A new toxic protein from death cap Amanita phalloides: isolation and study of cytotoxic activity
Cytotoxic protein from fruit body of the death cap Amanita phalloides mushroom, designated as toxophallin, has been isolated and its principal physico-chemical and biological properties have been characterized. This protein possesses molecular mass 55 kDa and isoelectric point at pH 5.7 and is expre...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Львівський національний університет імені Івана Франка
2008-09-01
|
| Series: | Біологічні студії |
| Subjects: | |
| Online Access: | http://publications.lnu.edu.ua/journals/index.php/biology/article/view/496 |
| _version_ | 1818365854157373440 |
|---|---|
| author | T. Stasyk M. Lootsik U. Hellman Ch. Wernstedt S. Souchelnytskyi R. Stoika |
| author_facet | T. Stasyk M. Lootsik U. Hellman Ch. Wernstedt S. Souchelnytskyi R. Stoika |
| author_sort | T. Stasyk |
| collection | DOAJ |
| description | Cytotoxic protein from fruit body of the death cap Amanita phalloides mushroom, designated as toxophallin, has been isolated and its principal physico-chemical and biological properties have been characterized. This protein possesses molecular mass 55 kDa and isoelectric point at pH 5.7 and is expressed predominantly in the stem of mushroom fruit body. As assessed by cross-linking experiments, toxophallin forms a trimeric complex in buffered solution. It induces apoptosis in several mammalian cells lines (murine L1210 and CCL-64, and human A549) with IC50 at 0.25–0.45 ug/ml. It also induces DNA fragmentation and morphological changes in the nuclei of target cells (chromatin condensation and fragmentation of nuclei) that are characteristic for apoptosis. Caspase III Inhibitor (Boc-D-fmk) does not inhibit toxophallin-induced apoptotic DNA fragmentation, suggesting that this toxin involves caspase-independent pathway of apoptosis. Besides, it was shown that toxophallin interaction with target cells is not mediated by specific cell surface receptor. Thus, toxophallin is a new toxic protein whose properties distinguish it from other toxic compounds (cyclopeptides and phallolysin) earlier found in the death cap. |
| first_indexed | 2024-12-13T22:26:52Z |
| format | Article |
| id | doaj.art-ff7516c8e55641dc8a1378d900f50805 |
| institution | Directory Open Access Journal |
| issn | 1996-4536 2311-0783 |
| language | English |
| last_indexed | 2024-12-13T22:26:52Z |
| publishDate | 2008-09-01 |
| publisher | Львівський національний університет імені Івана Франка |
| record_format | Article |
| series | Біологічні студії |
| spelling | doaj.art-ff7516c8e55641dc8a1378d900f508052022-12-21T23:29:12ZengЛьвівський національний університет імені Івана ФранкаБіологічні студії1996-45362311-07832008-09-0121213210.30970/sbi.0201.023A new toxic protein from death cap Amanita phalloides: isolation and study of cytotoxic activityT. Stasyk0https://orcid.org/0000-0003-0568-6841M. Lootsik1https://orcid.org/0000-0001-7018-5408U. Hellman2Ch. Wernstedt3S. Souchelnytskyi4https://orcid.org/0000-0001-8243-9276R. Stoika5https://orcid.org/0000-0001-5719-2187Institute of Cell Biology, NAS of Ukraine; Biocenter, Innsbruck Medical University, AustriaInstitute of Cell Biology, NAS of UkraineLudwig Institute for Cancer Research, Uppsala, SwedenLudwig Institute for Cancer Research, Uppsala, SwedenKarolinska Biomics Center, Karolinska Institutet, Stockholm, SwedenInstitute of Cell Biology, NAS of UkraineCytotoxic protein from fruit body of the death cap Amanita phalloides mushroom, designated as toxophallin, has been isolated and its principal physico-chemical and biological properties have been characterized. This protein possesses molecular mass 55 kDa and isoelectric point at pH 5.7 and is expressed predominantly in the stem of mushroom fruit body. As assessed by cross-linking experiments, toxophallin forms a trimeric complex in buffered solution. It induces apoptosis in several mammalian cells lines (murine L1210 and CCL-64, and human A549) with IC50 at 0.25–0.45 ug/ml. It also induces DNA fragmentation and morphological changes in the nuclei of target cells (chromatin condensation and fragmentation of nuclei) that are characteristic for apoptosis. Caspase III Inhibitor (Boc-D-fmk) does not inhibit toxophallin-induced apoptotic DNA fragmentation, suggesting that this toxin involves caspase-independent pathway of apoptosis. Besides, it was shown that toxophallin interaction with target cells is not mediated by specific cell surface receptor. Thus, toxophallin is a new toxic protein whose properties distinguish it from other toxic compounds (cyclopeptides and phallolysin) earlier found in the death cap.http://publications.lnu.edu.ua/journals/index.php/biology/article/view/496toxic proteindeath cap amanita phalloidesapoptosis |
| spellingShingle | T. Stasyk M. Lootsik U. Hellman Ch. Wernstedt S. Souchelnytskyi R. Stoika A new toxic protein from death cap Amanita phalloides: isolation and study of cytotoxic activity Біологічні студії toxic protein death cap amanita phalloides apoptosis |
| title | A new toxic protein from death cap Amanita phalloides: isolation and study of cytotoxic activity |
| title_full | A new toxic protein from death cap Amanita phalloides: isolation and study of cytotoxic activity |
| title_fullStr | A new toxic protein from death cap Amanita phalloides: isolation and study of cytotoxic activity |
| title_full_unstemmed | A new toxic protein from death cap Amanita phalloides: isolation and study of cytotoxic activity |
| title_short | A new toxic protein from death cap Amanita phalloides: isolation and study of cytotoxic activity |
| title_sort | new toxic protein from death cap amanita phalloides isolation and study of cytotoxic activity |
| topic | toxic protein death cap amanita phalloides apoptosis |
| url | http://publications.lnu.edu.ua/journals/index.php/biology/article/view/496 |
| work_keys_str_mv | AT tstasyk anewtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity AT mlootsik anewtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity AT uhellman anewtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity AT chwernstedt anewtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity AT ssouchelnytskyi anewtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity AT rstoika anewtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity AT tstasyk newtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity AT mlootsik newtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity AT uhellman newtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity AT chwernstedt newtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity AT ssouchelnytskyi newtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity AT rstoika newtoxicproteinfromdeathcapamanitaphalloidesisolationandstudyofcytotoxicactivity |