Abstract OR-9: Cryo-Electron Microscopy Studies of Flaviviruses
Background: Flaviviruses cause human disease such as dengue fever, West Nile fever, and Zika fever with high degree of severity. There are almost no licensed vaccines and treatment except symptomatic for these diseases. Our group studies the virus structure and interactions with ligands such as rece...
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| Format: | Article |
| Language: | English |
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International Medical Research and Development Corporation
2019-06-01
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| Series: | International Journal of Biomedicine |
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| Online Access: | http://ijbm.org/articles/IJBM_2019_9_S1_OR9.pdf |
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| author | Victor A. Kostyuchenko Guntur Fibriansah Shuijun Zhang Thiam-Seng Ng Shee-Mei Lok |
| author_facet | Victor A. Kostyuchenko Guntur Fibriansah Shuijun Zhang Thiam-Seng Ng Shee-Mei Lok |
| author_sort | Victor A. Kostyuchenko |
| collection | DOAJ |
| description | Background: Flaviviruses cause human disease such as dengue fever, West Nile fever, and Zika fever with high degree of severity. There are almost no licensed vaccines and treatment except symptomatic for these diseases. Our group studies the virus structure and interactions with ligands such as receptors and antibodies in order to understand the virus’ life cycle with the aim to find possible preventive or curative drugs.
Methods: For structural biology work, we employ mostly cryo-EM such as single particle analysis and cryo-electron tomography to study complexes of the virus with antibody fragments.
Results: We obtained subnanometer and near-atomic resolution cryo-EM structures for immature and mature forms of dengue virus serotype 1, dengue virus serotype 4, and zika virus. We described a possible maturation pathway based on the higher resolution structures. We also described a temperature-dependent structural change that occurs in some strains of the flaviviruses, important for vaccine development. In addition, we described a cryo-EM structure of a complex of a dengue virus and antibody fragment that demonstrates that in some cases cryo-EM is superior to X-ray crystallography as providing a fuller picture of the virus and antibody interaction.
Conclusion: Modern cryo-EM allows us a better and more detailed look at the viruses, their life cycle and help the development of a better vaccines and therapeutics. |
| first_indexed | 2024-04-13T00:26:18Z |
| format | Article |
| id | doaj.art-ff8f695709a24dfebaa1ccb77eeefac1 |
| institution | Directory Open Access Journal |
| issn | 2158-0510 2158-0529 |
| language | English |
| last_indexed | 2024-04-13T00:26:18Z |
| publishDate | 2019-06-01 |
| publisher | International Medical Research and Development Corporation |
| record_format | Article |
| series | International Journal of Biomedicine |
| spelling | doaj.art-ff8f695709a24dfebaa1ccb77eeefac12022-12-22T03:10:37ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292019-06-019Suppl_1S9S910.21103/IJBM.9.Suppl_1.OR9Abstract OR-9: Cryo-Electron Microscopy Studies of FlavivirusesVictor A. Kostyuchenko0Guntur Fibriansah1Shuijun Zhang2Thiam-Seng Ng3Shee-Mei Lok4Virus structure and function group, Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore Virus structure and function group, Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore Virus structure and function group, Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore Virus structure and function group, Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore Virus structure and function group, Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore Background: Flaviviruses cause human disease such as dengue fever, West Nile fever, and Zika fever with high degree of severity. There are almost no licensed vaccines and treatment except symptomatic for these diseases. Our group studies the virus structure and interactions with ligands such as receptors and antibodies in order to understand the virus’ life cycle with the aim to find possible preventive or curative drugs. Methods: For structural biology work, we employ mostly cryo-EM such as single particle analysis and cryo-electron tomography to study complexes of the virus with antibody fragments. Results: We obtained subnanometer and near-atomic resolution cryo-EM structures for immature and mature forms of dengue virus serotype 1, dengue virus serotype 4, and zika virus. We described a possible maturation pathway based on the higher resolution structures. We also described a temperature-dependent structural change that occurs in some strains of the flaviviruses, important for vaccine development. In addition, we described a cryo-EM structure of a complex of a dengue virus and antibody fragment that demonstrates that in some cases cryo-EM is superior to X-ray crystallography as providing a fuller picture of the virus and antibody interaction. Conclusion: Modern cryo-EM allows us a better and more detailed look at the viruses, their life cycle and help the development of a better vaccines and therapeutics.http://ijbm.org/articles/IJBM_2019_9_S1_OR9.pdfDengue virusZika virusFlavivirusCryo-EM |
| spellingShingle | Victor A. Kostyuchenko Guntur Fibriansah Shuijun Zhang Thiam-Seng Ng Shee-Mei Lok Abstract OR-9: Cryo-Electron Microscopy Studies of Flaviviruses International Journal of Biomedicine Dengue virus Zika virus Flavivirus Cryo-EM |
| title | Abstract OR-9: Cryo-Electron Microscopy Studies of Flaviviruses |
| title_full | Abstract OR-9: Cryo-Electron Microscopy Studies of Flaviviruses |
| title_fullStr | Abstract OR-9: Cryo-Electron Microscopy Studies of Flaviviruses |
| title_full_unstemmed | Abstract OR-9: Cryo-Electron Microscopy Studies of Flaviviruses |
| title_short | Abstract OR-9: Cryo-Electron Microscopy Studies of Flaviviruses |
| title_sort | abstract or 9 cryo electron microscopy studies of flaviviruses |
| topic | Dengue virus Zika virus Flavivirus Cryo-EM |
| url | http://ijbm.org/articles/IJBM_2019_9_S1_OR9.pdf |
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