Bioconversion of Some Agro-Residues into Organic Acids by Cellulolytic Rock-Phosphate-Solubilizing <i>Aspergillus japonicus</i>
Biological-based conversion of agricultural residues into bioactive compounds may be considered to be the basis for various vital industries. However, finding a suitable microorganism is a challenge in the bioconversion process. Therefore, this study was conducted to find local fungal isolates able...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/9/437 |
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| author | Samir A. Mahgoub Elmadawy G. A. Kedra Hassan I. Abdelfattah Howaida M. Abdelbasit Soha A. Alamoudi Diana A. Al-Quwaie Samy Selim Salam S. Alsharari Wesam I. A. Saber Rasha M. El-Mekkawy |
| author_facet | Samir A. Mahgoub Elmadawy G. A. Kedra Hassan I. Abdelfattah Howaida M. Abdelbasit Soha A. Alamoudi Diana A. Al-Quwaie Samy Selim Salam S. Alsharari Wesam I. A. Saber Rasha M. El-Mekkawy |
| author_sort | Samir A. Mahgoub |
| collection | DOAJ |
| description | Biological-based conversion of agricultural residues into bioactive compounds may be considered to be the basis for various vital industries. However, finding a suitable microorganism is a challenge in the bioconversion process. Therefore, this study was conducted to find local fungal isolates able to convert a combination of plant biomass residues into organic acids (OAs). Based on their cellulase and phytase activities and rock phosphate (RP) solubilization potential, an efficient 15 fungal isolates (named F1 to F15) were selected and identified by both morphological and molecular methods using the 18S rRNA sequencing technique. The best fungal isolate (F15) was identified as <i>Aspergillus japonicus</i>. After 4 weeks of incubation below solid-state fermentation (SSF) with a mix of sugarcane bagasse and faba bean straw (3:7), with 7.5% (<i>v</i>/<i>w</i>) fungal inoculum to the growth medium, the biodegradation process by the fungus reached its peak, i.e., maximum cellulolytic activity and RP solubilization ability. Under such fermentation conditions, seven organic acids were detected using HPLC, in the following order: ascorbic acid > oxalic acid > formic acid > malic acid > succinic acid > lactic acid > citric acid. Based on the results, <i>Aspergillus japonicus</i> (F15) could produce OAs and cellulose enzymes, and could be considered a new single-step bio-converter of sugarcane bagasse and faba bean straw residues into OAs. Furthermore, this fungus could be a new source of fungal cellulose, and could present a practical approach to reducing environmental contamination. Additional work is encouraged for more optimization of fermentation conditions. |
| first_indexed | 2024-03-10T00:04:52Z |
| format | Article |
| id | doaj.art-ff9fdb2244bf4d3db784b00fcb7e2a0c |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T00:04:52Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-ff9fdb2244bf4d3db784b00fcb7e2a0c2023-11-23T16:10:40ZengMDPI AGFermentation2311-56372022-09-018943710.3390/fermentation8090437Bioconversion of Some Agro-Residues into Organic Acids by Cellulolytic Rock-Phosphate-Solubilizing <i>Aspergillus japonicus</i>Samir A. Mahgoub0Elmadawy G. A. Kedra1Hassan I. Abdelfattah2Howaida M. Abdelbasit3Soha A. Alamoudi4Diana A. Al-Quwaie5Samy Selim6Salam S. Alsharari7Wesam I. A. Saber8Rasha M. El-Mekkawy9Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig 44511, EgyptDepartment of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig 44511, EgyptDepartment of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig 44511, EgyptDepartment of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig 44511, EgyptBiological Sciences Department, College of Science & Arts, King Abdulaziz University, Rabigh 21911, Saudi ArabiaBiological Sciences Department, College of Science & Arts, King Abdulaziz University, Rabigh 21911, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72388, Saudi ArabiaBiology Department, College of Science, Jouf University, Sakaka 72446, Saudi ArabiaMicrobial Activity Unit, Department of Microbiology, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12619, EgyptDepartment of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig 44511, EgyptBiological-based conversion of agricultural residues into bioactive compounds may be considered to be the basis for various vital industries. However, finding a suitable microorganism is a challenge in the bioconversion process. Therefore, this study was conducted to find local fungal isolates able to convert a combination of plant biomass residues into organic acids (OAs). Based on their cellulase and phytase activities and rock phosphate (RP) solubilization potential, an efficient 15 fungal isolates (named F1 to F15) were selected and identified by both morphological and molecular methods using the 18S rRNA sequencing technique. The best fungal isolate (F15) was identified as <i>Aspergillus japonicus</i>. After 4 weeks of incubation below solid-state fermentation (SSF) with a mix of sugarcane bagasse and faba bean straw (3:7), with 7.5% (<i>v</i>/<i>w</i>) fungal inoculum to the growth medium, the biodegradation process by the fungus reached its peak, i.e., maximum cellulolytic activity and RP solubilization ability. Under such fermentation conditions, seven organic acids were detected using HPLC, in the following order: ascorbic acid > oxalic acid > formic acid > malic acid > succinic acid > lactic acid > citric acid. Based on the results, <i>Aspergillus japonicus</i> (F15) could produce OAs and cellulose enzymes, and could be considered a new single-step bio-converter of sugarcane bagasse and faba bean straw residues into OAs. Furthermore, this fungus could be a new source of fungal cellulose, and could present a practical approach to reducing environmental contamination. Additional work is encouraged for more optimization of fermentation conditions.https://www.mdpi.com/2311-5637/8/9/437rock phosphate solubilizationcellulasessugarcane bagassefaba bean strawphytase |
| spellingShingle | Samir A. Mahgoub Elmadawy G. A. Kedra Hassan I. Abdelfattah Howaida M. Abdelbasit Soha A. Alamoudi Diana A. Al-Quwaie Samy Selim Salam S. Alsharari Wesam I. A. Saber Rasha M. El-Mekkawy Bioconversion of Some Agro-Residues into Organic Acids by Cellulolytic Rock-Phosphate-Solubilizing <i>Aspergillus japonicus</i> Fermentation rock phosphate solubilization cellulases sugarcane bagasse faba bean straw phytase |
| title | Bioconversion of Some Agro-Residues into Organic Acids by Cellulolytic Rock-Phosphate-Solubilizing <i>Aspergillus japonicus</i> |
| title_full | Bioconversion of Some Agro-Residues into Organic Acids by Cellulolytic Rock-Phosphate-Solubilizing <i>Aspergillus japonicus</i> |
| title_fullStr | Bioconversion of Some Agro-Residues into Organic Acids by Cellulolytic Rock-Phosphate-Solubilizing <i>Aspergillus japonicus</i> |
| title_full_unstemmed | Bioconversion of Some Agro-Residues into Organic Acids by Cellulolytic Rock-Phosphate-Solubilizing <i>Aspergillus japonicus</i> |
| title_short | Bioconversion of Some Agro-Residues into Organic Acids by Cellulolytic Rock-Phosphate-Solubilizing <i>Aspergillus japonicus</i> |
| title_sort | bioconversion of some agro residues into organic acids by cellulolytic rock phosphate solubilizing i aspergillus japonicus i |
| topic | rock phosphate solubilization cellulases sugarcane bagasse faba bean straw phytase |
| url | https://www.mdpi.com/2311-5637/8/9/437 |
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