Characterization of Beta-Lactam Resistome of <i>Escherichia coli</i> Causing Nosocomial Infections
Nosocomial infections caused by <i>Escherichia coli</i> pose significant therapeutic challenges due to the high expression of genes encoding antimicrobial drug resistance. In this study, we investigated the conformation of the beta-lactam resistome responsible for the specific pattern of...
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MDPI AG
2023-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/9/1355 |
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| author | Rosalino Vázquez-López Tanya Hernández-Martínez Selene Ivonne Larios-Fernández Celia Piña-Leyva Manuel Lara-Lozano Tayde Guerrero-González Javier Martínez-Bautista Eduardo Gómez-Conde Juan Antonio González-Barrios |
| author_facet | Rosalino Vázquez-López Tanya Hernández-Martínez Selene Ivonne Larios-Fernández Celia Piña-Leyva Manuel Lara-Lozano Tayde Guerrero-González Javier Martínez-Bautista Eduardo Gómez-Conde Juan Antonio González-Barrios |
| author_sort | Rosalino Vázquez-López |
| collection | DOAJ |
| description | Nosocomial infections caused by <i>Escherichia coli</i> pose significant therapeutic challenges due to the high expression of genes encoding antimicrobial drug resistance. In this study, we investigated the conformation of the beta-lactam resistome responsible for the specific pattern of resistance against beta-lactam antibiotics. A total of 218 <i>Escherichia coli</i> strains were isolated from in-hospital patients diagnosed with nosocomial infections, obtained from various sources such as urine (<i>n</i> = 49, 22.48%), vaginal discharge (<i>n</i> = 46, 21.10%), catheter tips (<i>n</i> = 14, 6.42%), blood (<i>n</i> = 13, 5.96%), feces (<i>n</i> = 12, 5.50%), sputum (<i>n</i> = 11, 5.05%), biopsies (<i>n</i> = 8, 3.67%), cerebrospinal fluid (<i>n</i> = 2, 0.92%) and other unspecified discharges (<i>n</i> = 63, 28.90%). To characterize the beta-lactam resistome, all strains were subjected to antibiotic dilution tests and grown in beta-lactam antibiotics supplemented with Luria culture medium. Subsequently, multiplex PCR and next-generation sequencing were conducted. The results show a multi-drug-resistance phenotype, particularly against beta-lactam drugs. The primary determinant of this resistance was the expression of the blaTEM gene family, with 209 positive strains (95.87%) expressing it as a single gene (<i>n</i> = 47, 21.6%) or in combination with other genes. Common combinations included <i>bla</i>TEM + <i>bla</i>CTX (<i>n</i> = 42, 19.3%), <i>bla</i>TEM + <i>bla</i>CTX + <i>bla</i>SHV (<i>n</i> = 13, 6%) and <i>bla</i>TEM + <i>bla</i>CTX + <i>bla</i>BIL (<i>n</i> = 12, 5.5%), among others. The beta-lactam resistome of nosocomial <i>Escherichia coli</i> strains isolated from inpatients at the “October first” Regional Hospital of ISSSTE was predominantly composed of members of the blaTEM gene family, expressed in various configurations along with different members of other beta-lactamase gene families. |
| first_indexed | 2024-03-10T23:07:02Z |
| format | Article |
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| last_indexed | 2024-03-10T23:07:02Z |
| publishDate | 2023-08-01 |
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| series | Antibiotics |
| spelling | doaj.art-ff844160a6554f649ffe4fef2b11e7de2023-11-19T09:16:37ZengMDPI AGAntibiotics2079-63822023-08-01129135510.3390/antibiotics12091355Characterization of Beta-Lactam Resistome of <i>Escherichia coli</i> Causing Nosocomial InfectionsRosalino Vázquez-López0Tanya Hernández-Martínez1Selene Ivonne Larios-Fernández2Celia Piña-Leyva3Manuel Lara-Lozano4Tayde Guerrero-González5Javier Martínez-Bautista6Eduardo Gómez-Conde7Juan Antonio González-Barrios8Departamento de Microbiología, Centro de Investigación en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud Universidad Anáhuac México Norte, Huixquilucan 52786, MexicoLaboratorio de Medicina Genómica, Hospital Regional “Primero de Octubre”, ISSSTE, Av. Instituto Politécnico Nacional 1669, Lindavista, Gustavo A. Madero, Ciudad de México 07300, MexicoLaboratorio de Medicina Genómica, Hospital Regional “Primero de Octubre”, ISSSTE, Av. Instituto Politécnico Nacional 1669, Lindavista, Gustavo A. Madero, Ciudad de México 07300, MexicoLaboratorio de Medicina Genómica, Hospital Regional “Primero de Octubre”, ISSSTE, Av. Instituto Politécnico Nacional 1669, Lindavista, Gustavo A. Madero, Ciudad de México 07300, MexicoLaboratorio de Medicina Genómica, Hospital Regional “Primero de Octubre”, ISSSTE, Av. Instituto Politécnico Nacional 1669, Lindavista, Gustavo A. Madero, Ciudad de México 07300, MexicoLaboratorio de Medicina Genómica, Hospital Regional “Primero de Octubre”, ISSSTE, Av. Instituto Politécnico Nacional 1669, Lindavista, Gustavo A. Madero, Ciudad de México 07300, MexicoLaboratorio de Microbiología, Hospital Regional “Primero de Octubre”, ISSSTE, Av. Instituto Politécnico Nacional 1669, Lindavista, Gustavo A. Madero, Ciudad de México 07300, MexicoDepartamento de Inmunobiología, Facultad de Medicina, Benemérita Universidad Autónoma de Puebla (BUAP), Puebla 72420, MexicoLaboratorio de Medicina Genómica, Hospital Regional “Primero de Octubre”, ISSSTE, Av. Instituto Politécnico Nacional 1669, Lindavista, Gustavo A. Madero, Ciudad de México 07300, MexicoNosocomial infections caused by <i>Escherichia coli</i> pose significant therapeutic challenges due to the high expression of genes encoding antimicrobial drug resistance. In this study, we investigated the conformation of the beta-lactam resistome responsible for the specific pattern of resistance against beta-lactam antibiotics. A total of 218 <i>Escherichia coli</i> strains were isolated from in-hospital patients diagnosed with nosocomial infections, obtained from various sources such as urine (<i>n</i> = 49, 22.48%), vaginal discharge (<i>n</i> = 46, 21.10%), catheter tips (<i>n</i> = 14, 6.42%), blood (<i>n</i> = 13, 5.96%), feces (<i>n</i> = 12, 5.50%), sputum (<i>n</i> = 11, 5.05%), biopsies (<i>n</i> = 8, 3.67%), cerebrospinal fluid (<i>n</i> = 2, 0.92%) and other unspecified discharges (<i>n</i> = 63, 28.90%). To characterize the beta-lactam resistome, all strains were subjected to antibiotic dilution tests and grown in beta-lactam antibiotics supplemented with Luria culture medium. Subsequently, multiplex PCR and next-generation sequencing were conducted. The results show a multi-drug-resistance phenotype, particularly against beta-lactam drugs. The primary determinant of this resistance was the expression of the blaTEM gene family, with 209 positive strains (95.87%) expressing it as a single gene (<i>n</i> = 47, 21.6%) or in combination with other genes. Common combinations included <i>bla</i>TEM + <i>bla</i>CTX (<i>n</i> = 42, 19.3%), <i>bla</i>TEM + <i>bla</i>CTX + <i>bla</i>SHV (<i>n</i> = 13, 6%) and <i>bla</i>TEM + <i>bla</i>CTX + <i>bla</i>BIL (<i>n</i> = 12, 5.5%), among others. The beta-lactam resistome of nosocomial <i>Escherichia coli</i> strains isolated from inpatients at the “October first” Regional Hospital of ISSSTE was predominantly composed of members of the blaTEM gene family, expressed in various configurations along with different members of other beta-lactamase gene families.https://www.mdpi.com/2079-6382/12/9/1355<i>Escherichia coli</i>beta-lactam resistomeantibiotic multi-resistancepathotypes |
| spellingShingle | Rosalino Vázquez-López Tanya Hernández-Martínez Selene Ivonne Larios-Fernández Celia Piña-Leyva Manuel Lara-Lozano Tayde Guerrero-González Javier Martínez-Bautista Eduardo Gómez-Conde Juan Antonio González-Barrios Characterization of Beta-Lactam Resistome of <i>Escherichia coli</i> Causing Nosocomial Infections Antibiotics <i>Escherichia coli</i> beta-lactam resistome antibiotic multi-resistance pathotypes |
| title | Characterization of Beta-Lactam Resistome of <i>Escherichia coli</i> Causing Nosocomial Infections |
| title_full | Characterization of Beta-Lactam Resistome of <i>Escherichia coli</i> Causing Nosocomial Infections |
| title_fullStr | Characterization of Beta-Lactam Resistome of <i>Escherichia coli</i> Causing Nosocomial Infections |
| title_full_unstemmed | Characterization of Beta-Lactam Resistome of <i>Escherichia coli</i> Causing Nosocomial Infections |
| title_short | Characterization of Beta-Lactam Resistome of <i>Escherichia coli</i> Causing Nosocomial Infections |
| title_sort | characterization of beta lactam resistome of i escherichia coli i causing nosocomial infections |
| topic | <i>Escherichia coli</i> beta-lactam resistome antibiotic multi-resistance pathotypes |
| url | https://www.mdpi.com/2079-6382/12/9/1355 |
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