The molecular biological characteristics of Vibrio vulnificus isolated in Guangzhou
ObjectiveTo investigate the molecular and biological characteristics of Vibrio vulnificus （V. vulnificus）isolated from Guangzhou.MethodsThirty-eight strains of V. vulnificus were collected from Guangzhou， and whole-genome sequences were obtained. The population structure of V. vulnificus was inferre...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The Editorial Office of Chinese Journal of Food Hygiene
2023-12-01
|
| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | https://www.zgspws.com/zgspwszz/article/abstract/202312003?st=article_issue |
| _version_ | 1827319101829152768 |
|---|---|
| author | ZHANG Jing CHEN Huiling HE Peng HOU Shuiping BAI Zhijun TAO Xia WU Xinwei |
| author_facet | ZHANG Jing CHEN Huiling HE Peng HOU Shuiping BAI Zhijun TAO Xia WU Xinwei |
| author_sort | ZHANG Jing |
| collection | DOAJ |
| description | ObjectiveTo investigate the molecular and biological characteristics of Vibrio vulnificus （V. vulnificus）isolated from Guangzhou.MethodsThirty-eight strains of V. vulnificus were collected from Guangzhou， and whole-genome sequences were obtained. The population structure of V. vulnificus was inferred by utilizing 50 publicly available genome sequences obtained from NCBI. FineSTRUCTURE software was employed for this analysis. Antibiotic resistance and virulence factors were identified using CARD， ResFinder， and VFDB databases.ResultsFour well-supported phylogenetic groups or lineages （L1-L4） were identified， and all genomes of the strains in Guangzhou were classified into L1 （47%） and L2 （53%）. The predominant ST were ST357， ST157， ST136， ST139， ST345， ST303， and so on， which showed regional aggregation. Multiple genome identification of V. vulnificus revealed 11 drug resistance genes： acrF， CRP， catB9， rpoC， ugd， and others. MCR genes related to polymyxin resistance were identified in V. vulnificus for the first time. The species also carries 23 virulence genes in five classes encoding flagellin， type Ⅱ secretory system protein， capsular polysaccharide， RtxA toxin， iron overload， and other virulence-related genes.ConclusionVibrio vulnificus in Guangzhou undergoes highly homologous recombination and carries a variety of antimicrobial resistance genes and virulence-related genes. Therefore， monitoring and management of Vibrio vulnificus should be strengthened. |
| first_indexed | 2024-04-25T00:12:59Z |
| format | Article |
| id | doaj.art-ff78090fd8cc447f853b3ecddc1624d3 |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-04-25T00:12:59Z |
| publishDate | 2023-12-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-ff78090fd8cc447f853b3ecddc1624d32024-03-13T09:18:29ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562023-12-0135121710171410.13590/j.cjfh.2023.12.0031004-8456（2023）12-1710-05The molecular biological characteristics of Vibrio vulnificus isolated in GuangzhouZHANG Jing0CHEN Huiling1HE Peng2HOU Shuiping3BAI Zhijun4TAO Xia5WU Xinwei6Department of Microbiology Test， Guangzhou Center for Disease Control and Prevention， Guangdong Guangzhou 510440， ChinaDepartment of Microbiology Test， Guangzhou Center for Disease Control and Prevention， Guangdong Guangzhou 510440， ChinaDepartment of Microbiology Test， Guangzhou Center for Disease Control and Prevention， Guangdong Guangzhou 510440， ChinaDepartment of Microbiology Test， Guangzhou Center for Disease Control and Prevention， Guangdong Guangzhou 510440， ChinaDepartment of Microbiology Test， Guangzhou Center for Disease Control and Prevention， Guangdong Guangzhou 510440， ChinaDepartment of Microbiology Test， Guangzhou Center for Disease Control and Prevention， Guangdong Guangzhou 510440， ChinaDepartment of Microbiology Test， Guangzhou Center for Disease Control and Prevention， Guangdong Guangzhou 510440， ChinaObjectiveTo investigate the molecular and biological characteristics of Vibrio vulnificus （V. vulnificus）isolated from Guangzhou.MethodsThirty-eight strains of V. vulnificus were collected from Guangzhou， and whole-genome sequences were obtained. The population structure of V. vulnificus was inferred by utilizing 50 publicly available genome sequences obtained from NCBI. FineSTRUCTURE software was employed for this analysis. Antibiotic resistance and virulence factors were identified using CARD， ResFinder， and VFDB databases.ResultsFour well-supported phylogenetic groups or lineages （L1-L4） were identified， and all genomes of the strains in Guangzhou were classified into L1 （47%） and L2 （53%）. The predominant ST were ST357， ST157， ST136， ST139， ST345， ST303， and so on， which showed regional aggregation. Multiple genome identification of V. vulnificus revealed 11 drug resistance genes： acrF， CRP， catB9， rpoC， ugd， and others. MCR genes related to polymyxin resistance were identified in V. vulnificus for the first time. The species also carries 23 virulence genes in five classes encoding flagellin， type Ⅱ secretory system protein， capsular polysaccharide， RtxA toxin， iron overload， and other virulence-related genes.ConclusionVibrio vulnificus in Guangzhou undergoes highly homologous recombination and carries a variety of antimicrobial resistance genes and virulence-related genes. Therefore， monitoring and management of Vibrio vulnificus should be strengthened.https://www.zgspws.com/zgspwszz/article/abstract/202312003?st=article_issuevibrio vulnificuswhole-genome sequencingpopulation structuremultilocus sequence typingantimicrobial resistance genevirulence-related genes |
| spellingShingle | ZHANG Jing CHEN Huiling HE Peng HOU Shuiping BAI Zhijun TAO Xia WU Xinwei The molecular biological characteristics of Vibrio vulnificus isolated in Guangzhou Zhongguo shipin weisheng zazhi vibrio vulnificus whole-genome sequencing population structure multilocus sequence typing antimicrobial resistance gene virulence-related genes |
| title | The molecular biological characteristics of Vibrio vulnificus isolated in Guangzhou |
| title_full | The molecular biological characteristics of Vibrio vulnificus isolated in Guangzhou |
| title_fullStr | The molecular biological characteristics of Vibrio vulnificus isolated in Guangzhou |
| title_full_unstemmed | The molecular biological characteristics of Vibrio vulnificus isolated in Guangzhou |
| title_short | The molecular biological characteristics of Vibrio vulnificus isolated in Guangzhou |
| title_sort | molecular biological characteristics of vibrio vulnificus isolated in guangzhou |
| topic | vibrio vulnificus whole-genome sequencing population structure multilocus sequence typing antimicrobial resistance gene virulence-related genes |
| url | https://www.zgspws.com/zgspwszz/article/abstract/202312003?st=article_issue |
| work_keys_str_mv | AT zhangjing themolecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT chenhuiling themolecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT hepeng themolecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT houshuiping themolecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT baizhijun themolecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT taoxia themolecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT wuxinwei themolecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT zhangjing molecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT chenhuiling molecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT hepeng molecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT houshuiping molecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT baizhijun molecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT taoxia molecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou AT wuxinwei molecularbiologicalcharacteristicsofvibriovulnificusisolatedinguangzhou |