Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency Than Parental Lines by F1 Microspore Embryogenesis
Creating varieties with high nitrogen use efficiency (NUE) is crucial for sustainable agriculture development. In this study, a superior barley doubled haploid line (named DH45) with improved NUE was produced via F<sub>1</sub> microspore embryogenesis with three rounds of screening in di...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2223-7747/10/8/1588 |
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| author | Hongwei Xu Yingbo Li Runhong Gao Rugen Xu Guimei Guo Ruiju Lu Nigel G. Halford Zhiwei Chen Chenghong Liu |
| author_facet | Hongwei Xu Yingbo Li Runhong Gao Rugen Xu Guimei Guo Ruiju Lu Nigel G. Halford Zhiwei Chen Chenghong Liu |
| author_sort | Hongwei Xu |
| collection | DOAJ |
| description | Creating varieties with high nitrogen use efficiency (NUE) is crucial for sustainable agriculture development. In this study, a superior barley doubled haploid line (named DH45) with improved NUE was produced via F<sub>1</sub> microspore embryogenesis with three rounds of screening in different nitrogen levels by hydroponic and field experiments. The molecular mechanisms responsible for the NUE of DH45 surpassing that of its parents were investigated by RNA-seq analysis. A total of 1027 differentially expressed genes (DEGs) were identified that were up- or down-regulated in DH45 under low nitrogen conditions but showed no significant differences in the parents. GO analysis indicated that genes involved in nitrogen compound metabolic processes were significantly enriched in DH45 compared with the parents. KEGG analysis showed the MAPK signaling pathway plant to be highly enriched in DH45 relative to its parents, as well as genes involved in alanine, aspartate and glutamate metabolism, and arginine biosynthesis. In conclusion, our study revealed the potential to fix trait superiority in a line by combining crossing with F<sub>1</sub> microspore culture technologies in future crop breeding and also identified several candidate genes that are expressed in shoots and may enable barley to cope with low-nitrogen stress. |
| first_indexed | 2024-03-10T08:28:53Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T08:28:53Z |
| publishDate | 2021-08-01 |
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| series | Plants |
| spelling | doaj.art-ff9323f9f8cf47f69f3ff0bc5d6c3e6e2023-11-22T09:18:33ZengMDPI AGPlants2223-77472021-08-01108158810.3390/plants10081588Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency Than Parental Lines by F1 Microspore EmbryogenesisHongwei Xu0Yingbo Li1Runhong Gao2Rugen Xu3Guimei Guo4Ruiju Lu5Nigel G. Halford6Zhiwei Chen7Chenghong Liu8Shanghai Academy of Agricultural Sciences/Key Laboratory of Agricultural Genetics and Breeding, Biotech Research Institute, Shanghai 201106, ChinaShanghai Academy of Agricultural Sciences/Key Laboratory of Agricultural Genetics and Breeding, Biotech Research Institute, Shanghai 201106, ChinaShanghai Academy of Agricultural Sciences/Key Laboratory of Agricultural Genetics and Breeding, Biotech Research Institute, Shanghai 201106, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Barley Research Institution of Yangzhou University, Yangzhou University, Yangzhou 225009, ChinaShanghai Academy of Agricultural Sciences/Key Laboratory of Agricultural Genetics and Breeding, Biotech Research Institute, Shanghai 201106, ChinaShanghai Academy of Agricultural Sciences/Key Laboratory of Agricultural Genetics and Breeding, Biotech Research Institute, Shanghai 201106, ChinaDepartment of Plant Sciences, Rothamsted Research, Harpenden AL5 2JQ, UKShanghai Academy of Agricultural Sciences/Key Laboratory of Agricultural Genetics and Breeding, Biotech Research Institute, Shanghai 201106, ChinaShanghai Academy of Agricultural Sciences/Key Laboratory of Agricultural Genetics and Breeding, Biotech Research Institute, Shanghai 201106, ChinaCreating varieties with high nitrogen use efficiency (NUE) is crucial for sustainable agriculture development. In this study, a superior barley doubled haploid line (named DH45) with improved NUE was produced via F<sub>1</sub> microspore embryogenesis with three rounds of screening in different nitrogen levels by hydroponic and field experiments. The molecular mechanisms responsible for the NUE of DH45 surpassing that of its parents were investigated by RNA-seq analysis. A total of 1027 differentially expressed genes (DEGs) were identified that were up- or down-regulated in DH45 under low nitrogen conditions but showed no significant differences in the parents. GO analysis indicated that genes involved in nitrogen compound metabolic processes were significantly enriched in DH45 compared with the parents. KEGG analysis showed the MAPK signaling pathway plant to be highly enriched in DH45 relative to its parents, as well as genes involved in alanine, aspartate and glutamate metabolism, and arginine biosynthesis. In conclusion, our study revealed the potential to fix trait superiority in a line by combining crossing with F<sub>1</sub> microspore culture technologies in future crop breeding and also identified several candidate genes that are expressed in shoots and may enable barley to cope with low-nitrogen stress.https://www.mdpi.com/2223-7747/10/8/1588barleymicrosporedoubled haploid linenitrogen use efficiencyRNA-seq |
| spellingShingle | Hongwei Xu Yingbo Li Runhong Gao Rugen Xu Guimei Guo Ruiju Lu Nigel G. Halford Zhiwei Chen Chenghong Liu Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency Than Parental Lines by F1 Microspore Embryogenesis Plants barley microspore doubled haploid line nitrogen use efficiency RNA-seq |
| title | Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency Than Parental Lines by F1 Microspore Embryogenesis |
| title_full | Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency Than Parental Lines by F1 Microspore Embryogenesis |
| title_fullStr | Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency Than Parental Lines by F1 Microspore Embryogenesis |
| title_full_unstemmed | Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency Than Parental Lines by F1 Microspore Embryogenesis |
| title_short | Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency Than Parental Lines by F1 Microspore Embryogenesis |
| title_sort | rapid generation and analysis of a barley doubled haploid line with higher nitrogen use efficiency than parental lines by f1 microspore embryogenesis |
| topic | barley microspore doubled haploid line nitrogen use efficiency RNA-seq |
| url | https://www.mdpi.com/2223-7747/10/8/1588 |
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