Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in Austria
In recent years, antibiotic-resistant bacteria with an impact on human health, such as extended spectrum β-lactamase (ESBL)-containing <i>Enterobacteriaceae</i>, methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and vancomycin-resistant Enterococci (VRE), have become...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/4/466 |
| _version_ | 1797537094295355392 |
|---|---|
| author | Herbert Galler Josefa Luxner Christian Petternel Franz F. Reinthaler Juliana Habib Doris Haas Clemens Kittinger Peter Pless Gebhard Feierl Gernot Zarfel |
| author_facet | Herbert Galler Josefa Luxner Christian Petternel Franz F. Reinthaler Juliana Habib Doris Haas Clemens Kittinger Peter Pless Gebhard Feierl Gernot Zarfel |
| author_sort | Herbert Galler |
| collection | DOAJ |
| description | In recent years, antibiotic-resistant bacteria with an impact on human health, such as extended spectrum β-lactamase (ESBL)-containing <i>Enterobacteriaceae</i>, methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and vancomycin-resistant Enterococci (VRE), have become more common in food. This is due to the use of antibiotics in animal husbandry, which leads to the promotion of antibiotic resistance and thus also makes food a source of such resistant bacteria. Most studies dealing with this issue usually focus on the animals or processed food products to examine the antibiotic resistant bacteria. This study investigated the intestine as another main habitat besides the skin for multiresistant bacteria. For this purpose, faeces samples were taken directly from the intestines of swine (<i>n</i> = 71) and broiler (<i>n</i> = 100) during the slaughter process and analysed. All samples were from animals fed in Austria and slaughtered in Austrian slaughterhouses for food production. The samples were examined for the presence of ESBL-producing Enterobacteriaceae, MRSA, MRCoNS and VRE. The resistance genes of the isolated bacteria were detected and sequenced by PCR. Phenotypic ESBL-producing <i>Escherichia coli</i> could be isolated in 10% of broiler casings (10 out of 100) and 43.6% of swine casings (31 out of 71). In line with previous studies, the results of this study showed that CTX-M-1 was the dominant ESBL produced by <i>E. coli</i> from swine (<i>n</i> = 25, 83.3%) and SHV-12 from broilers (<i>n</i> = 13, 81.3%). Overall, the frequency of positive samples with multidrug-resistant bacteria was lower than in most comparable studies focusing on meat products. |
| first_indexed | 2024-03-10T12:11:08Z |
| format | Article |
| id | doaj.art-ff813360394e429fb4ad45f38eb12e74 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T12:11:08Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-ff813360394e429fb4ad45f38eb12e742023-11-21T16:16:06ZengMDPI AGAntibiotics2079-63822021-04-0110446610.3390/antibiotics10040466Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in AustriaHerbert Galler0Josefa Luxner1Christian Petternel2Franz F. Reinthaler3Juliana Habib4Doris Haas5Clemens Kittinger6Peter Pless7Gebhard Feierl8Gernot Zarfel9D&R Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaD&R Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaInstitute of Laboratory Diagnostics and Microbiology, Klinikum-Klagenfurt am Wörthersee, Feschnigstraße 11, 9020 Klagenfurt, AustriaD&R Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaD&R Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaD&R Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaD&R Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaAnimal Health Service of the Department of Veterinary Administration, Styrian Government, Friedrichgasse 9, 8010 Graz, AustriaD&R Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaD&R Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Neue Stiftingtalstraße 6, 8010 Graz, AustriaIn recent years, antibiotic-resistant bacteria with an impact on human health, such as extended spectrum β-lactamase (ESBL)-containing <i>Enterobacteriaceae</i>, methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and vancomycin-resistant Enterococci (VRE), have become more common in food. This is due to the use of antibiotics in animal husbandry, which leads to the promotion of antibiotic resistance and thus also makes food a source of such resistant bacteria. Most studies dealing with this issue usually focus on the animals or processed food products to examine the antibiotic resistant bacteria. This study investigated the intestine as another main habitat besides the skin for multiresistant bacteria. For this purpose, faeces samples were taken directly from the intestines of swine (<i>n</i> = 71) and broiler (<i>n</i> = 100) during the slaughter process and analysed. All samples were from animals fed in Austria and slaughtered in Austrian slaughterhouses for food production. The samples were examined for the presence of ESBL-producing Enterobacteriaceae, MRSA, MRCoNS and VRE. The resistance genes of the isolated bacteria were detected and sequenced by PCR. Phenotypic ESBL-producing <i>Escherichia coli</i> could be isolated in 10% of broiler casings (10 out of 100) and 43.6% of swine casings (31 out of 71). In line with previous studies, the results of this study showed that CTX-M-1 was the dominant ESBL produced by <i>E. coli</i> from swine (<i>n</i> = 25, 83.3%) and SHV-12 from broilers (<i>n</i> = 13, 81.3%). Overall, the frequency of positive samples with multidrug-resistant bacteria was lower than in most comparable studies focusing on meat products.https://www.mdpi.com/2079-6382/10/4/466broilerswineESBLVRECTX-MSHV |
| spellingShingle | Herbert Galler Josefa Luxner Christian Petternel Franz F. Reinthaler Juliana Habib Doris Haas Clemens Kittinger Peter Pless Gebhard Feierl Gernot Zarfel Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in Austria Antibiotics broiler swine ESBL VRE CTX-M SHV |
| title | Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in Austria |
| title_full | Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in Austria |
| title_fullStr | Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in Austria |
| title_full_unstemmed | Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in Austria |
| title_short | Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in Austria |
| title_sort | multiresistant bacteria isolated from intestinal faeces of farm animals in austria |
| topic | broiler swine ESBL VRE CTX-M SHV |
| url | https://www.mdpi.com/2079-6382/10/4/466 |
| work_keys_str_mv | AT herbertgaller multiresistantbacteriaisolatedfromintestinalfaecesoffarmanimalsinaustria AT josefaluxner multiresistantbacteriaisolatedfromintestinalfaecesoffarmanimalsinaustria AT christianpetternel multiresistantbacteriaisolatedfromintestinalfaecesoffarmanimalsinaustria AT franzfreinthaler multiresistantbacteriaisolatedfromintestinalfaecesoffarmanimalsinaustria AT julianahabib multiresistantbacteriaisolatedfromintestinalfaecesoffarmanimalsinaustria AT dorishaas multiresistantbacteriaisolatedfromintestinalfaecesoffarmanimalsinaustria AT clemenskittinger multiresistantbacteriaisolatedfromintestinalfaecesoffarmanimalsinaustria AT peterpless multiresistantbacteriaisolatedfromintestinalfaecesoffarmanimalsinaustria AT gebhardfeierl multiresistantbacteriaisolatedfromintestinalfaecesoffarmanimalsinaustria AT gernotzarfel multiresistantbacteriaisolatedfromintestinalfaecesoffarmanimalsinaustria |