Beneficial Effects of Hyaluronan-Based Hydrogel Implantation after Cortical Traumatic Injury
Traumatic brain injury (TBI) causes cell death mainly in the cerebral cortex. We have previously reported that transplantation of embryonic cortical neurons immediately after cortical injury allows the anatomical reconstruction of injured pathways and that a delay between cortical injury and cell tr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/23/3831 |
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| author | Anaïs Lainé Sébastien Brot Afsaneh Gaillard |
| author_facet | Anaïs Lainé Sébastien Brot Afsaneh Gaillard |
| author_sort | Anaïs Lainé |
| collection | DOAJ |
| description | Traumatic brain injury (TBI) causes cell death mainly in the cerebral cortex. We have previously reported that transplantation of embryonic cortical neurons immediately after cortical injury allows the anatomical reconstruction of injured pathways and that a delay between cortical injury and cell transplantation can partially improve transplantation efficiency. Biomaterials supporting repair processes in combination with cell transplantation are in development. Hyaluronic acid (HA) hydrogel has attracted increasing interest in the field of tissue engineering due to its attractive biological properties. However, before combining the cell with the HA hydrogel for transplantation, it is important to know the effects of the implanted hydrogel alone. Here, we investigated the therapeutic effect of HA on host tissue after a cortical trauma. For this, we implanted HA hydrogel into the lesioned motor cortex of adult mice immediately or one week after a lesion. Our results show the vascularization of the implanted hydrogel. At one month after HA implantation, we observed a reduction in the glial scar around the lesion and the presence of the newly generated oligodendrocytes, immature and mature neurons within the hydrogel. Implanted hydrogel provides favorable environments for the survival and maturation of the newly generated neurons. Collectively, these results suggest a beneficial effect of biomaterial after a cortical traumatic injury. |
| first_indexed | 2024-03-09T17:51:54Z |
| format | Article |
| id | doaj.art-ff82b2c5ff05435cb8ecd2301aecc41d |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T17:51:54Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-ff82b2c5ff05435cb8ecd2301aecc41d2023-11-24T10:44:33ZengMDPI AGCells2073-44092022-11-011123383110.3390/cells11233831Beneficial Effects of Hyaluronan-Based Hydrogel Implantation after Cortical Traumatic InjuryAnaïs Lainé0Sébastien Brot1Afsaneh Gaillard2Laboratory of Experimental and Clinical Neurosciences, INSERM U-1084, University of Poitiers, CEDEX 09, 86073 Poitiers, FranceLaboratory of Experimental and Clinical Neurosciences, INSERM U-1084, University of Poitiers, CEDEX 09, 86073 Poitiers, FranceLaboratory of Experimental and Clinical Neurosciences, INSERM U-1084, University of Poitiers, CEDEX 09, 86073 Poitiers, FranceTraumatic brain injury (TBI) causes cell death mainly in the cerebral cortex. We have previously reported that transplantation of embryonic cortical neurons immediately after cortical injury allows the anatomical reconstruction of injured pathways and that a delay between cortical injury and cell transplantation can partially improve transplantation efficiency. Biomaterials supporting repair processes in combination with cell transplantation are in development. Hyaluronic acid (HA) hydrogel has attracted increasing interest in the field of tissue engineering due to its attractive biological properties. However, before combining the cell with the HA hydrogel for transplantation, it is important to know the effects of the implanted hydrogel alone. Here, we investigated the therapeutic effect of HA on host tissue after a cortical trauma. For this, we implanted HA hydrogel into the lesioned motor cortex of adult mice immediately or one week after a lesion. Our results show the vascularization of the implanted hydrogel. At one month after HA implantation, we observed a reduction in the glial scar around the lesion and the presence of the newly generated oligodendrocytes, immature and mature neurons within the hydrogel. Implanted hydrogel provides favorable environments for the survival and maturation of the newly generated neurons. Collectively, these results suggest a beneficial effect of biomaterial after a cortical traumatic injury.https://www.mdpi.com/2073-4409/11/23/3831biomaterialhyaluronantraumatic brain injuryneuroinflammation |
| spellingShingle | Anaïs Lainé Sébastien Brot Afsaneh Gaillard Beneficial Effects of Hyaluronan-Based Hydrogel Implantation after Cortical Traumatic Injury Cells biomaterial hyaluronan traumatic brain injury neuroinflammation |
| title | Beneficial Effects of Hyaluronan-Based Hydrogel Implantation after Cortical Traumatic Injury |
| title_full | Beneficial Effects of Hyaluronan-Based Hydrogel Implantation after Cortical Traumatic Injury |
| title_fullStr | Beneficial Effects of Hyaluronan-Based Hydrogel Implantation after Cortical Traumatic Injury |
| title_full_unstemmed | Beneficial Effects of Hyaluronan-Based Hydrogel Implantation after Cortical Traumatic Injury |
| title_short | Beneficial Effects of Hyaluronan-Based Hydrogel Implantation after Cortical Traumatic Injury |
| title_sort | beneficial effects of hyaluronan based hydrogel implantation after cortical traumatic injury |
| topic | biomaterial hyaluronan traumatic brain injury neuroinflammation |
| url | https://www.mdpi.com/2073-4409/11/23/3831 |
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