The effect of heterologous seminal plasma from ram, buck or camel on the freezability of ram semen

Here, we compared the effects of ram, buck and dromedary camel seminal plasma mixed with TRIS-egg yolk glycerol extender on the freezing preservation of ram semen. Awassi ram semen samples underwent primary evaluation and were then pooled and diluted with the following diluents: TRIS-egg yolk glycer...

Full description

Bibliographic Details
Main Authors: A.A. Swelum, I.M. Saadeldin, M. Bahadi, M. Afifi, M. Al-Mutary, A.N. Alowaimer
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2018-11-01
Series:Veterinární Medicína
Subjects:
Online Access:https://vetmed.agriculturejournals.cz/artkey/vet-201811-0002_the-effect-of-heterologous-seminal-plasma-from-ram-buck-or-camel-on-the-freezability-of-ram-semen.php
_version_ 1797897487332147200
author A.A. Swelum
I.M. Saadeldin
M. Bahadi
M. Afifi
M. Al-Mutary
A.N. Alowaimer
author_facet A.A. Swelum
I.M. Saadeldin
M. Bahadi
M. Afifi
M. Al-Mutary
A.N. Alowaimer
author_sort A.A. Swelum
collection DOAJ
description Here, we compared the effects of ram, buck and dromedary camel seminal plasma mixed with TRIS-egg yolk glycerol extender on the freezing preservation of ram semen. Awassi ram semen samples underwent primary evaluation and were then pooled and diluted with the following diluents: TRIS-egg yolk glycerol mixed with (1) whole ram semen as a control (T); (2) ram sperm after seminal plasma removal (W); or (3) ram, (4) buck or (5) camel seminal plasma (R, B and C, respectively). The diluted semen was frozen using liquid nitrogen vapor. Various sperm parameters were evaluated in the frozen semen. Total motility before and after freezing was significantly higher in R, B and C diluents than in T and W diluents. Progressive motility after freezing was significantly higher in R, B, C and T diluents than in W diluent. Vitality after freezing was significantly higher in B than in W diluent. DNA fragmentation before and after freezing was significantly lower in R, B, C and T diluents than in W diluent. Plasma membrane integrity before and after freezing was significantly higher in R, B and C diluents than in W diluent. Sperm abnormalities before freezing were significantly lower in R, B and C diluents than in W diluent. Malondialdehyde concentration was significantly higher in T and W diluents than other diluents. Reduced glutathione concentration was significantly higher in B diluent than other diluents. Moreover, reduced glutathione concentration was significantly higher in C, R and W diluents than in T diluent. Thus, the addition of ram, buck or camel seminal plasma to TRIS-egg yolk glycerol extender improved the quality of frozen ram semen, while seminal plasma removal adversely affected it. Ram, buck and camel seminal plasma had similar effects, with no significant differences between them on the evaluated parameters of frozen ram semen.
first_indexed 2024-04-10T07:59:13Z
format Article
id doaj.art-ff9cfbadeaa548559c8aa956d58fb528
institution Directory Open Access Journal
issn 0375-8427
1805-9392
language English
last_indexed 2024-04-10T07:59:13Z
publishDate 2018-11-01
publisher Czech Academy of Agricultural Sciences
record_format Article
series Veterinární Medicína
spelling doaj.art-ff9cfbadeaa548559c8aa956d58fb5282023-02-23T03:50:47ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922018-11-01631150051210.17221/52/2018-VETMEDvet-201811-0002The effect of heterologous seminal plasma from ram, buck or camel on the freezability of ram semenA.A. Swelum0I.M. Saadeldin1M. Bahadi2M. Afifi3M. Al-Mutary4A.N. Alowaimer5College of Food and Agriculture Sciences, King Saud University, Riyadh, Kingdom of Saudi ArabiaCollege of Food and Agriculture Sciences, King Saud University, Riyadh, Kingdom of Saudi ArabiaCollege of Food and Agriculture Sciences, King Saud University, Riyadh, Kingdom of Saudi ArabiaFaculty of Veterinary Medicine, Zagazig University, Zagazig, EgyptCollege of Education, Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi ArabiaCollege of Food and Agriculture Sciences, King Saud University, Riyadh, Kingdom of Saudi ArabiaHere, we compared the effects of ram, buck and dromedary camel seminal plasma mixed with TRIS-egg yolk glycerol extender on the freezing preservation of ram semen. Awassi ram semen samples underwent primary evaluation and were then pooled and diluted with the following diluents: TRIS-egg yolk glycerol mixed with (1) whole ram semen as a control (T); (2) ram sperm after seminal plasma removal (W); or (3) ram, (4) buck or (5) camel seminal plasma (R, B and C, respectively). The diluted semen was frozen using liquid nitrogen vapor. Various sperm parameters were evaluated in the frozen semen. Total motility before and after freezing was significantly higher in R, B and C diluents than in T and W diluents. Progressive motility after freezing was significantly higher in R, B, C and T diluents than in W diluent. Vitality after freezing was significantly higher in B than in W diluent. DNA fragmentation before and after freezing was significantly lower in R, B, C and T diluents than in W diluent. Plasma membrane integrity before and after freezing was significantly higher in R, B and C diluents than in W diluent. Sperm abnormalities before freezing were significantly lower in R, B and C diluents than in W diluent. Malondialdehyde concentration was significantly higher in T and W diluents than other diluents. Reduced glutathione concentration was significantly higher in B diluent than other diluents. Moreover, reduced glutathione concentration was significantly higher in C, R and W diluents than in T diluent. Thus, the addition of ram, buck or camel seminal plasma to TRIS-egg yolk glycerol extender improved the quality of frozen ram semen, while seminal plasma removal adversely affected it. Ram, buck and camel seminal plasma had similar effects, with no significant differences between them on the evaluated parameters of frozen ram semen.https://vetmed.agriculturejournals.cz/artkey/vet-201811-0002_the-effect-of-heterologous-seminal-plasma-from-ram-buck-or-camel-on-the-freezability-of-ram-semen.phpspermextendercryopreservationsheepgoatpost-thawingtris
spellingShingle A.A. Swelum
I.M. Saadeldin
M. Bahadi
M. Afifi
M. Al-Mutary
A.N. Alowaimer
The effect of heterologous seminal plasma from ram, buck or camel on the freezability of ram semen
Veterinární Medicína
sperm
extender
cryopreservation
sheep
goat
post-thawing
tris
title The effect of heterologous seminal plasma from ram, buck or camel on the freezability of ram semen
title_full The effect of heterologous seminal plasma from ram, buck or camel on the freezability of ram semen
title_fullStr The effect of heterologous seminal plasma from ram, buck or camel on the freezability of ram semen
title_full_unstemmed The effect of heterologous seminal plasma from ram, buck or camel on the freezability of ram semen
title_short The effect of heterologous seminal plasma from ram, buck or camel on the freezability of ram semen
title_sort effect of heterologous seminal plasma from ram buck or camel on the freezability of ram semen
topic sperm
extender
cryopreservation
sheep
goat
post-thawing
tris
url https://vetmed.agriculturejournals.cz/artkey/vet-201811-0002_the-effect-of-heterologous-seminal-plasma-from-ram-buck-or-camel-on-the-freezability-of-ram-semen.php
work_keys_str_mv AT aaswelum theeffectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen
AT imsaadeldin theeffectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen
AT mbahadi theeffectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen
AT mafifi theeffectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen
AT malmutary theeffectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen
AT analowaimer theeffectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen
AT aaswelum effectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen
AT imsaadeldin effectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen
AT mbahadi effectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen
AT mafifi effectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen
AT malmutary effectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen
AT analowaimer effectofheterologousseminalplasmafromrambuckorcamelonthefreezabilityoframsemen