Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells
The potential applications of human embryonic stem cells (hESCs) in regenerative medicine and developmental research have made stem cell biology one of the most fascinating and rapidly expanding fields of biomedicine. The first clinical trial of hESCs in humans has begun, and the field of stem cell...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320415000085 |
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| author | Hidenori Akutsu Masakazu Machida Seiichi Kanzaki Tohru Sugawara Takashi Ohkura Naoko Nakamura Mayu Yamazaki-Inoue Takumi Miura Mohan C. Vemuri Mahendra S. Rao Kenji Miyado Akihiro Umezawa |
| author_facet | Hidenori Akutsu Masakazu Machida Seiichi Kanzaki Tohru Sugawara Takashi Ohkura Naoko Nakamura Mayu Yamazaki-Inoue Takumi Miura Mohan C. Vemuri Mahendra S. Rao Kenji Miyado Akihiro Umezawa |
| author_sort | Hidenori Akutsu |
| collection | DOAJ |
| description | The potential applications of human embryonic stem cells (hESCs) in regenerative medicine and developmental research have made stem cell biology one of the most fascinating and rapidly expanding fields of biomedicine. The first clinical trial of hESCs in humans has begun, and the field of stem cell therapy has just entered a new era. Here, we report seven hESC lines (SEES-1, -2, -3, -4, -5, -6, and -7). Four of them were derived and maintained on irradiated human mesenchymal stem cells (hMSCs) grown in xenogeneic-free defined media and substrate. Xenogeneic-free hMSCs isolated from the subcutaneous tissue of extra fingers from individuals with polydactyly showed appropriate potentials as feeder layers in the pluripotency and growth of hESCs. In this report, we describe a comprehensive characterization of these newly derived SEES cell lines. In addition, we developed a scalable culture system for hESCs having high biological safety by using gamma-irradiated serum replacement and pharmaceutical-grade recombinant basic fibroblast growth factor (bFGF, also known as trafermin). This is first report describing the maintenance of hESC pluripotency using pharmaceutical-grade human recombinant bFGF (trafermin) and gamma-irradiated serum replacement. Our defined medium system provides a path to scalability in Good Manufacturing Practice (GMP) settings for the generation of clinically relevant cell types from pluripotent cells for therapeutic applications. |
| first_indexed | 2024-12-10T20:21:46Z |
| format | Article |
| id | doaj.art-ff9fe3a75f5746b88b5dd394039fb3d7 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-12-10T20:21:46Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-ff9fe3a75f5746b88b5dd394039fb3d72022-12-22T01:35:01ZengElsevierRegenerative Therapy2352-32042015-06-011C182910.1016/j.reth.2014.12.004Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cellsHidenori Akutsu0Masakazu Machida1Seiichi Kanzaki2Tohru Sugawara3Takashi Ohkura4Naoko Nakamura5Mayu Yamazaki-Inoue6Takumi Miura7Mohan C. Vemuri8Mahendra S. Rao9Kenji Miyado10Akihiro Umezawa11Department of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, JapanThermo Fisher Scientific, 7335 Executive Way, Frederick, MD 21702, USACenter for Regenerative Medicine, National Institutes of Health, Bethesda, MD 20892, USADepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, JapanThe potential applications of human embryonic stem cells (hESCs) in regenerative medicine and developmental research have made stem cell biology one of the most fascinating and rapidly expanding fields of biomedicine. The first clinical trial of hESCs in humans has begun, and the field of stem cell therapy has just entered a new era. Here, we report seven hESC lines (SEES-1, -2, -3, -4, -5, -6, and -7). Four of them were derived and maintained on irradiated human mesenchymal stem cells (hMSCs) grown in xenogeneic-free defined media and substrate. Xenogeneic-free hMSCs isolated from the subcutaneous tissue of extra fingers from individuals with polydactyly showed appropriate potentials as feeder layers in the pluripotency and growth of hESCs. In this report, we describe a comprehensive characterization of these newly derived SEES cell lines. In addition, we developed a scalable culture system for hESCs having high biological safety by using gamma-irradiated serum replacement and pharmaceutical-grade recombinant basic fibroblast growth factor (bFGF, also known as trafermin). This is first report describing the maintenance of hESC pluripotency using pharmaceutical-grade human recombinant bFGF (trafermin) and gamma-irradiated serum replacement. Our defined medium system provides a path to scalability in Good Manufacturing Practice (GMP) settings for the generation of clinically relevant cell types from pluripotent cells for therapeutic applications.http://www.sciencedirect.com/science/article/pii/S2352320415000085Human embryonic stem cellsXenogeneic-free mediumStem cell expansionHuman feeder layerMesenchymal stem cellsGamma irradiation |
| spellingShingle | Hidenori Akutsu Masakazu Machida Seiichi Kanzaki Tohru Sugawara Takashi Ohkura Naoko Nakamura Mayu Yamazaki-Inoue Takumi Miura Mohan C. Vemuri Mahendra S. Rao Kenji Miyado Akihiro Umezawa Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells Regenerative Therapy Human embryonic stem cells Xenogeneic-free medium Stem cell expansion Human feeder layer Mesenchymal stem cells Gamma irradiation |
| title | Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells |
| title_full | Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells |
| title_fullStr | Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells |
| title_full_unstemmed | Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells |
| title_short | Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells |
| title_sort | xenogeneic free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells |
| topic | Human embryonic stem cells Xenogeneic-free medium Stem cell expansion Human feeder layer Mesenchymal stem cells Gamma irradiation |
| url | http://www.sciencedirect.com/science/article/pii/S2352320415000085 |
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