Cold-induced ginsenosides accumulation is associated with the alteration in DNA methylation and relative gene expression in perennial American ginseng (Panax quinquefolius L.) along with its plant growth and development process
Background: Ginsenosides accumulation responses to temperature are critical to quality formation in cold-dependent American ginseng. However, the studies on cold requirement mechanism relevant to ginsenosides have been limited in this species. Methods: Two experiments were carried out: one was a mul...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Journal of Ginseng Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845319300272 |
| _version_ | 1819016017473437696 |
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| author | Mengzhen Hao Yuhang Zhou Jinhui Zhou Min Zhang Kangjiao Yan Sheng Jiang Wenshui Wang Xiaoping Peng San Zhou |
| author_facet | Mengzhen Hao Yuhang Zhou Jinhui Zhou Min Zhang Kangjiao Yan Sheng Jiang Wenshui Wang Xiaoping Peng San Zhou |
| author_sort | Mengzhen Hao |
| collection | DOAJ |
| description | Background: Ginsenosides accumulation responses to temperature are critical to quality formation in cold-dependent American ginseng. However, the studies on cold requirement mechanism relevant to ginsenosides have been limited in this species. Methods: Two experiments were carried out: one was a multivariate linear regression analysis between the ginsenosides accumulation and the environmental conditions of American ginseng from different sites of China and the other was a synchronous determination of ginsenosides accumulation, overall DNA methylation, and relative gene expression in different tissues during different developmental stages of American ginseng after experiencing different cold exposure duration treatments. Results: Results showed that the variation of the contents as well as the yields of total and individual ginsenosides Rg1, Re, and Rb1 in the roots were closely associated with environmental temperature conditions which implied that the cold environment plays a decisive role in the ginsenoside accumulation of American ginseng. Further results showed that there is a cyclically reversible dynamism between methylation and demethylation of DNA in the perennial American ginseng in response to temperature seasonality. And sufficient cold exposure duration in winter caused sufficient DNA demethylation in tender leaves in early spring and then accompanied the high expression of flowering gene PqFT in flowering stages and ginsenosides biosynthesis gene PqDDS in green berry stages successively, and finally, maximum ginsenosides accumulation occurred in the roots of American ginseng. Conclusion: We, therefore, hypothesized that cold-induced DNA methylation changes might regulate relative gene expression involving both plant development and plant secondary metabolites in such cold-dependent perennial plant species. |
| first_indexed | 2024-12-21T02:40:56Z |
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| id | doaj.art-ff86f49fd2584daca51672f9761ab6e1 |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-12-21T02:40:56Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-ff86f49fd2584daca51672f9761ab6e12022-12-21T19:18:41ZengElsevierJournal of Ginseng Research1226-84532020-09-01445747755Cold-induced ginsenosides accumulation is associated with the alteration in DNA methylation and relative gene expression in perennial American ginseng (Panax quinquefolius L.) along with its plant growth and development processMengzhen Hao0Yuhang Zhou1Jinhui Zhou2Min Zhang3Kangjiao Yan4Sheng Jiang5Wenshui Wang6Xiaoping Peng7San Zhou8Department of Pharmcognosy, School of Pharmacy, Qingdao University, Qingdao, ChinaFaculty of Medicine, Tottori University, Tottori, JapanDepartment of Pharmcognosy, School of Pharmacy, Qingdao University, Qingdao, ChinaDepartment of Pharmcognosy, School of Pharmacy, Qingdao University, Qingdao, ChinaDepartment of Pharmcognosy, School of Pharmacy, Qingdao University, Qingdao, ChinaShandong Wendeng Jizhen Ginseng Industry Co., Ltd., Weihai, ChinaShandong Wendeng Jizhen Ginseng Industry Co., Ltd., Weihai, ChinaDepartment of Pharmcognosy, School of Pharmacy, Qingdao University, Qingdao, ChinaDepartment of Pharmcognosy, School of Pharmacy, Qingdao University, Qingdao, China; Corresponding author. Department of Pharmcognosy, School of Pharmacy, Qingdao University, #38 Dengzhou Road, North District, Qingdao City, Shandong Province 266021, China.Background: Ginsenosides accumulation responses to temperature are critical to quality formation in cold-dependent American ginseng. However, the studies on cold requirement mechanism relevant to ginsenosides have been limited in this species. Methods: Two experiments were carried out: one was a multivariate linear regression analysis between the ginsenosides accumulation and the environmental conditions of American ginseng from different sites of China and the other was a synchronous determination of ginsenosides accumulation, overall DNA methylation, and relative gene expression in different tissues during different developmental stages of American ginseng after experiencing different cold exposure duration treatments. Results: Results showed that the variation of the contents as well as the yields of total and individual ginsenosides Rg1, Re, and Rb1 in the roots were closely associated with environmental temperature conditions which implied that the cold environment plays a decisive role in the ginsenoside accumulation of American ginseng. Further results showed that there is a cyclically reversible dynamism between methylation and demethylation of DNA in the perennial American ginseng in response to temperature seasonality. And sufficient cold exposure duration in winter caused sufficient DNA demethylation in tender leaves in early spring and then accompanied the high expression of flowering gene PqFT in flowering stages and ginsenosides biosynthesis gene PqDDS in green berry stages successively, and finally, maximum ginsenosides accumulation occurred in the roots of American ginseng. Conclusion: We, therefore, hypothesized that cold-induced DNA methylation changes might regulate relative gene expression involving both plant development and plant secondary metabolites in such cold-dependent perennial plant species.http://www.sciencedirect.com/science/article/pii/S1226845319300272American ginsengCold temperatureGinsenosidesDNA methylationGene expression |
| spellingShingle | Mengzhen Hao Yuhang Zhou Jinhui Zhou Min Zhang Kangjiao Yan Sheng Jiang Wenshui Wang Xiaoping Peng San Zhou Cold-induced ginsenosides accumulation is associated with the alteration in DNA methylation and relative gene expression in perennial American ginseng (Panax quinquefolius L.) along with its plant growth and development process Journal of Ginseng Research American ginseng Cold temperature Ginsenosides DNA methylation Gene expression |
| title | Cold-induced ginsenosides accumulation is associated with the alteration in DNA methylation and relative gene expression in perennial American ginseng (Panax quinquefolius L.) along with its plant growth and development process |
| title_full | Cold-induced ginsenosides accumulation is associated with the alteration in DNA methylation and relative gene expression in perennial American ginseng (Panax quinquefolius L.) along with its plant growth and development process |
| title_fullStr | Cold-induced ginsenosides accumulation is associated with the alteration in DNA methylation and relative gene expression in perennial American ginseng (Panax quinquefolius L.) along with its plant growth and development process |
| title_full_unstemmed | Cold-induced ginsenosides accumulation is associated with the alteration in DNA methylation and relative gene expression in perennial American ginseng (Panax quinquefolius L.) along with its plant growth and development process |
| title_short | Cold-induced ginsenosides accumulation is associated with the alteration in DNA methylation and relative gene expression in perennial American ginseng (Panax quinquefolius L.) along with its plant growth and development process |
| title_sort | cold induced ginsenosides accumulation is associated with the alteration in dna methylation and relative gene expression in perennial american ginseng panax quinquefolius l along with its plant growth and development process |
| topic | American ginseng Cold temperature Ginsenosides DNA methylation Gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S1226845319300272 |
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