A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles
This study describes a user-friendly and rapid detection system of oral bacteria in the liquid phase for point of care testing based on magnetic immunoassay. We focused on the dependence of the strength of external magnetic field required to switch the magnetic moments of nanoparticles bound to bact...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC
2019-12-01
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| Series: | AIP Advances |
| Online Access: | http://dx.doi.org/10.1063/1.5130437 |
| _version_ | 1818250246849822720 |
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| author | Loi Tonthat Shunnosuke Takahashi Hidehiko Onodera Kazuhiko Okita Shin Yabukami Kotone Yokota Maiko Furuya Hiroyasu Kanetaka Yoshinori Miura Hideki Takahashi Yoshihiko Watanabe Ritsuko Akiyama |
| author_facet | Loi Tonthat Shunnosuke Takahashi Hidehiko Onodera Kazuhiko Okita Shin Yabukami Kotone Yokota Maiko Furuya Hiroyasu Kanetaka Yoshinori Miura Hideki Takahashi Yoshihiko Watanabe Ritsuko Akiyama |
| author_sort | Loi Tonthat |
| collection | DOAJ |
| description | This study describes a user-friendly and rapid detection system of oral bacteria in the liquid phase for point of care testing based on magnetic immunoassay. We focused on the dependence of the strength of external magnetic field required to switch the magnetic moments of nanoparticles bound to bacteria on the bacteria concentration. The results obtained indicate that the required field strength increases linearly as a function of log concentration of Porphyromonas gingivalis cultured in the range of 103–109 CFU/mL. Similarly, the required field strengths for Streptococcus mutans and Pseudomonas aeruginosa increase monotonically when their concentrations increase, whereas the required field strength for Escherichia coli decreases monotonically when its concentration increases. We then measured the concentration of Porphyromonas gingivalis in the saliva collected from elderly people in a geriatric health services facility and the results using the developed system had a correlation with those using a commercial bacteria counter. |
| first_indexed | 2024-12-12T15:49:21Z |
| format | Article |
| id | doaj.art-ffa135fe8ca740878a497998df18cedc |
| institution | Directory Open Access Journal |
| issn | 2158-3226 |
| language | English |
| last_indexed | 2024-12-12T15:49:21Z |
| publishDate | 2019-12-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | AIP Advances |
| spelling | doaj.art-ffa135fe8ca740878a497998df18cedc2022-12-22T00:19:41ZengAIP Publishing LLCAIP Advances2158-32262019-12-01912125325125325-410.1063/1.5130437A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticlesLoi Tonthat0Shunnosuke Takahashi1Hidehiko Onodera2Kazuhiko Okita3Shin Yabukami4Kotone Yokota5Maiko Furuya6Hiroyasu Kanetaka7Yoshinori Miura8Hideki Takahashi9Yoshihiko Watanabe10Ritsuko Akiyama11Department of Electrical Engineering, Tohoku University, Sendai 980-8579, JapanDepartment of Electrical Engineering, Tohoku University, Sendai 980-8579, JapanTohoku Gakuin University, Tagajo 985-853, JapanTohoku Gakuin University, Tagajo 985-853, JapanDepartment of Electrical Engineering, Tohoku University, Sendai 980-8579, JapanDepartment of Electrical Engineering, Tohoku University, Sendai 980-8579, JapanDepartment of Electrical Engineering, Tohoku University, Sendai 980-8579, JapanDepartment of Electrical Engineering, Tohoku University, Sendai 980-8579, JapanJNS Co., Ltd., Natori 981-1251, JapanJNS Co., Ltd., Natori 981-1251, JapanDepartment of Industrial Welfare Management, Tohoku Fukushi University, Sendai 981-8522, JapanSendan No Oka, Sendai 989-3201, JapanThis study describes a user-friendly and rapid detection system of oral bacteria in the liquid phase for point of care testing based on magnetic immunoassay. We focused on the dependence of the strength of external magnetic field required to switch the magnetic moments of nanoparticles bound to bacteria on the bacteria concentration. The results obtained indicate that the required field strength increases linearly as a function of log concentration of Porphyromonas gingivalis cultured in the range of 103–109 CFU/mL. Similarly, the required field strengths for Streptococcus mutans and Pseudomonas aeruginosa increase monotonically when their concentrations increase, whereas the required field strength for Escherichia coli decreases monotonically when its concentration increases. We then measured the concentration of Porphyromonas gingivalis in the saliva collected from elderly people in a geriatric health services facility and the results using the developed system had a correlation with those using a commercial bacteria counter.http://dx.doi.org/10.1063/1.5130437 |
| spellingShingle | Loi Tonthat Shunnosuke Takahashi Hidehiko Onodera Kazuhiko Okita Shin Yabukami Kotone Yokota Maiko Furuya Hiroyasu Kanetaka Yoshinori Miura Hideki Takahashi Yoshihiko Watanabe Ritsuko Akiyama A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles AIP Advances |
| title | A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles |
| title_full | A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles |
| title_fullStr | A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles |
| title_full_unstemmed | A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles |
| title_short | A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles |
| title_sort | simple and rapid detection system for oral bacteria in liquid phase for point of care diagnostics using magnetic nanoparticles |
| url | http://dx.doi.org/10.1063/1.5130437 |
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