A Pilot Study on the Urine Proteome of Cats Fed a High-Protein Complete Diet, Supplemented with or without Arginine, Ornithine or Zeolite
Proteome analyses can be used to detect biomarkers for the healthy and diseased organism. However, data in cats are scarce, and no information is available on the potential impact of nutritional interventions on the feline urine proteome. In the present study, a label-free shotgun proteomics approac...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/12/654 |
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| author | Nadine Paßlack Katharina Nöbauer Karin Hummel Ebrahim Razzazi-Fazeli Vitaly Belik Jürgen Zentek |
| author_facet | Nadine Paßlack Katharina Nöbauer Karin Hummel Ebrahim Razzazi-Fazeli Vitaly Belik Jürgen Zentek |
| author_sort | Nadine Paßlack |
| collection | DOAJ |
| description | Proteome analyses can be used to detect biomarkers for the healthy and diseased organism. However, data in cats are scarce, and no information is available on the potential impact of nutritional interventions on the feline urine proteome. In the present study, a label-free shotgun proteomics approach was performed to investigate the urinary proteins of four healthy adult cats. Each animal received a high-protein complete diet without (w/o) or with supplements that could affect the protein metabolism: arginine (+100% compared to the arginine concentration in the w/o diet), ornithine (+200% compared to the arginine concentration in the w/o diet) or zeolite (0.375 g/kg body weight/day). Our results demonstrate a huge number of proteins in the urine of cats (516 ± 49, 512 ± 39, 399 ± 149 and 455 ± 134 in the w/o, arginine, ornithine and zeolite group, respectively), which are associated with several biological processes. In addition, up- and downregulated urinary proteins could be detected in the dietary supplementation periods. Overall, the present pilot study provides basic data on the urine proteome of healthy adult cats. With increasing information, the numerousness of urinary proteins implies the potential to identify biomarkers and metabolic pathways in the feline organism. |
| first_indexed | 2024-03-09T15:46:19Z |
| format | Article |
| id | doaj.art-ff91baf57f754c6bad897a7a22fed31b |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T15:46:19Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-ff91baf57f754c6bad897a7a22fed31b2023-11-24T18:34:48ZengMDPI AGVeterinary Sciences2306-73812022-11-0191265410.3390/vetsci9120654A Pilot Study on the Urine Proteome of Cats Fed a High-Protein Complete Diet, Supplemented with or without Arginine, Ornithine or ZeoliteNadine Paßlack0Katharina Nöbauer1Karin Hummel2Ebrahim Razzazi-Fazeli3Vitaly Belik4Jürgen Zentek5Institute of Animal Nutrition, Freie Universität Berlin, 14195 Berlin, GermanyVetCore Facility for Research, University of Veterinary Medicine, 1210 Vienna, AustriaVetCore Facility for Research, University of Veterinary Medicine, 1210 Vienna, AustriaVetCore Facility for Research, University of Veterinary Medicine, 1210 Vienna, AustriaInstitute for Veterinary Epidemiology and Biostatistics, Freie Universität Berlin, 14163 Berlin, GermanyInstitute of Animal Nutrition, Freie Universität Berlin, 14195 Berlin, GermanyProteome analyses can be used to detect biomarkers for the healthy and diseased organism. However, data in cats are scarce, and no information is available on the potential impact of nutritional interventions on the feline urine proteome. In the present study, a label-free shotgun proteomics approach was performed to investigate the urinary proteins of four healthy adult cats. Each animal received a high-protein complete diet without (w/o) or with supplements that could affect the protein metabolism: arginine (+100% compared to the arginine concentration in the w/o diet), ornithine (+200% compared to the arginine concentration in the w/o diet) or zeolite (0.375 g/kg body weight/day). Our results demonstrate a huge number of proteins in the urine of cats (516 ± 49, 512 ± 39, 399 ± 149 and 455 ± 134 in the w/o, arginine, ornithine and zeolite group, respectively), which are associated with several biological processes. In addition, up- and downregulated urinary proteins could be detected in the dietary supplementation periods. Overall, the present pilot study provides basic data on the urine proteome of healthy adult cats. With increasing information, the numerousness of urinary proteins implies the potential to identify biomarkers and metabolic pathways in the feline organism.https://www.mdpi.com/2306-7381/9/12/654urinefelineproteomeliquid chromatography coupled to mass spectrometrynutrition |
| spellingShingle | Nadine Paßlack Katharina Nöbauer Karin Hummel Ebrahim Razzazi-Fazeli Vitaly Belik Jürgen Zentek A Pilot Study on the Urine Proteome of Cats Fed a High-Protein Complete Diet, Supplemented with or without Arginine, Ornithine or Zeolite Veterinary Sciences urine feline proteome liquid chromatography coupled to mass spectrometry nutrition |
| title | A Pilot Study on the Urine Proteome of Cats Fed a High-Protein Complete Diet, Supplemented with or without Arginine, Ornithine or Zeolite |
| title_full | A Pilot Study on the Urine Proteome of Cats Fed a High-Protein Complete Diet, Supplemented with or without Arginine, Ornithine or Zeolite |
| title_fullStr | A Pilot Study on the Urine Proteome of Cats Fed a High-Protein Complete Diet, Supplemented with or without Arginine, Ornithine or Zeolite |
| title_full_unstemmed | A Pilot Study on the Urine Proteome of Cats Fed a High-Protein Complete Diet, Supplemented with or without Arginine, Ornithine or Zeolite |
| title_short | A Pilot Study on the Urine Proteome of Cats Fed a High-Protein Complete Diet, Supplemented with or without Arginine, Ornithine or Zeolite |
| title_sort | pilot study on the urine proteome of cats fed a high protein complete diet supplemented with or without arginine ornithine or zeolite |
| topic | urine feline proteome liquid chromatography coupled to mass spectrometry nutrition |
| url | https://www.mdpi.com/2306-7381/9/12/654 |
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