Multi-parametric characterization of drug effects on cells [version 1; peer review: 2 approved]
We present here a novel multi-parametric approach for the characterization of multiple cellular features, using images acquired by high-throughput and high-definition light microscopy. We specifically used this approach for deep and unbiased analysis of the effects of a drug library on five cultured...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2020-10-01
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| Series: | F1000Research |
| Online Access: | https://f1000research.com/articles/9-1199/v1 |
| _version_ | 1818331961017499648 |
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| author | Yael Paran Yuvalal Liron Sarit Batsir Nicola Mabjeesh Benjamin Geiger Zvi Kam |
| author_facet | Yael Paran Yuvalal Liron Sarit Batsir Nicola Mabjeesh Benjamin Geiger Zvi Kam |
| author_sort | Yael Paran |
| collection | DOAJ |
| description | We present here a novel multi-parametric approach for the characterization of multiple cellular features, using images acquired by high-throughput and high-definition light microscopy. We specifically used this approach for deep and unbiased analysis of the effects of a drug library on five cultured cell lines. The presented method enables the acquisition and analysis of millions of images, of treated and control cells, followed by an automated identification of drugs inducing strong responses, evaluating the median effect concentrations and those cellular properties that are most highly affected by the drug. The tools described here provide standardized quantification of multiple attributes for systems level dissection of complex functions in normal and diseased cells, using multiple perturbations. Such analysis of cells, derived from pathological samples, may help in the diagnosis and follow-up of treatment in patients. |
| first_indexed | 2024-12-13T13:28:09Z |
| format | Article |
| id | doaj.art-ff9be6918dd946ee8d973f227bc4c906 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-13T13:28:09Z |
| publishDate | 2020-10-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-ff9be6918dd946ee8d973f227bc4c9062022-12-21T23:44:15ZengF1000 Research LtdF1000Research2046-14022020-10-01910.12688/f1000research.26254.128977Multi-parametric characterization of drug effects on cells [version 1; peer review: 2 approved]Yael Paran0Yuvalal Liron1Sarit Batsir2Nicola Mabjeesh3Benjamin Geiger4Zvi Kam5IDEA Biomedical Ltd., Rehovot, 76705, IsraelDepartment of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, IsraelDepartment of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, IsraelDepartment of Urology, Tel Aviv Sourasky Medical Center, Tel Aviv, 64239, IsraelDepartment of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, IsraelDepartment of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, IsraelWe present here a novel multi-parametric approach for the characterization of multiple cellular features, using images acquired by high-throughput and high-definition light microscopy. We specifically used this approach for deep and unbiased analysis of the effects of a drug library on five cultured cell lines. The presented method enables the acquisition and analysis of millions of images, of treated and control cells, followed by an automated identification of drugs inducing strong responses, evaluating the median effect concentrations and those cellular properties that are most highly affected by the drug. The tools described here provide standardized quantification of multiple attributes for systems level dissection of complex functions in normal and diseased cells, using multiple perturbations. Such analysis of cells, derived from pathological samples, may help in the diagnosis and follow-up of treatment in patients.https://f1000research.com/articles/9-1199/v1 |
| spellingShingle | Yael Paran Yuvalal Liron Sarit Batsir Nicola Mabjeesh Benjamin Geiger Zvi Kam Multi-parametric characterization of drug effects on cells [version 1; peer review: 2 approved] F1000Research |
| title | Multi-parametric characterization of drug effects on cells [version 1; peer review: 2 approved] |
| title_full | Multi-parametric characterization of drug effects on cells [version 1; peer review: 2 approved] |
| title_fullStr | Multi-parametric characterization of drug effects on cells [version 1; peer review: 2 approved] |
| title_full_unstemmed | Multi-parametric characterization of drug effects on cells [version 1; peer review: 2 approved] |
| title_short | Multi-parametric characterization of drug effects on cells [version 1; peer review: 2 approved] |
| title_sort | multi parametric characterization of drug effects on cells version 1 peer review 2 approved |
| url | https://f1000research.com/articles/9-1199/v1 |
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