Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (<i>Pisum sativum</i> L.) Germplasms
Pea (<i>Pisum sativum</i> L.) is an important legume crop. However, the yield of pea is adversely affected by heat stress in China. In this study, heat-tolerant germplasms were screened and evaluated in the field under multi-conditions. The results showed that heat stress could significa...
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MDPI AG
2022-09-01
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| author | Dong Wang Tao Yang Rong Liu Nana Li Naveed Ahmad Guan Li Yishan Ji Chenyu Wang Mengwei Li Xin Yan Hanfeng Ding Xuxiao Zong |
| author_facet | Dong Wang Tao Yang Rong Liu Nana Li Naveed Ahmad Guan Li Yishan Ji Chenyu Wang Mengwei Li Xin Yan Hanfeng Ding Xuxiao Zong |
| author_sort | Dong Wang |
| collection | DOAJ |
| description | Pea (<i>Pisum sativum</i> L.) is an important legume crop. However, the yield of pea is adversely affected by heat stress in China. In this study, heat-tolerant germplasms were screened and evaluated in the field under multi-conditions. The results showed that heat stress could significantly affect pea yield. On the basis of grain weight per plant, 257 heat-tolerant and 175 heat-sensitive accessions were obtained from the first year’s screening, and 26 extremely heat-tolerant and 19 extremely heat-sensitive accessions were finally obtained in this study. Based on SNaPshot technology, two sets of SNP markers, including 46 neutral and 20 heat-tolerance-related markers, were used to evaluate the genetic diversity and population genetic structure of the 432 pea accessions obtained from the first year’s screening. Genetic diversity analysis showed that the average polymorphic information content was lower using heat-tolerance-related markers than neutral markers because of the selective pressure under heat stress. In addition, population genetic structure analysis showed that neutral markers divided the 432 pea accessions into two subpopulations associated with sowing date type and geographical origin, while the heat-tolerance-related markers divided these germplasms into two subpopulations associated with heat tolerance and sowing date type. Overall, we present a comprehensive resource of heat-tolerant and heat-sensitive pea accessions through heat-tolerance screenings in multi-conditions, which could help genetic improvements of pea in the future. |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-09T21:18:26Z |
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| series | Plants |
| spelling | doaj.art-ff9243f50e9e4844880a08f5583078c52023-11-23T21:28:04ZengMDPI AGPlants2223-77472022-09-011119247310.3390/plants11192473Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (<i>Pisum sativum</i> L.) GermplasmsDong Wang0Tao Yang1Rong Liu2Nana Li3Naveed Ahmad4Guan Li5Yishan Ji6Chenyu Wang7Mengwei Li8Xin Yan9Hanfeng Ding10Xuxiao Zong11Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaPea (<i>Pisum sativum</i> L.) is an important legume crop. However, the yield of pea is adversely affected by heat stress in China. In this study, heat-tolerant germplasms were screened and evaluated in the field under multi-conditions. The results showed that heat stress could significantly affect pea yield. On the basis of grain weight per plant, 257 heat-tolerant and 175 heat-sensitive accessions were obtained from the first year’s screening, and 26 extremely heat-tolerant and 19 extremely heat-sensitive accessions were finally obtained in this study. Based on SNaPshot technology, two sets of SNP markers, including 46 neutral and 20 heat-tolerance-related markers, were used to evaluate the genetic diversity and population genetic structure of the 432 pea accessions obtained from the first year’s screening. Genetic diversity analysis showed that the average polymorphic information content was lower using heat-tolerance-related markers than neutral markers because of the selective pressure under heat stress. In addition, population genetic structure analysis showed that neutral markers divided the 432 pea accessions into two subpopulations associated with sowing date type and geographical origin, while the heat-tolerance-related markers divided these germplasms into two subpopulations associated with heat tolerance and sowing date type. Overall, we present a comprehensive resource of heat-tolerant and heat-sensitive pea accessions through heat-tolerance screenings in multi-conditions, which could help genetic improvements of pea in the future.https://www.mdpi.com/2223-7747/11/19/2473pea (<i>Pisum sativum</i> L.)heat-tolerance screeningSNaPshotgenetic diversitypopulation genetic structure |
| spellingShingle | Dong Wang Tao Yang Rong Liu Nana Li Naveed Ahmad Guan Li Yishan Ji Chenyu Wang Mengwei Li Xin Yan Hanfeng Ding Xuxiao Zong Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (<i>Pisum sativum</i> L.) Germplasms Plants pea (<i>Pisum sativum</i> L.) heat-tolerance screening SNaPshot genetic diversity population genetic structure |
| title | Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (<i>Pisum sativum</i> L.) Germplasms |
| title_full | Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (<i>Pisum sativum</i> L.) Germplasms |
| title_fullStr | Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (<i>Pisum sativum</i> L.) Germplasms |
| title_full_unstemmed | Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (<i>Pisum sativum</i> L.) Germplasms |
| title_short | Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (<i>Pisum sativum</i> L.) Germplasms |
| title_sort | large scale heat tolerance screening and genetic diversity of pea i pisum sativum i l germplasms |
| topic | pea (<i>Pisum sativum</i> L.) heat-tolerance screening SNaPshot genetic diversity population genetic structure |
| url | https://www.mdpi.com/2223-7747/11/19/2473 |
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