Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells
Many aptamer-fluorophore complexes suffer from low quantum yield and low extinction coefficients limiting their usability. Here the authors isolate new Mango aptamers with improved fluorescent properties for both fixed and live-cell imaging by using competitive ligand binding microfluidic selection.
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2018-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-018-02993-8 |
| _version_ | 1830282758914572288 |
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| author | Alexis Autour Sunny C. Y. Jeng Adam D. Cawte Amir Abdolahzadeh Angela Galli Shanker S. S. Panchapakesan David Rueda Michael Ryckelynck Peter J. Unrau |
| author_facet | Alexis Autour Sunny C. Y. Jeng Adam D. Cawte Amir Abdolahzadeh Angela Galli Shanker S. S. Panchapakesan David Rueda Michael Ryckelynck Peter J. Unrau |
| author_sort | Alexis Autour |
| collection | DOAJ |
| description | Many aptamer-fluorophore complexes suffer from low quantum yield and low extinction coefficients limiting their usability. Here the authors isolate new Mango aptamers with improved fluorescent properties for both fixed and live-cell imaging by using competitive ligand binding microfluidic selection. |
| first_indexed | 2024-12-19T02:44:28Z |
| format | Article |
| id | doaj.art-ffa23cd158524e5b94c7284e2e879efb |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T02:44:28Z |
| publishDate | 2018-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-ffa23cd158524e5b94c7284e2e879efb2022-12-21T20:39:00ZengNature PortfolioNature Communications2041-17232018-02-019111210.1038/s41467-018-02993-8Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cellsAlexis Autour0Sunny C. Y. Jeng1Adam D. Cawte2Amir Abdolahzadeh3Angela Galli4Shanker S. S. Panchapakesan5David Rueda6Michael Ryckelynck7Peter J. Unrau8Université de Strasbourg, CNRS, Architecture et Réactivité de l’ARN, UPR 9002Department of Molecular Biology and Biochemistry, Simon Fraser UniversitySingle Molecule Imaging Group, MRC London Institute of Medical SciencesDepartment of Molecular Biology and Biochemistry, Simon Fraser UniversityDepartment of Molecular Biology and Biochemistry, Simon Fraser UniversityDepartment of Molecular Biology and Biochemistry, Simon Fraser UniversitySingle Molecule Imaging Group, MRC London Institute of Medical SciencesUniversité de Strasbourg, CNRS, Architecture et Réactivité de l’ARN, UPR 9002Department of Molecular Biology and Biochemistry, Simon Fraser UniversityMany aptamer-fluorophore complexes suffer from low quantum yield and low extinction coefficients limiting their usability. Here the authors isolate new Mango aptamers with improved fluorescent properties for both fixed and live-cell imaging by using competitive ligand binding microfluidic selection.https://doi.org/10.1038/s41467-018-02993-8 |
| spellingShingle | Alexis Autour Sunny C. Y. Jeng Adam D. Cawte Amir Abdolahzadeh Angela Galli Shanker S. S. Panchapakesan David Rueda Michael Ryckelynck Peter J. Unrau Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells Nature Communications |
| title | Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells |
| title_full | Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells |
| title_fullStr | Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells |
| title_full_unstemmed | Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells |
| title_short | Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells |
| title_sort | fluorogenic rna mango aptamers for imaging small non coding rnas in mammalian cells |
| url | https://doi.org/10.1038/s41467-018-02993-8 |
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