Protocol for transplanting pancreatic islets into the parametrial fat pad of female mice
Summary: Although the male epididymal fat pad is an effective site for islet transplantation, females lack this tissue. Here, we present a protocol to assess the parametrial fat pad (PFP) adjacent to the uterine horn in females as an alternative site for islet transplantation. We describe steps for...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723007839 |
| _version_ | 1827390430187094016 |
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| author | Mebrahtu G. Tedla Nathaniel Wright Esma S. Yolcu Yadong Wang Haval Shirwan |
| author_facet | Mebrahtu G. Tedla Nathaniel Wright Esma S. Yolcu Yadong Wang Haval Shirwan |
| author_sort | Mebrahtu G. Tedla |
| collection | DOAJ |
| description | Summary: Although the male epididymal fat pad is an effective site for islet transplantation, females lack this tissue. Here, we present a protocol to assess the parametrial fat pad (PFP) adjacent to the uterine horn in females as an alternative site for islet transplantation. We describe steps for islet isolation from the pancreas, counting, transplantation into PFP, and monitoring for engraftment. Transplantation into PFP is minimally invasive, time efficient, and supports long-term engraftment of syngeneic islets and rejection of allogeneic islets.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-08T16:51:36Z |
| format | Article |
| id | doaj.art-ff71f8f3228141d7bbe73edf05f49dc2 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-08T16:51:36Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-ff71f8f3228141d7bbe73edf05f49dc22024-01-05T04:25:13ZengElsevierSTAR Protocols2666-16672024-03-0151102816Protocol for transplanting pancreatic islets into the parametrial fat pad of female miceMebrahtu G. Tedla0Nathaniel Wright1Esma S. Yolcu2Yadong Wang3Haval Shirwan4Department of Pediatrics, Ellis Fischel Cancer Center, School of Medicine, University of Missouri, Columbia, MO 65211, USA; Department of Molecular Microbiology and Immunology, Ellis Fischel Cancer Center, School of Medicine, University of Missouri, Columbia, MO 65211, USA; NextGen Precision Health Institute, Ellis Fischel Cancer Center, School of Medicine, University of Missouri, Columbia, MO 65211, USAMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USADepartment of Pediatrics, Ellis Fischel Cancer Center, School of Medicine, University of Missouri, Columbia, MO 65211, USA; Department of Molecular Microbiology and Immunology, Ellis Fischel Cancer Center, School of Medicine, University of Missouri, Columbia, MO 65211, USA; NextGen Precision Health Institute, Ellis Fischel Cancer Center, School of Medicine, University of Missouri, Columbia, MO 65211, USAMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USADepartment of Pediatrics, Ellis Fischel Cancer Center, School of Medicine, University of Missouri, Columbia, MO 65211, USA; Corresponding authorSummary: Although the male epididymal fat pad is an effective site for islet transplantation, females lack this tissue. Here, we present a protocol to assess the parametrial fat pad (PFP) adjacent to the uterine horn in females as an alternative site for islet transplantation. We describe steps for islet isolation from the pancreas, counting, transplantation into PFP, and monitoring for engraftment. Transplantation into PFP is minimally invasive, time efficient, and supports long-term engraftment of syngeneic islets and rejection of allogeneic islets.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723007839Cell isolationHealth SciencesImmunologyMetabolismModel Organisms |
| spellingShingle | Mebrahtu G. Tedla Nathaniel Wright Esma S. Yolcu Yadong Wang Haval Shirwan Protocol for transplanting pancreatic islets into the parametrial fat pad of female mice STAR Protocols Cell isolation Health Sciences Immunology Metabolism Model Organisms |
| title | Protocol for transplanting pancreatic islets into the parametrial fat pad of female mice |
| title_full | Protocol for transplanting pancreatic islets into the parametrial fat pad of female mice |
| title_fullStr | Protocol for transplanting pancreatic islets into the parametrial fat pad of female mice |
| title_full_unstemmed | Protocol for transplanting pancreatic islets into the parametrial fat pad of female mice |
| title_short | Protocol for transplanting pancreatic islets into the parametrial fat pad of female mice |
| title_sort | protocol for transplanting pancreatic islets into the parametrial fat pad of female mice |
| topic | Cell isolation Health Sciences Immunology Metabolism Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723007839 |
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