Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering
Recently, many research groups have investigated three-dimensional (3D) bioprinting techniques for tissue engineering and regenerative medicine. The bio-ink used in 3D bioprinting is typically a combination of synthetic and natural materials. In this study, we prepared bio-ink containing porcine ski...
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MDPI AG
2020-08-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/13/16/3522 |
| _version_ | 1797559298870476800 |
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| author | Su Jeong Lee Jun Hee Lee Jisun Park Wan Doo Kim Su A Park |
| author_facet | Su Jeong Lee Jun Hee Lee Jisun Park Wan Doo Kim Su A Park |
| author_sort | Su Jeong Lee |
| collection | DOAJ |
| description | Recently, many research groups have investigated three-dimensional (3D) bioprinting techniques for tissue engineering and regenerative medicine. The bio-ink used in 3D bioprinting is typically a combination of synthetic and natural materials. In this study, we prepared bio-ink containing porcine skin powder (PSP) to determine rheological properties, biocompatibility, and extracellular matrix (ECM) formation in cells in PSP-ink after 3D printing. PSP was extracted without cells by mechanical, enzymatic, and chemical treatments of porcine dermis tissue. Our developed PSP-containing bio-ink showed enhanced printability and biocompatibility. To identify whether the bio-ink was printable, the viscosity of bio-ink and alginate hydrogel was analyzed with different concentration of PSP. As the PSP concentration increased, viscosity also increased. To assess the biocompatibility of the PSP-containing bio-ink, cells mixed with bio-ink printed structures were measured using a live/dead assay and WST-1 assay. Nearly no dead cells were observed in the structure containing 10 mg/mL PSP-ink, indicating that the amounts of PSP-ink used were nontoxic. In conclusion, the proposed skin dermis decellularized bio-ink is a candidate for 3D bioprinting. |
| first_indexed | 2024-03-10T17:42:20Z |
| format | Article |
| id | doaj.art-ff768264eb0d43e7845689b77d4ef54e |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T17:42:20Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-ff768264eb0d43e7845689b77d4ef54e2023-11-20T09:39:17ZengMDPI AGMaterials1996-19442020-08-011316352210.3390/ma13163522Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue EngineeringSu Jeong Lee0Jun Hee Lee1Jisun Park2Wan Doo Kim3Su A Park4Medical Device Convergence Center, Konyang University Hospital, 158 Gwanjedong-Ro, Seo-Gu, Daejeon 35365, KoreaDepartment of Nature-Inspired Nano Convergence Systems, Nano-Convergence Manufacturing Systems Research Division, Korea Institute of Machinery & Materials (KIMM), 156 Gajeongbuk-Ro, Yuseong-Gu, Daejeon 34103, KoreaMedical Device Development Center, Osong Medical Innovation Foundation, 123 Osongsaengmyung-Ro, Osong-Eub, Heungdeok-Gu, Cheonju-Si, Chungbuk 28160, KoreaDepartment of Nature-Inspired Nano Convergence Systems, Nano-Convergence Manufacturing Systems Research Division, Korea Institute of Machinery & Materials (KIMM), 156 Gajeongbuk-Ro, Yuseong-Gu, Daejeon 34103, KoreaDepartment of Nature-Inspired Nano Convergence Systems, Nano-Convergence Manufacturing Systems Research Division, Korea Institute of Machinery & Materials (KIMM), 156 Gajeongbuk-Ro, Yuseong-Gu, Daejeon 34103, KoreaRecently, many research groups have investigated three-dimensional (3D) bioprinting techniques for tissue engineering and regenerative medicine. The bio-ink used in 3D bioprinting is typically a combination of synthetic and natural materials. In this study, we prepared bio-ink containing porcine skin powder (PSP) to determine rheological properties, biocompatibility, and extracellular matrix (ECM) formation in cells in PSP-ink after 3D printing. PSP was extracted without cells by mechanical, enzymatic, and chemical treatments of porcine dermis tissue. Our developed PSP-containing bio-ink showed enhanced printability and biocompatibility. To identify whether the bio-ink was printable, the viscosity of bio-ink and alginate hydrogel was analyzed with different concentration of PSP. As the PSP concentration increased, viscosity also increased. To assess the biocompatibility of the PSP-containing bio-ink, cells mixed with bio-ink printed structures were measured using a live/dead assay and WST-1 assay. Nearly no dead cells were observed in the structure containing 10 mg/mL PSP-ink, indicating that the amounts of PSP-ink used were nontoxic. In conclusion, the proposed skin dermis decellularized bio-ink is a candidate for 3D bioprinting.https://www.mdpi.com/1996-1944/13/16/35223D bioprintingbio-inkdecellularized extracellular matrixskin dermis |
| spellingShingle | Su Jeong Lee Jun Hee Lee Jisun Park Wan Doo Kim Su A Park Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering Materials 3D bioprinting bio-ink decellularized extracellular matrix skin dermis |
| title | Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering |
| title_full | Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering |
| title_fullStr | Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering |
| title_full_unstemmed | Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering |
| title_short | Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering |
| title_sort | fabrication of 3d printing scaffold with porcine skin decellularized bio ink for soft tissue engineering |
| topic | 3D bioprinting bio-ink decellularized extracellular matrix skin dermis |
| url | https://www.mdpi.com/1996-1944/13/16/3522 |
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