Optimization of solvent-free microwave-assisted extraction of antioxidant compounds from Lagenaria siceraria fruit by response surface methodology
Lagenaria siceraria (bottle gourd) fruit is highly celebrated for its nutritional as well as therapeutic properties. The present study was undertaken with the aim of exploring an efficient green solvent-free microwave-assisted extraction method for bioactives from this fruit. Optimization was done a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2022-08-01
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| Series: | Folia Horticulturae |
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| Online Access: | https://doi.org/10.2478/fhort-2022-0013 |
| _version_ | 1811159408606969856 |
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| author | Iftikhar Sahar Tariq Qamar Muhammad Aydar Alev Yüksel Ahmed Dildar |
| author_facet | Iftikhar Sahar Tariq Qamar Muhammad Aydar Alev Yüksel Ahmed Dildar |
| author_sort | Iftikhar Sahar |
| collection | DOAJ |
| description | Lagenaria siceraria (bottle gourd) fruit is highly celebrated for its nutritional as well as therapeutic properties. The present study was undertaken with the aim of exploring an efficient green solvent-free microwave-assisted extraction method for bioactives from this fruit. Optimization was done according to response surface methodology (RSM), where microwave power (W) and time (s) were independent factors, and percent extraction yield, total phenolic content (TPC), total flavonoid content (TFC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant potential (FRAP) and iron chelating activity (ICA) were the responses. TPC, TFC, DPPH radical scavenging activity, FRAP and ICA were highest at 480 W and 60 s. The TPC was 288.9 mg GAE · g−1 DW (milligram gallic acid equivalent per gram dry weight), TFC was 214.1 mg RE · g−1 (rutin equivalent per gram DW), anti-radical activity was 32.96%, FRAP was 289.7 mg AAE · g−1 (mg ascorbic acid equivalents per gram) and ICA was 19.52%. The results of the study thus demonstrate that the solvent-free microwave-assisted extraction method, which utilised an optimum power of 480 W and a time of 60 s, is an effective and green method for extraction of antioxidant compounds from bottle gourd fruit. |
| first_indexed | 2024-04-10T05:40:34Z |
| format | Article |
| id | doaj.art-ff8baf0b1db343859318a170da95b882 |
| institution | Directory Open Access Journal |
| issn | 2083-5965 |
| language | English |
| last_indexed | 2024-04-10T05:40:34Z |
| publishDate | 2022-08-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Folia Horticulturae |
| spelling | doaj.art-ff8baf0b1db343859318a170da95b8822023-03-06T10:29:55ZengSciendoFolia Horticulturae2083-59652022-08-0134216317110.2478/fhort-2022-0013Optimization of solvent-free microwave-assisted extraction of antioxidant compounds from Lagenaria siceraria fruit by response surface methodologyIftikhar Sahar0Tariq Qamar Muhammad1Aydar Alev Yüksel2Ahmed Dildar3Department of Chemistry, Forman Christian College (A Chartered University), Lahore, PakistanDepartment of Chemistry, Forman Christian College (A Chartered University), Lahore, PakistanDepartment of Food Engineering, Manisa Celal Bayar University, Manisa, TurkeyDepartment of Chemistry, Forman Christian College (A Chartered University), Lahore, PakistanLagenaria siceraria (bottle gourd) fruit is highly celebrated for its nutritional as well as therapeutic properties. The present study was undertaken with the aim of exploring an efficient green solvent-free microwave-assisted extraction method for bioactives from this fruit. Optimization was done according to response surface methodology (RSM), where microwave power (W) and time (s) were independent factors, and percent extraction yield, total phenolic content (TPC), total flavonoid content (TFC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant potential (FRAP) and iron chelating activity (ICA) were the responses. TPC, TFC, DPPH radical scavenging activity, FRAP and ICA were highest at 480 W and 60 s. The TPC was 288.9 mg GAE · g−1 DW (milligram gallic acid equivalent per gram dry weight), TFC was 214.1 mg RE · g−1 (rutin equivalent per gram DW), anti-radical activity was 32.96%, FRAP was 289.7 mg AAE · g−1 (mg ascorbic acid equivalents per gram) and ICA was 19.52%. The results of the study thus demonstrate that the solvent-free microwave-assisted extraction method, which utilised an optimum power of 480 W and a time of 60 s, is an effective and green method for extraction of antioxidant compounds from bottle gourd fruit.https://doi.org/10.2478/fhort-2022-0013antioxidantbottle gourdgreen extractioniron chelationphenolics |
| spellingShingle | Iftikhar Sahar Tariq Qamar Muhammad Aydar Alev Yüksel Ahmed Dildar Optimization of solvent-free microwave-assisted extraction of antioxidant compounds from Lagenaria siceraria fruit by response surface methodology Folia Horticulturae antioxidant bottle gourd green extraction iron chelation phenolics |
| title | Optimization of solvent-free microwave-assisted extraction of antioxidant compounds from Lagenaria siceraria fruit by response surface methodology |
| title_full | Optimization of solvent-free microwave-assisted extraction of antioxidant compounds from Lagenaria siceraria fruit by response surface methodology |
| title_fullStr | Optimization of solvent-free microwave-assisted extraction of antioxidant compounds from Lagenaria siceraria fruit by response surface methodology |
| title_full_unstemmed | Optimization of solvent-free microwave-assisted extraction of antioxidant compounds from Lagenaria siceraria fruit by response surface methodology |
| title_short | Optimization of solvent-free microwave-assisted extraction of antioxidant compounds from Lagenaria siceraria fruit by response surface methodology |
| title_sort | optimization of solvent free microwave assisted extraction of antioxidant compounds from lagenaria siceraria fruit by response surface methodology |
| topic | antioxidant bottle gourd green extraction iron chelation phenolics |
| url | https://doi.org/10.2478/fhort-2022-0013 |
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