Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide
Katsutoshi Kokubun,1,2 Sachiko Matsumura,1 Masako Yudasaka,3,4 Sumio Iijima,3,4 Kiyotaka Shiba1 1Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan; 2Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan; 3Nanomaterials Rese...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2018-03-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/immobilization-of-a-carbon-nanomaterial-based-localized-drug-release-s-peer-reviewed-article-IJN |
| _version_ | 1818432449522171904 |
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| author | Kokubun K Matsumura S Yudasaka M Iijima S Shiba K |
| author_facet | Kokubun K Matsumura S Yudasaka M Iijima S Shiba K |
| author_sort | Kokubun K |
| collection | DOAJ |
| description | Katsutoshi Kokubun,1,2 Sachiko Matsumura,1 Masako Yudasaka,3,4 Sumio Iijima,3,4 Kiyotaka Shiba1 1Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan; 2Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan; 3Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan; 4Graduate School of Science and Technology, Meijo University, Nagoya, Japan Introduction: Inorganic materials are widely used in medical devices, such as artificial hearts, vessels, and joints, in stents, and as nanocarriers for drug-delivery systems. Carbon nanomaterials are of particular interest due to their biological inertness and their capability to accommodate molecules. Several attempts have been proposed, in which carbon nanomaterials are used as nanocarriers for the systemic delivery of drugs.Materials and methods: We developed a drug-delivery system in which oxidized single-walled carbon nanohorns (oxSWNHs) were immobilized on a titanium (Ti) surface using material-binding peptides to enable localized drug delivery. For this purpose, we utilized a bispecific peptidic aptamer comprising a core sequence of a Ti-binding peptide and a SWNH-binding peptide to immobilize oxSWNHs on Ti.Results: Scanning electron microscopy was used to confirm the presence of oxSWNHs adsorbed onto the Ti surface, and a quartz crystal microbalance was used to evaluate the binding process during oxSWNH adsorption. The oxSWNHs-ornamented Ti substrate was nontoxic to cells and released biologically active dexamethasone over a sustained period.Conclusion: This oxSWNHs-immobilized system can be used to modify the surface of Ti in implants and be loaded with drugs that stimulate osteogenesis and bone regeneration. Keywords: drug carrier, drug delivery, carbon nanomaterial, carbon nanohorn, peptide aptamer |
| first_indexed | 2024-12-14T16:05:23Z |
| format | Article |
| id | doaj.art-ff858d7220114030801e151a0a52420e |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-14T16:05:23Z |
| publishDate | 2018-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-ff858d7220114030801e151a0a52420e2022-12-21T22:55:06ZengDove Medical PressInternational Journal of Nanomedicine1178-20132018-03-01Volume 131643165237298Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptideKokubun KMatsumura SYudasaka MIijima SShiba KKatsutoshi Kokubun,1,2 Sachiko Matsumura,1 Masako Yudasaka,3,4 Sumio Iijima,3,4 Kiyotaka Shiba1 1Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan; 2Department of Clinical Pathophysiology, Tokyo Dental College, Tokyo, Japan; 3Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan; 4Graduate School of Science and Technology, Meijo University, Nagoya, Japan Introduction: Inorganic materials are widely used in medical devices, such as artificial hearts, vessels, and joints, in stents, and as nanocarriers for drug-delivery systems. Carbon nanomaterials are of particular interest due to their biological inertness and their capability to accommodate molecules. Several attempts have been proposed, in which carbon nanomaterials are used as nanocarriers for the systemic delivery of drugs.Materials and methods: We developed a drug-delivery system in which oxidized single-walled carbon nanohorns (oxSWNHs) were immobilized on a titanium (Ti) surface using material-binding peptides to enable localized drug delivery. For this purpose, we utilized a bispecific peptidic aptamer comprising a core sequence of a Ti-binding peptide and a SWNH-binding peptide to immobilize oxSWNHs on Ti.Results: Scanning electron microscopy was used to confirm the presence of oxSWNHs adsorbed onto the Ti surface, and a quartz crystal microbalance was used to evaluate the binding process during oxSWNH adsorption. The oxSWNHs-ornamented Ti substrate was nontoxic to cells and released biologically active dexamethasone over a sustained period.Conclusion: This oxSWNHs-immobilized system can be used to modify the surface of Ti in implants and be loaded with drugs that stimulate osteogenesis and bone regeneration. Keywords: drug carrier, drug delivery, carbon nanomaterial, carbon nanohorn, peptide aptamerhttps://www.dovepress.com/immobilization-of-a-carbon-nanomaterial-based-localized-drug-release-s-peer-reviewed-article-IJNdrug carrierdrug deliverycarbon nanomaterialcarbon nanohornpeptide aptamer |
| spellingShingle | Kokubun K Matsumura S Yudasaka M Iijima S Shiba K Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide International Journal of Nanomedicine drug carrier drug delivery carbon nanomaterial carbon nanohorn peptide aptamer |
| title | Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide |
| title_full | Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide |
| title_fullStr | Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide |
| title_full_unstemmed | Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide |
| title_short | Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide |
| title_sort | immobilization of a carbon nanomaterial based localized drug release system using a bispecific material binding peptide |
| topic | drug carrier drug delivery carbon nanomaterial carbon nanohorn peptide aptamer |
| url | https://www.dovepress.com/immobilization-of-a-carbon-nanomaterial-based-localized-drug-release-s-peer-reviewed-article-IJN |
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