Genomic characters of Anaplasma bovis and genetic diversity in China
ABSTRACTThe emergence of Anaplasma bovis or A. bovis-like infection in humans from China and the United States of America has raised concern about the public health importance of this pathogen. Although A. bovis has been detected in a wide range of ticks and mammals in the world, no genome of the pa...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2024.2323153 |
| _version_ | 1827328645341904896 |
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| author | Xiao-Yu Han Li-Feng Du Zhe-Tao Lin Cheng Li Tao Xiong Wen-Jie Zhu Run-Ze Ye Ning Wang Yi-Fei Wang Wan-Ying Gao Lin Zhao Xiao-Ming Cui Wu-Chun Cao |
| author_facet | Xiao-Yu Han Li-Feng Du Zhe-Tao Lin Cheng Li Tao Xiong Wen-Jie Zhu Run-Ze Ye Ning Wang Yi-Fei Wang Wan-Ying Gao Lin Zhao Xiao-Ming Cui Wu-Chun Cao |
| author_sort | Xiao-Yu Han |
| collection | DOAJ |
| description | ABSTRACTThe emergence of Anaplasma bovis or A. bovis-like infection in humans from China and the United States of America has raised concern about the public health importance of this pathogen. Although A. bovis has been detected in a wide range of ticks and mammals in the world, no genome of the pathogen is available up to now, which has prohibited us from better understanding the genetic basis for its pathogenicity. Here we describe an A. bovis genome from metagenomic sequencing of an infected goat in China. Anaplasma bovis had the smallest genome of the genus Anaplasma, and relatively lower GC content. Phylogenetic analysis of single-copy orthologue sequence showed that A. bovis was closely related to A. platys and A. phagocytophilum, but relatively far from intraerythrocytic Anaplasma species. Anaplasma bovis had 116 unique orthogroups and lacked 51 orthogroups in comparison to other Anaplasma species. The virulence factors of A. bovis were significantly less than those of A. phagocytophilum, suggesting less pathogenicity of A. bovis. When tested by specific PCR assays, A. bovis was detected in 23 of 29 goats, with an infection rate up to 79.3% (95% CI: 64.6% ∼94.1%). The phylogenetic analyses based on partial 16S rRNA, gltA and groEL genes indicated that A. bovis had high genetic diversity. The findings of this study lay a foundation for further understanding of the biological characteristics and genetic diversity of A. bovis, and will facilitate the formulation of prevention and control strategies. |
| first_indexed | 2024-03-07T15:20:32Z |
| format | Article |
| id | doaj.art-ff6fec15a9424e7ea917b5e8f32411a3 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-07T15:20:32Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-ff6fec15a9424e7ea917b5e8f32411a32024-03-05T17:41:27ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512024-12-0113110.1080/22221751.2024.2323153Genomic characters of Anaplasma bovis and genetic diversity in ChinaXiao-Yu Han0Li-Feng Du1Zhe-Tao Lin2Cheng Li3Tao Xiong4Wen-Jie Zhu5Run-Ze Ye6Ning Wang7Yi-Fei Wang8Wan-Ying Gao9Lin Zhao10Xiao-Ming Cui11Wu-Chun Cao12State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of ChinaInstitute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaABSTRACTThe emergence of Anaplasma bovis or A. bovis-like infection in humans from China and the United States of America has raised concern about the public health importance of this pathogen. Although A. bovis has been detected in a wide range of ticks and mammals in the world, no genome of the pathogen is available up to now, which has prohibited us from better understanding the genetic basis for its pathogenicity. Here we describe an A. bovis genome from metagenomic sequencing of an infected goat in China. Anaplasma bovis had the smallest genome of the genus Anaplasma, and relatively lower GC content. Phylogenetic analysis of single-copy orthologue sequence showed that A. bovis was closely related to A. platys and A. phagocytophilum, but relatively far from intraerythrocytic Anaplasma species. Anaplasma bovis had 116 unique orthogroups and lacked 51 orthogroups in comparison to other Anaplasma species. The virulence factors of A. bovis were significantly less than those of A. phagocytophilum, suggesting less pathogenicity of A. bovis. When tested by specific PCR assays, A. bovis was detected in 23 of 29 goats, with an infection rate up to 79.3% (95% CI: 64.6% ∼94.1%). The phylogenetic analyses based on partial 16S rRNA, gltA and groEL genes indicated that A. bovis had high genetic diversity. The findings of this study lay a foundation for further understanding of the biological characteristics and genetic diversity of A. bovis, and will facilitate the formulation of prevention and control strategies.https://www.tandfonline.com/doi/10.1080/22221751.2024.2323153Anaplasma bovistick-borne diseasewhole-genome sequencinggenetic diversitygoatChina |
| spellingShingle | Xiao-Yu Han Li-Feng Du Zhe-Tao Lin Cheng Li Tao Xiong Wen-Jie Zhu Run-Ze Ye Ning Wang Yi-Fei Wang Wan-Ying Gao Lin Zhao Xiao-Ming Cui Wu-Chun Cao Genomic characters of Anaplasma bovis and genetic diversity in China Emerging Microbes and Infections Anaplasma bovis tick-borne disease whole-genome sequencing genetic diversity goat China |
| title | Genomic characters of Anaplasma bovis and genetic diversity in China |
| title_full | Genomic characters of Anaplasma bovis and genetic diversity in China |
| title_fullStr | Genomic characters of Anaplasma bovis and genetic diversity in China |
| title_full_unstemmed | Genomic characters of Anaplasma bovis and genetic diversity in China |
| title_short | Genomic characters of Anaplasma bovis and genetic diversity in China |
| title_sort | genomic characters of anaplasma bovis and genetic diversity in china |
| topic | Anaplasma bovis tick-borne disease whole-genome sequencing genetic diversity goat China |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2024.2323153 |
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