Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery tone
Suxiao Jiuxin Pill (SJP) is a well-known traditional Chinese medicine drug used to manage heart diseases. This study aimed at determining the pharmacological effects of SJP in acute myocardial infarction (AMI), and the molecular pathways its active compounds target to induce coronary artery vasorela...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1104243/full |
| _version_ | 1797830071448240128 |
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| author | Sa Li Jiaguo Zhan Yucheng Wang Patrick Kwabena Oduro Felix Boahen Owusu Jiale Zhang Ling Leng Ling Leng Ruiqiao Li Ruiqiao Li Shujie Wei Jun He Jun He Qilong Wang Qilong Wang Qilong Wang Qilong Wang |
| author_facet | Sa Li Jiaguo Zhan Yucheng Wang Patrick Kwabena Oduro Felix Boahen Owusu Jiale Zhang Ling Leng Ling Leng Ruiqiao Li Ruiqiao Li Shujie Wei Jun He Jun He Qilong Wang Qilong Wang Qilong Wang Qilong Wang |
| author_sort | Sa Li |
| collection | DOAJ |
| description | Suxiao Jiuxin Pill (SJP) is a well-known traditional Chinese medicine drug used to manage heart diseases. This study aimed at determining the pharmacological effects of SJP in acute myocardial infarction (AMI), and the molecular pathways its active compounds target to induce coronary artery vasorelaxation. Using the AMI rat model, SJP improved cardiac function and elevated ST segment. LC-MS and GC-MS detected twenty-eight non-volatile compounds and eleven volatile compounds in sera from SJP-treated rats. Network pharmacology analysis revealed eNOS and PTGS2 as the key drug targets. Indeed, SJP induced coronary artery relaxation via activation of the eNOS-NO pathway. Several of SJP’s main compounds, like senkyunolide A, scopoletin, and borneol, caused concentration-dependent coronary artery relaxation. Senkyunolide A and scopoletin increased eNOS and Akt phosphorylation in human umbilical vein endothelial cells (HUVECs). Molecular docking and surface plasmon resonance (SPR) revealed an interaction between senkynolide A/scopoletin and Akt. Vasodilation caused by senkyunolide A and scopoletin was inhibited by uprosertib (Akt inhibitor) and eNOS/sGC/PKG axis inhibitors. This suggests that senkyunolide A and scopoletin relax coronary arteries through the Akt-eNOS-NO pathway. In addition, borneol induced endothelium-independent vasorelaxation of the coronary artery. The Kv channel inhibitor 4-AP, KCa2+ inhibitor TEA, and Kir inhibitor BaCl2 significantly inhibited the vasorelaxant effect of borneol in the coronary artery. In conclusion, the results show that Suxiao Jiuxin Pill protects the heart against acute myocardial infarction. |
| first_indexed | 2024-04-09T13:30:16Z |
| format | Article |
| id | doaj.art-ff899674a08b4c1c94964d0acdedbd07 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-09T13:30:16Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-ff899674a08b4c1c94964d0acdedbd072023-05-10T04:54:39ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-05-011410.3389/fphar.2023.11042431104243Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery toneSa Li0Jiaguo Zhan1Yucheng Wang2Patrick Kwabena Oduro3Felix Boahen Owusu4Jiale Zhang5Ling Leng6Ling Leng7Ruiqiao Li8Ruiqiao Li9Shujie Wei10Jun He11Jun He12Qilong Wang13Qilong Wang14Qilong Wang15Qilong Wang16Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaState Key Laboratory of Component-Based Chinese Medicine, Ministry of Education, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaState Key Laboratory of Component-Based Chinese Medicine, Ministry of Education, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaState Key Laboratory of Component-Based Chinese Medicine, Ministry of Education, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaState Key Laboratory of Component-Based Chinese Medicine, Ministry of Education, Tianjin, ChinaHaihe Laboratory of Modern Chinese Medicine, Tianjin, ChinaEndocrinology Department, Fourth Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaSuxiao Jiuxin Pill (SJP) is a well-known traditional Chinese medicine drug used to manage heart diseases. This study aimed at determining the pharmacological effects of SJP in acute myocardial infarction (AMI), and the molecular pathways its active compounds target to induce coronary artery vasorelaxation. Using the AMI rat model, SJP improved cardiac function and elevated ST segment. LC-MS and GC-MS detected twenty-eight non-volatile compounds and eleven volatile compounds in sera from SJP-treated rats. Network pharmacology analysis revealed eNOS and PTGS2 as the key drug targets. Indeed, SJP induced coronary artery relaxation via activation of the eNOS-NO pathway. Several of SJP’s main compounds, like senkyunolide A, scopoletin, and borneol, caused concentration-dependent coronary artery relaxation. Senkyunolide A and scopoletin increased eNOS and Akt phosphorylation in human umbilical vein endothelial cells (HUVECs). Molecular docking and surface plasmon resonance (SPR) revealed an interaction between senkynolide A/scopoletin and Akt. Vasodilation caused by senkyunolide A and scopoletin was inhibited by uprosertib (Akt inhibitor) and eNOS/sGC/PKG axis inhibitors. This suggests that senkyunolide A and scopoletin relax coronary arteries through the Akt-eNOS-NO pathway. In addition, borneol induced endothelium-independent vasorelaxation of the coronary artery. The Kv channel inhibitor 4-AP, KCa2+ inhibitor TEA, and Kir inhibitor BaCl2 significantly inhibited the vasorelaxant effect of borneol in the coronary artery. In conclusion, the results show that Suxiao Jiuxin Pill protects the heart against acute myocardial infarction.https://www.frontiersin.org/articles/10.3389/fphar.2023.1104243/fullSuxiao Jiuxin Pillsenkyunolide ascopoletinborneolvasorelaxationacute myocardial infarction |
| spellingShingle | Sa Li Jiaguo Zhan Yucheng Wang Patrick Kwabena Oduro Felix Boahen Owusu Jiale Zhang Ling Leng Ling Leng Ruiqiao Li Ruiqiao Li Shujie Wei Jun He Jun He Qilong Wang Qilong Wang Qilong Wang Qilong Wang Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery tone Frontiers in Pharmacology Suxiao Jiuxin Pill senkyunolide a scopoletin borneol vasorelaxation acute myocardial infarction |
| title | Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery tone |
| title_full | Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery tone |
| title_fullStr | Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery tone |
| title_full_unstemmed | Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery tone |
| title_short | Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery tone |
| title_sort | suxiao jiuxin pill attenuates acute myocardial ischemia via regulation of coronary artery tone |
| topic | Suxiao Jiuxin Pill senkyunolide a scopoletin borneol vasorelaxation acute myocardial infarction |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1104243/full |
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