Naoxintong capsules exhibited a protective effect against cerebral ischemia in the brain hub region of MCAO rats via intervention of amino acid metabolism
Background: Amino acids (AAs) metabolism profiles in brain play a pivotal role in cerebral ischemia (CI), which is a complex, spatial and temporal event. NaoXinTong Capsules (NXT) are widely prescribed in Chinese medicine for the treatment of cerebrovascular and cardiovascular diseases. Methods: In...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-04-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535224000613 |
| _version_ | 1797248385392050176 |
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| author | Huanhuan Wang Jingtong Liu Mengli Chang Guanghuan Tian Xixian Kong Mengting Liu Na Guo Liying Tang Jing Xu Hongwei Wu Hongjun Yang |
| author_facet | Huanhuan Wang Jingtong Liu Mengli Chang Guanghuan Tian Xixian Kong Mengting Liu Na Guo Liying Tang Jing Xu Hongwei Wu Hongjun Yang |
| author_sort | Huanhuan Wang |
| collection | DOAJ |
| description | Background: Amino acids (AAs) metabolism profiles in brain play a pivotal role in cerebral ischemia (CI), which is a complex, spatial and temporal event. NaoXinTong Capsules (NXT) are widely prescribed in Chinese medicine for the treatment of cerebrovascular and cardiovascular diseases. Methods: In this study, a novel analytical method for the quantification of twenty-three AAs in cortex and hippocampus was developed by UPLC-QQQ-MS. Based on middle cerebral artery occlusion (MCAO) rat model, the cerebral infarction and the neurological behavior scores are firstly dynamically evaluated at 3, 12, and 24 h after CI. Then the dynamic levels of the AAs in cortex and hippocampus were analyzed respectively after CI. Results: After CI, MCAO rats showed neurological deficits especially at 24 h. Based on PCA scores plot at different time after CI, the time of the AAs metabolism showing an obvious disturbance in the cortex (12 and 24 h after ischemia) is earlier than that in the hippocampus (24 h after ischemia). Based on the fold-changes of MCAO vs. sham group and receiver operating curve (ROC) analysis, a total of nine AAs biomarkers, with continuous and consistent changes at different time points after CI, were finally obtained including eight AAs of Ala, GABA, Pro, Val, Ile, His, Cit and Leu in the cortex, and three AAs of Ile, Asp, and Cit in the hippocampus. After NXT treatment, the efficacy was confirmed by reducing cerebral infarction and improving the neurological behavior scores. Furthermore, GABA, Pro, His, Leu, Asp, Ile and Cit were found to exhibited an obvious tendency for returning to baseline values in the corresponding regions at 24 h after CI (P < 0.05). Conclusion: This study provides new strategies to explore the mechanism of CI and help discover the potential mechanism of NXT. |
| first_indexed | 2024-03-08T02:02:00Z |
| format | Article |
| id | doaj.art-ff6f08ca341a4fc78e93c275427066ac |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-24T20:13:45Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-ff6f08ca341a4fc78e93c275427066ac2024-03-23T06:23:35ZengElsevierArabian Journal of Chemistry1878-53522024-04-01174105659Naoxintong capsules exhibited a protective effect against cerebral ischemia in the brain hub region of MCAO rats via intervention of amino acid metabolismHuanhuan Wang0Jingtong Liu1Mengli Chang2Guanghuan Tian3Xixian Kong4Mengting Liu5Na Guo6Liying Tang7Jing Xu8Hongwei Wu9Hongjun Yang10Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; Hospital of Integrated Chinese and Western Medicine, Tianjin university of TCM, Tianjin 300100, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaZhejiang Chinese Medical University, Zhejiang Province 310053, ChinaExperimental Research Centre, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; Corresponding authors.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; Corresponding authors.Experimental Research Centre, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaBackground: Amino acids (AAs) metabolism profiles in brain play a pivotal role in cerebral ischemia (CI), which is a complex, spatial and temporal event. NaoXinTong Capsules (NXT) are widely prescribed in Chinese medicine for the treatment of cerebrovascular and cardiovascular diseases. Methods: In this study, a novel analytical method for the quantification of twenty-three AAs in cortex and hippocampus was developed by UPLC-QQQ-MS. Based on middle cerebral artery occlusion (MCAO) rat model, the cerebral infarction and the neurological behavior scores are firstly dynamically evaluated at 3, 12, and 24 h after CI. Then the dynamic levels of the AAs in cortex and hippocampus were analyzed respectively after CI. Results: After CI, MCAO rats showed neurological deficits especially at 24 h. Based on PCA scores plot at different time after CI, the time of the AAs metabolism showing an obvious disturbance in the cortex (12 and 24 h after ischemia) is earlier than that in the hippocampus (24 h after ischemia). Based on the fold-changes of MCAO vs. sham group and receiver operating curve (ROC) analysis, a total of nine AAs biomarkers, with continuous and consistent changes at different time points after CI, were finally obtained including eight AAs of Ala, GABA, Pro, Val, Ile, His, Cit and Leu in the cortex, and three AAs of Ile, Asp, and Cit in the hippocampus. After NXT treatment, the efficacy was confirmed by reducing cerebral infarction and improving the neurological behavior scores. Furthermore, GABA, Pro, His, Leu, Asp, Ile and Cit were found to exhibited an obvious tendency for returning to baseline values in the corresponding regions at 24 h after CI (P < 0.05). Conclusion: This study provides new strategies to explore the mechanism of CI and help discover the potential mechanism of NXT.http://www.sciencedirect.com/science/article/pii/S1878535224000613Cerebral ischemiaAmino acidsLC-QQQ-MSNaoxintong capsulesMetabonomic |
| spellingShingle | Huanhuan Wang Jingtong Liu Mengli Chang Guanghuan Tian Xixian Kong Mengting Liu Na Guo Liying Tang Jing Xu Hongwei Wu Hongjun Yang Naoxintong capsules exhibited a protective effect against cerebral ischemia in the brain hub region of MCAO rats via intervention of amino acid metabolism Arabian Journal of Chemistry Cerebral ischemia Amino acids LC-QQQ-MS Naoxintong capsules Metabonomic |
| title | Naoxintong capsules exhibited a protective effect against cerebral ischemia in the brain hub region of MCAO rats via intervention of amino acid metabolism |
| title_full | Naoxintong capsules exhibited a protective effect against cerebral ischemia in the brain hub region of MCAO rats via intervention of amino acid metabolism |
| title_fullStr | Naoxintong capsules exhibited a protective effect against cerebral ischemia in the brain hub region of MCAO rats via intervention of amino acid metabolism |
| title_full_unstemmed | Naoxintong capsules exhibited a protective effect against cerebral ischemia in the brain hub region of MCAO rats via intervention of amino acid metabolism |
| title_short | Naoxintong capsules exhibited a protective effect against cerebral ischemia in the brain hub region of MCAO rats via intervention of amino acid metabolism |
| title_sort | naoxintong capsules exhibited a protective effect against cerebral ischemia in the brain hub region of mcao rats via intervention of amino acid metabolism |
| topic | Cerebral ischemia Amino acids LC-QQQ-MS Naoxintong capsules Metabonomic |
| url | http://www.sciencedirect.com/science/article/pii/S1878535224000613 |
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