetic nephropathy of db/db mice |
| title_fullStr | Unraveling the superiority of (−)‐gallocatechin gallate to (−)‐epigallocatechin‐3‐gallate in protection of diabetic nephropathy of db/db mice |
| title_full_unstemmed | Unraveling the superiority of (−)‐gallocatechin gallate to (−)‐epigallocatechin‐3‐gallate in protection of diabetic nephropathy of db/db mice |
| title_short | Unraveling the superiority of (−)‐gallocatechin gallate to (−)‐epigallocatechin‐3‐gallate in protection of diabetic nephropathy of db/db mice |
| title_sort | unraveling the superiority of gallocatechin gallate to epigallocatechin 3 gallate in protection of diabetic nephropathy of db db mice |
| topic | comparison db/db diabetic nephropathy EGCG GCG transcriptome analysis |
| url | https://doi.org/10.1002/fft2.356 |
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