A comparative analyses of lipid ratios representing desaturase enzyme activity between preterm and term infants within the first ten weeks of life
Abstract Background Desaturase enzymes play a key role in several pathways including biosynthesis of poly- and mono- unsaturated fatty acids (PUFAs, MUFA). In preterm infants, desaturase enzyme activity (DA) may be a rate-limiting step in maintaining PUFAs levels during this critical developmental w...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Lipids in Health and Disease |
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| Online Access: | https://doi.org/10.1186/s12944-023-01862-8 |
| _version_ | 1797556822962339840 |
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| author | Hanis Hidayu Kasim Laurentya Olga Stuart Snowden Eliza Cropp Albert Koulman Kathryn Beardsall |
| author_facet | Hanis Hidayu Kasim Laurentya Olga Stuart Snowden Eliza Cropp Albert Koulman Kathryn Beardsall |
| author_sort | Hanis Hidayu Kasim |
| collection | DOAJ |
| description | Abstract Background Desaturase enzymes play a key role in several pathways including biosynthesis of poly- and mono- unsaturated fatty acids (PUFAs, MUFA). In preterm infants, desaturase enzyme activity (DA) may be a rate-limiting step in maintaining PUFAs levels during this critical developmental window and impact on long term metabolic health. The study tested the hypothesis that DA is altered in preterm infants compared to term infants in early life and may be a marker of risk or contribute to later alterations in metabolic health. Methods Lipidomic analyses were conducted using blood samples from two established UK-based cohorts, involving very preterm (n = 105) and term (n = 259) infants. Blood samples were taken from term infants at birth, two and six weeks and from preterm infants when established on enteral feeds and at term corrected age. DA of the 2 groups of infants were estimated indirectly from product/precursor lipids ratios of phosphatidylcholine (PC) and triglycerides (TG) species and reported according to their postmenstrual and postnatal ages. Results There were changes in lipid ratios representing desaturase enzyme activity in preterm infants in the first weeks of life with higher delta 6 desaturases (D6D) triglyceride (TG) indices but significantly lower delta 9 desaturase (D9D) and D6D(PC) indices. In comparison to term infants, preterm have lower delta 5 desaturase (D5D) but higher D6D indices at all postnatal ages. Although point levels of desaturase indices were different, trajectories of changes in these indices over time were similar in preterm and term infants. Conclusions This study findings suggest the patterns of desaturase indices in preterm infants differ from that of term infants but their trajectories of change in the first 10 weeks of life were similar. These differences of DA if they persist in later life could contribute to the mechanism of diseases in preterm adulthood and warrant further investigations. |
| first_indexed | 2024-03-10T17:08:26Z |
| format | Article |
| id | doaj.art-ff9b2afee18a4819b3d18c5ead415a11 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-03-10T17:08:26Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-ff9b2afee18a4819b3d18c5ead415a112023-11-20T10:45:47ZengBMCLipids in Health and Disease1476-511X2023-08-012211810.1186/s12944-023-01862-8A comparative analyses of lipid ratios representing desaturase enzyme activity between preterm and term infants within the first ten weeks of lifeHanis Hidayu Kasim0Laurentya Olga1Stuart Snowden2Eliza Cropp3Albert Koulman4Kathryn Beardsall5Department of Paediatrics, University of CambridgeDepartment of Paediatrics, University of CambridgeWellcome-MRC Institute of Metabolic Science-Metabolic Research Laboratories, University of CambridgeDepartment of Paediatrics, University of CambridgeWellcome-MRC Institute of Metabolic Science-Metabolic Research Laboratories, University of CambridgeDepartment of Paediatrics, University of CambridgeAbstract Background Desaturase enzymes play a key role in several pathways including biosynthesis of poly- and mono- unsaturated fatty acids (PUFAs, MUFA). In preterm infants, desaturase enzyme activity (DA) may be a rate-limiting step in maintaining PUFAs levels during this critical developmental window and impact on long term metabolic health. The study tested the hypothesis that DA is altered in preterm infants compared to term infants in early life and may be a marker of risk or contribute to later alterations in metabolic health. Methods Lipidomic analyses were conducted using blood samples from two established UK-based cohorts, involving very preterm (n = 105) and term (n = 259) infants. Blood samples were taken from term infants at birth, two and six weeks and from preterm infants when established on enteral feeds and at term corrected age. DA of the 2 groups of infants were estimated indirectly from product/precursor lipids ratios of phosphatidylcholine (PC) and triglycerides (TG) species and reported according to their postmenstrual and postnatal ages. Results There were changes in lipid ratios representing desaturase enzyme activity in preterm infants in the first weeks of life with higher delta 6 desaturases (D6D) triglyceride (TG) indices but significantly lower delta 9 desaturase (D9D) and D6D(PC) indices. In comparison to term infants, preterm have lower delta 5 desaturase (D5D) but higher D6D indices at all postnatal ages. Although point levels of desaturase indices were different, trajectories of changes in these indices over time were similar in preterm and term infants. Conclusions This study findings suggest the patterns of desaturase indices in preterm infants differ from that of term infants but their trajectories of change in the first 10 weeks of life were similar. These differences of DA if they persist in later life could contribute to the mechanism of diseases in preterm adulthood and warrant further investigations.https://doi.org/10.1186/s12944-023-01862-8Desaturase enzymePreterm infantsLCPUFAsMetabolism |
| spellingShingle | Hanis Hidayu Kasim Laurentya Olga Stuart Snowden Eliza Cropp Albert Koulman Kathryn Beardsall A comparative analyses of lipid ratios representing desaturase enzyme activity between preterm and term infants within the first ten weeks of life Lipids in Health and Disease Desaturase enzyme Preterm infants LCPUFAs Metabolism |
| title | A comparative analyses of lipid ratios representing desaturase enzyme activity between preterm and term infants within the first ten weeks of life |
| title_full | A comparative analyses of lipid ratios representing desaturase enzyme activity between preterm and term infants within the first ten weeks of life |
| title_fullStr | A comparative analyses of lipid ratios representing desaturase enzyme activity between preterm and term infants within the first ten weeks of life |
| title_full_unstemmed | A comparative analyses of lipid ratios representing desaturase enzyme activity between preterm and term infants within the first ten weeks of life |
| title_short | A comparative analyses of lipid ratios representing desaturase enzyme activity between preterm and term infants within the first ten weeks of life |
| title_sort | comparative analyses of lipid ratios representing desaturase enzyme activity between preterm and term infants within the first ten weeks of life |
| topic | Desaturase enzyme Preterm infants LCPUFAs Metabolism |
| url | https://doi.org/10.1186/s12944-023-01862-8 |
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