Bioconversion of Glycerol into Lactic Acid by a New Bacterial Strain from the Brazilian Cerrado Soil
A lactic-acid-producing strain was isolated from the Brazilian Cerrado soil (Brazilian savanna). Glycerol, a byproduct of the biodiesel industry, can be converted into various chemical intermediates of industrial value by biotechnological routes. <i>Klebsiella pneumoniae</i> can metaboli...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/10/477 |
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| author | Raissa G. M. R. Barroso Jamille R. C. Lima Léia C. L. Fávaro Fabricio Machado Sílvia B. Gonçalves |
| author_facet | Raissa G. M. R. Barroso Jamille R. C. Lima Léia C. L. Fávaro Fabricio Machado Sílvia B. Gonçalves |
| author_sort | Raissa G. M. R. Barroso |
| collection | DOAJ |
| description | A lactic-acid-producing strain was isolated from the Brazilian Cerrado soil (Brazilian savanna). Glycerol, a byproduct of the biodiesel industry, can be converted into various chemical intermediates of industrial value by biotechnological routes. <i>Klebsiella pneumoniae</i> can metabolize glycerol in environments with or without oxygen and bioconvert it into several chemicals with high value-added, such as lactic acid, 3-hydroxypropionic acid and 1,3 propanediol. The wild-type bacterial strain (2GPP) isolated from a soil sample from the Brazilian Cerrado was determined to be a <i>K. pneumoniae</i> complex that was capable of successfully metabolizing glycerol. Fermentations were performed with different temperatures, pH, and inoculum concentrations to evaluate the best lactic acid production. At first, 1,3-propanediol and L-(+)-lactic acid were produced in mini reactors. A lactic acid production of 3.8 g·L<sup>−1</sup> and a decrease in 1,3-propanediol output were observed. Thus, by adjusting process variables such as pH and temperature during fermentation, it was possible to maximize the production of lactic acid and decrease the formation of 1,3-propanediol by utilizing experimental design strategies. |
| first_indexed | 2024-03-09T12:20:25Z |
| format | Article |
| id | doaj.art-ff711a7c789e4aee993e46609ec3a55e |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T12:20:25Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-ff711a7c789e4aee993e46609ec3a55e2023-11-30T22:41:48ZengMDPI AGFermentation2311-56372022-09-0181047710.3390/fermentation8100477Bioconversion of Glycerol into Lactic Acid by a New Bacterial Strain from the Brazilian Cerrado SoilRaissa G. M. R. Barroso0Jamille R. C. Lima1Léia C. L. Fávaro2Fabricio Machado3Sílvia B. Gonçalves4Instituto de Química, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília 70910-900, BrazilEmbrapa Agroenergia, Parque Estação Biológica, PqEb s/n, W3 Norte, Brasília 70770-901, BrazilEmbrapa Agroenergia, Parque Estação Biológica, PqEb s/n, W3 Norte, Brasília 70770-901, BrazilInstituto de Química, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília 70910-900, BrazilEmbrapa Agroenergia, Parque Estação Biológica, PqEb s/n, W3 Norte, Brasília 70770-901, BrazilA lactic-acid-producing strain was isolated from the Brazilian Cerrado soil (Brazilian savanna). Glycerol, a byproduct of the biodiesel industry, can be converted into various chemical intermediates of industrial value by biotechnological routes. <i>Klebsiella pneumoniae</i> can metabolize glycerol in environments with or without oxygen and bioconvert it into several chemicals with high value-added, such as lactic acid, 3-hydroxypropionic acid and 1,3 propanediol. The wild-type bacterial strain (2GPP) isolated from a soil sample from the Brazilian Cerrado was determined to be a <i>K. pneumoniae</i> complex that was capable of successfully metabolizing glycerol. Fermentations were performed with different temperatures, pH, and inoculum concentrations to evaluate the best lactic acid production. At first, 1,3-propanediol and L-(+)-lactic acid were produced in mini reactors. A lactic acid production of 3.8 g·L<sup>−1</sup> and a decrease in 1,3-propanediol output were observed. Thus, by adjusting process variables such as pH and temperature during fermentation, it was possible to maximize the production of lactic acid and decrease the formation of 1,3-propanediol by utilizing experimental design strategies.https://www.mdpi.com/2311-5637/8/10/477lactic acidglycerol<i>Klebsiella pneumoniae</i>fermentationbrazilian cerrado soil |
| spellingShingle | Raissa G. M. R. Barroso Jamille R. C. Lima Léia C. L. Fávaro Fabricio Machado Sílvia B. Gonçalves Bioconversion of Glycerol into Lactic Acid by a New Bacterial Strain from the Brazilian Cerrado Soil Fermentation lactic acid glycerol <i>Klebsiella pneumoniae</i> fermentation brazilian cerrado soil |
| title | Bioconversion of Glycerol into Lactic Acid by a New Bacterial Strain from the Brazilian Cerrado Soil |
| title_full | Bioconversion of Glycerol into Lactic Acid by a New Bacterial Strain from the Brazilian Cerrado Soil |
| title_fullStr | Bioconversion of Glycerol into Lactic Acid by a New Bacterial Strain from the Brazilian Cerrado Soil |
| title_full_unstemmed | Bioconversion of Glycerol into Lactic Acid by a New Bacterial Strain from the Brazilian Cerrado Soil |
| title_short | Bioconversion of Glycerol into Lactic Acid by a New Bacterial Strain from the Brazilian Cerrado Soil |
| title_sort | bioconversion of glycerol into lactic acid by a new bacterial strain from the brazilian cerrado soil |
| topic | lactic acid glycerol <i>Klebsiella pneumoniae</i> fermentation brazilian cerrado soil |
| url | https://www.mdpi.com/2311-5637/8/10/477 |
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