A tandem-repeat dimeric RBD protein-based covid-19 vaccine zf2001 protects mice and nonhuman primates
Safe, efficacious, and deployable vaccines are urgently needed to control COVID-19 in the large-scale vaccination campaigns. We report here the preclinical studies of an approved protein subunit vaccine against COVID-19, ZF2001, which contains tandem-repeat dimeric receptor-binding domain (RBD) prot...
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2022.2056524 |
| _version_ | 1819060359992967168 |
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| author | Yaling An Shihua Li Xiyue Jin Jian-bao Han Kun Xu Senyu Xu Yuxuan Han Chuanyu Liu Tianyi Zheng Mei Liu Mi Yang Tian-Zhang Song Baoying Huang Li Zhao Wen Wang Ruhan A Yingjie Cheng Changwei Wu Enqi Huang Shilong Yang Gary Wong Yuhai Bi Changwen Ke Wenjie Tan Jinghua Yan Yong-Tang Zheng Lianpan Dai George F. Gao |
| author_facet | Yaling An Shihua Li Xiyue Jin Jian-bao Han Kun Xu Senyu Xu Yuxuan Han Chuanyu Liu Tianyi Zheng Mei Liu Mi Yang Tian-Zhang Song Baoying Huang Li Zhao Wen Wang Ruhan A Yingjie Cheng Changwei Wu Enqi Huang Shilong Yang Gary Wong Yuhai Bi Changwen Ke Wenjie Tan Jinghua Yan Yong-Tang Zheng Lianpan Dai George F. Gao |
| author_sort | Yaling An |
| collection | DOAJ |
| description | Safe, efficacious, and deployable vaccines are urgently needed to control COVID-19 in the large-scale vaccination campaigns. We report here the preclinical studies of an approved protein subunit vaccine against COVID-19, ZF2001, which contains tandem-repeat dimeric receptor-binding domain (RBD) protein with alum-based adjuvant. We assessed vaccine immunogenicity and efficacy in both mice and non-human primates (NHPs). ZF2001 induced high levels of RBD-binding and SARS-CoV-2 neutralizing antibody in both mice and non-human primates, and elicited balanced TH1/TH2 cellular responses in NHPs. Two doses of ZF2001 protected Ad-hACE2-transduced mice against SARS-CoV-2 infection, as detected by reduced viral RNA and relieved lung injuries. In NHPs, vaccination of either 25 μg or 50 μg ZF2001 prevented infection with SARS-CoV-2 in lung, trachea, and bronchi, with milder lung lesions. No evidence of disease enhancement was observed in both animal models. ZF2001 has been approved for emergency use in China, Uzbekistan, Indonesia, and Columbia. The high safety, immunogenicity, and protection efficacy in both mice and NHPs found in this preclinical study was consistent with the results in human clinical trials. |
| first_indexed | 2024-12-21T14:25:45Z |
| format | Article |
| id | doaj.art-ff8a374f442344f5abd669d7a990dd88 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-12-21T14:25:45Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-ff8a374f442344f5abd669d7a990dd882022-12-21T19:00:40ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512022-12-011111058107110.1080/22221751.2022.2056524A tandem-repeat dimeric RBD protein-based covid-19 vaccine zf2001 protects mice and nonhuman primatesYaling An0Shihua Li1Xiyue Jin2Jian-bao Han3Kun Xu4Senyu Xu5Yuxuan Han6Chuanyu Liu7Tianyi Zheng8Mei Liu9Mi Yang10Tian-Zhang Song11Baoying Huang12Li Zhao13Wen Wang14Ruhan A15Yingjie Cheng16Changwei Wu17Enqi Huang18Shilong Yang19Gary Wong20Yuhai Bi21Changwen Ke22Wenjie Tan23Jinghua Yan24Yong-Tang Zheng25Lianpan Dai26George F. Gao27Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 101408, People’s Republic of ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of ChinaSchool of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, People’s Republic of ChinaKunming National High-Level Biosafety Research Center for Non-human Primates, Center for Biosafety Mega-Science, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650107, People’s Republic of ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, School of Tropical Medicine and Laboratory Medicine, The First Affiliated Hospital, Hainan Medical University, Hainan, 571199, People’s Republic of ChinaResearch Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of ChinaSavaid Medical School, University of Chinese Academy of Sciences, Beijing, 101408, People’s Republic of ChinaLaboratory of Animal Infectious Diseases, College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning, 530000, People’s Republic of ChinaResearch Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of ChinaCAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of ChinaCAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of ChinaKey Laboratory of Animal Models and Human Disease Mechanism of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academic of Sciences, Kunming, 650223, People’s Republic of ChinaNHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People’s Republic of ChinaNHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People’s Republic of ChinaNHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People’s Republic of ChinaNHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People’s Republic of ChinaAnhui Zhifei Longcom Biopharmaceutical Co. Ltd, Anhui, 230088, People’s Republic of ChinaAnhui Zhifei Longcom Biopharmaceutical Co. Ltd, Anhui, 230088, People’s Republic of ChinaAnhui Zhifei Longcom Biopharmaceutical Co. Ltd, Anhui, 230088, People’s Republic of ChinaAnhui Zhifei Longcom Biopharmaceutical Co. Ltd, Anhui, 230088, People’s Republic of ChinaCAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, People’s Republic of ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of ChinaGuangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, People’s Republic of ChinaNHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People’s Republic of ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of ChinaKunming National High-Level Biosafety Research Center for Non-human Primates, Center for Biosafety Mega-Science, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650107, People’s Republic of ChinaSavaid Medical School, University of Chinese Academy of Sciences, Beijing, 101408, People’s Republic of ChinaSavaid Medical School, University of Chinese Academy of Sciences, Beijing, 101408, People’s Republic of ChinaSafe, efficacious, and deployable vaccines are urgently needed to control COVID-19 in the large-scale vaccination campaigns. We report here the preclinical studies of an approved protein subunit vaccine against COVID-19, ZF2001, which contains tandem-repeat dimeric receptor-binding domain (RBD) protein with alum-based adjuvant. We assessed vaccine immunogenicity and efficacy in both mice and non-human primates (NHPs). ZF2001 induced high levels of RBD-binding and SARS-CoV-2 neutralizing antibody in both mice and non-human primates, and elicited balanced TH1/TH2 cellular responses in NHPs. Two doses of ZF2001 protected Ad-hACE2-transduced mice against SARS-CoV-2 infection, as detected by reduced viral RNA and relieved lung injuries. In NHPs, vaccination of either 25 μg or 50 μg ZF2001 prevented infection with SARS-CoV-2 in lung, trachea, and bronchi, with milder lung lesions. No evidence of disease enhancement was observed in both animal models. ZF2001 has been approved for emergency use in China, Uzbekistan, Indonesia, and Columbia. The high safety, immunogenicity, and protection efficacy in both mice and NHPs found in this preclinical study was consistent with the results in human clinical trials.https://www.tandfonline.com/doi/10.1080/22221751.2022.2056524Covid-19subunit protein vaccinezf2001antibodyimmune response |
| spellingShingle | Yaling An Shihua Li Xiyue Jin Jian-bao Han Kun Xu Senyu Xu Yuxuan Han Chuanyu Liu Tianyi Zheng Mei Liu Mi Yang Tian-Zhang Song Baoying Huang Li Zhao Wen Wang Ruhan A Yingjie Cheng Changwei Wu Enqi Huang Shilong Yang Gary Wong Yuhai Bi Changwen Ke Wenjie Tan Jinghua Yan Yong-Tang Zheng Lianpan Dai George F. Gao A tandem-repeat dimeric RBD protein-based covid-19 vaccine zf2001 protects mice and nonhuman primates Emerging Microbes and Infections Covid-19 subunit protein vaccine zf2001 antibody immune response |
| title | A tandem-repeat dimeric RBD protein-based covid-19 vaccine zf2001 protects mice and nonhuman primates |
| title_full | A tandem-repeat dimeric RBD protein-based covid-19 vaccine zf2001 protects mice and nonhuman primates |
| title_fullStr | A tandem-repeat dimeric RBD protein-based covid-19 vaccine zf2001 protects mice and nonhuman primates |
| title_full_unstemmed | A tandem-repeat dimeric RBD protein-based covid-19 vaccine zf2001 protects mice and nonhuman primates |
| title_short | A tandem-repeat dimeric RBD protein-based covid-19 vaccine zf2001 protects mice and nonhuman primates |
| title_sort | tandem repeat dimeric rbd protein based covid 19 vaccine zf2001 protects mice and nonhuman primates |
| topic | Covid-19 subunit protein vaccine zf2001 antibody immune response |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2022.2056524 |
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