Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive rats
OBJECTIVES: This study was conducted to determine whether the blood pressure-lowering effect of Nigella sativa might be mediated by its effects on nitric oxide, angiotensin-converting enzyme, heme oxygenase and oxidative stress markers. METHODS: Twenty-four adult male Sprague-Dawley rats were divid...
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| Format: | Article |
| Language: | English |
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Elsevier España
2015-11-01
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| Series: | Clinics |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322015001100751&lng=en&tlng=en |
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| author | Kamsiah Jaarin Wai Dic Foong Min Hui Yeoh Zaman Yusoff Nik Kamarul Haji Mohd Saad Qodriyah Abdullah Azman Japar Sidik Fadhlullah Zuhair Abdul Hamid Juliana Yusof Kamisah |
| author_facet | Kamsiah Jaarin Wai Dic Foong Min Hui Yeoh Zaman Yusoff Nik Kamarul Haji Mohd Saad Qodriyah Abdullah Azman Japar Sidik Fadhlullah Zuhair Abdul Hamid Juliana Yusof Kamisah |
| author_sort | Kamsiah Jaarin |
| collection | DOAJ |
| description | OBJECTIVES: This study was conducted to determine whether the blood pressure-lowering effect of Nigella sativa might be mediated by its effects on nitric oxide, angiotensin-converting enzyme, heme oxygenase and oxidative stress markers. METHODS: Twenty-four adult male Sprague-Dawley rats were divided equally into 4 groups. One group served as the control (group 1), whereas the other three groups (groups 2-4) were administered L-NAME (25 mg/kg, intraperitoneally). Groups 3 and 4 were given oral nicardipine daily at a dose of 3 mg/kg and Nigella sativa oil at a dose of 2.5 mg/kg for 8 weeks, respectively, concomitantly with L-NAME administration. RESULTS: Nigella sativa oil prevented the increase in systolic blood pressure in the L-NAME-treated rats. The blood pressure reduction was associated with a reduction in cardiac lipid peroxidation product, NADPH oxidase, angiotensin-converting enzyme activity and plasma nitric oxide, as well as with an increase in heme oxygenase-1 activity in the heart. The effects of Nigella sativa on blood pressure, lipid peroxidation product, nicotinamide adenine dinucleotide phosphate oxidase and angiotensin-converting enzyme were similar to those of nicardipine. In contrast, L-NAME had opposite effects on lipid peroxidation, angiotensin-converting enzyme and NO. CONCLUSION: The antihypertensive effect of Nigella sativa oil appears to be mediated by a reduction in cardiac oxidative stress and angiotensin-converting enzyme activity, an increase in cardiac heme oxygenase-1 activity and a prevention of plasma nitric oxide loss. Thus, Nigella sativa oil might be beneficial for controlling hypertension. |
| first_indexed | 2024-04-12T09:59:17Z |
| format | Article |
| id | doaj.art-ff82c2da49384924be50b4233e8c51c2 |
| institution | Directory Open Access Journal |
| issn | 1980-5322 |
| language | English |
| last_indexed | 2024-04-12T09:59:17Z |
| publishDate | 2015-11-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-ff82c2da49384924be50b4233e8c51c22022-12-22T03:37:37ZengElsevier EspañaClinics1980-53222015-11-01701175175710.6061/clinics/2015(11)07S1807-59322015001100751Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive ratsKamsiah JaarinWai Dic FoongMin Hui YeohZaman Yusoff Nik KamarulHaji Mohd Saad QodriyahAbdullah AzmanJapar Sidik Fadhlullah ZuhairAbdul Hamid JulianaYusof KamisahOBJECTIVES: This study was conducted to determine whether the blood pressure-lowering effect of Nigella sativa might be mediated by its effects on nitric oxide, angiotensin-converting enzyme, heme oxygenase and oxidative stress markers. METHODS: Twenty-four adult male Sprague-Dawley rats were divided equally into 4 groups. One group served as the control (group 1), whereas the other three groups (groups 2-4) were administered L-NAME (25 mg/kg, intraperitoneally). Groups 3 and 4 were given oral nicardipine daily at a dose of 3 mg/kg and Nigella sativa oil at a dose of 2.5 mg/kg for 8 weeks, respectively, concomitantly with L-NAME administration. RESULTS: Nigella sativa oil prevented the increase in systolic blood pressure in the L-NAME-treated rats. The blood pressure reduction was associated with a reduction in cardiac lipid peroxidation product, NADPH oxidase, angiotensin-converting enzyme activity and plasma nitric oxide, as well as with an increase in heme oxygenase-1 activity in the heart. The effects of Nigella sativa on blood pressure, lipid peroxidation product, nicotinamide adenine dinucleotide phosphate oxidase and angiotensin-converting enzyme were similar to those of nicardipine. In contrast, L-NAME had opposite effects on lipid peroxidation, angiotensin-converting enzyme and NO. CONCLUSION: The antihypertensive effect of Nigella sativa oil appears to be mediated by a reduction in cardiac oxidative stress and angiotensin-converting enzyme activity, an increase in cardiac heme oxygenase-1 activity and a prevention of plasma nitric oxide loss. Thus, Nigella sativa oil might be beneficial for controlling hypertension.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322015001100751&lng=en&tlng=enNigella sativaAntihypertensiveAngiotensin-converting EnzymeHeme OxygenaseNADPH Oxidase |
| spellingShingle | Kamsiah Jaarin Wai Dic Foong Min Hui Yeoh Zaman Yusoff Nik Kamarul Haji Mohd Saad Qodriyah Abdullah Azman Japar Sidik Fadhlullah Zuhair Abdul Hamid Juliana Yusof Kamisah Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive rats Clinics Nigella sativa Antihypertensive Angiotensin-converting Enzyme Heme Oxygenase NADPH Oxidase |
| title | Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive rats |
| title_full | Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive rats |
| title_fullStr | Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive rats |
| title_full_unstemmed | Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive rats |
| title_short | Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive rats |
| title_sort | mechanisms of the antihypertensive effects of nigella sativa oil in l name induced hypertensive rats |
| topic | Nigella sativa Antihypertensive Angiotensin-converting Enzyme Heme Oxygenase NADPH Oxidase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322015001100751&lng=en&tlng=en |
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