Characteristics of Hepatitis B Virus Genotype and Sub-Genotype in Hepatocellular Cancer Patients in Vietnam
Untreated chronic hepatitis B virus (HBV) infection can lead to chronic liver disease and may progress to cirrhosis or hepatocellular carcinoma (HCC). HBV infection has been prevalent in Vietnam, but there is little information available on the genotypes, sub-genotypes, and mutations of HBV in patie...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/12/10/2393 |
| _version_ | 1827650714212499456 |
|---|---|
| author | Phat Tan Ho Mario Giosuè Balzanelli Pietro Distratis Rita Lazzaro Duy Khanh Tran Kieu C. D. Nguyen Tri Minh Bui Thinh Tien Nguyen Son Truong Pham Huy Song Dinh Nguyen Vinh Thanh Tran Toan Trong Ho Gianna Dipalma Francesco Inchingolo Camelia Quek Huong Thien Pham Ciro Gargiulo Isacco Luigi Santacroce Van Hung Pham |
| author_facet | Phat Tan Ho Mario Giosuè Balzanelli Pietro Distratis Rita Lazzaro Duy Khanh Tran Kieu C. D. Nguyen Tri Minh Bui Thinh Tien Nguyen Son Truong Pham Huy Song Dinh Nguyen Vinh Thanh Tran Toan Trong Ho Gianna Dipalma Francesco Inchingolo Camelia Quek Huong Thien Pham Ciro Gargiulo Isacco Luigi Santacroce Van Hung Pham |
| author_sort | Phat Tan Ho |
| collection | DOAJ |
| description | Untreated chronic hepatitis B virus (HBV) infection can lead to chronic liver disease and may progress to cirrhosis or hepatocellular carcinoma (HCC). HBV infection has been prevalent in Vietnam, but there is little information available on the genotypes, sub-genotypes, and mutations of HBV in patients with HBV-related HCC confirmed by histopathological diagnosis. We studied the molecular characteristics of HBV and its genetic variants in Vietnamese HCC patients after liver tumor resection. We conducted a descriptive cross-sectional study on 107 HBV-related HCC hospitalized patients from October 2018 to April 2019. The specimens collected included EDTA anticoagulant blood and liver tissues. Extracted HBV DNA was subjected to whole genome sequencing by the Sanger method. We discovered 62 individuals (57.9%) with genotype B and 45 patients (42.1%) with genotype C, with only sub-genotypes B4 and C1. Among the mutations, the double mutation, A1762T-G1764A, had the most significant frequency (73/107 samples; 68.2%) and was higher in genotype C than in genotype B (<i>p</i> < 0.001). The most common genotypes found in HCC patients in this investigation were B and C, with sub-genotypes B4 and C1 for each. The prevalence of genotype B4 was greater in HBV-infected Vietnamese HCC patients. |
| first_indexed | 2024-03-09T20:22:54Z |
| format | Article |
| id | doaj.art-ffa4dab861314bef8d02d6fff9018bc5 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T20:22:54Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-ffa4dab861314bef8d02d6fff9018bc52023-11-23T23:44:28ZengMDPI AGDiagnostics2075-44182022-10-011210239310.3390/diagnostics12102393Characteristics of Hepatitis B Virus Genotype and Sub-Genotype in Hepatocellular Cancer Patients in VietnamPhat Tan Ho0Mario Giosuè Balzanelli1Pietro Distratis2Rita Lazzaro3Duy Khanh Tran4Kieu C. D. Nguyen5Tri Minh Bui6Thinh Tien Nguyen7Son Truong Pham8Huy Song Dinh Nguyen9Vinh Thanh Tran10Toan Trong Ho11Gianna Dipalma12Francesco Inchingolo13Camelia Quek14Huong Thien Pham15Ciro Gargiulo Isacco16Luigi Santacroce17Van Hung Pham18Cho Ray Hospital Ho Chi Minh Vietnam, Phat Tan Ho, Ho Chi Minh 749000, VietnamSET-118, Department of Pre-Hospital and Emergency, SG Giuseppe Moscati Hospital, 74100 Taranto, ItalySET-118, Department of Pre-Hospital and Emergency, SG Giuseppe Moscati Hospital, 74100 Taranto, ItalySET-118, Department of Pre-Hospital and Emergency, SG Giuseppe Moscati Hospital, 74100 Taranto, ItalyNam Khoa Biotek, Ho Chi Minh 500000, VietnamAmerican Stem Cells Hospital Ho Chi Minh, Ho Chi Minh 700000, VietnamNam Khoa Biotek, Ho Chi Minh 500000, Vietnam108 Military Central Hospital, Hanoi 100000, VietnamWestern Sydney Local Health District, Sydney 2170, AustraliaCho Ray Hospital Ho Chi Minh Vietnam, Phat Tan Ho, Ho Chi Minh 749000, VietnamCho Ray Hospital Ho Chi Minh Vietnam, Phat Tan Ho, Ho Chi Minh 749000, VietnamCho Ray Hospital Ho Chi Minh Vietnam, Phat Tan Ho, Ho Chi Minh 749000, VietnamSchool of Medicine, University of Bari “Aldo Moro”, 70124 Bari, ItalySchool of Medicine, University of Bari “Aldo Moro”, 70124 Bari, ItalyFaculty of Medicine and Health, The University of Sydney, Sydney 2050, AustraliaMultidisciplinary Clinic, University of Medicine Pham Ngoc Thach, Ho Chi Minh 700000, VietnamSET-118, Department of Pre-Hospital and Emergency, SG Giuseppe Moscati Hospital, 74100 Taranto, ItalyInterdisciplinary Department of Medicine, Microbiology and Virology Unit, School of Medicine, University of Bari “Aldo Moro”, 70124 Bari, ItalyNam Khoa Biotek, Ho Chi Minh 500000, VietnamUntreated chronic hepatitis B virus (HBV) infection can lead to chronic liver disease and may progress to cirrhosis or hepatocellular carcinoma (HCC). HBV infection has been prevalent in Vietnam, but there is little information available on the genotypes, sub-genotypes, and mutations of HBV in patients with HBV-related HCC confirmed by histopathological diagnosis. We studied the molecular characteristics of HBV and its genetic variants in Vietnamese HCC patients after liver tumor resection. We conducted a descriptive cross-sectional study on 107 HBV-related HCC hospitalized patients from October 2018 to April 2019. The specimens collected included EDTA anticoagulant blood and liver tissues. Extracted HBV DNA was subjected to whole genome sequencing by the Sanger method. We discovered 62 individuals (57.9%) with genotype B and 45 patients (42.1%) with genotype C, with only sub-genotypes B4 and C1. Among the mutations, the double mutation, A1762T-G1764A, had the most significant frequency (73/107 samples; 68.2%) and was higher in genotype C than in genotype B (<i>p</i> < 0.001). The most common genotypes found in HCC patients in this investigation were B and C, with sub-genotypes B4 and C1 for each. The prevalence of genotype B4 was greater in HBV-infected Vietnamese HCC patients.https://www.mdpi.com/2075-4418/12/10/2393hepatitis B virusgenotypesub-genotypehepatocellular carcinoma |
| spellingShingle | Phat Tan Ho Mario Giosuè Balzanelli Pietro Distratis Rita Lazzaro Duy Khanh Tran Kieu C. D. Nguyen Tri Minh Bui Thinh Tien Nguyen Son Truong Pham Huy Song Dinh Nguyen Vinh Thanh Tran Toan Trong Ho Gianna Dipalma Francesco Inchingolo Camelia Quek Huong Thien Pham Ciro Gargiulo Isacco Luigi Santacroce Van Hung Pham Characteristics of Hepatitis B Virus Genotype and Sub-Genotype in Hepatocellular Cancer Patients in Vietnam Diagnostics hepatitis B virus genotype sub-genotype hepatocellular carcinoma |
| title | Characteristics of Hepatitis B Virus Genotype and Sub-Genotype in Hepatocellular Cancer Patients in Vietnam |
| title_full | Characteristics of Hepatitis B Virus Genotype and Sub-Genotype in Hepatocellular Cancer Patients in Vietnam |
| title_fullStr | Characteristics of Hepatitis B Virus Genotype and Sub-Genotype in Hepatocellular Cancer Patients in Vietnam |
| title_full_unstemmed | Characteristics of Hepatitis B Virus Genotype and Sub-Genotype in Hepatocellular Cancer Patients in Vietnam |
| title_short | Characteristics of Hepatitis B Virus Genotype and Sub-Genotype in Hepatocellular Cancer Patients in Vietnam |
| title_sort | characteristics of hepatitis b virus genotype and sub genotype in hepatocellular cancer patients in vietnam |
| topic | hepatitis B virus genotype sub-genotype hepatocellular carcinoma |
| url | https://www.mdpi.com/2075-4418/12/10/2393 |
| work_keys_str_mv | AT phattanho characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT mariogiosuebalzanelli characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT pietrodistratis characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT ritalazzaro characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT duykhanhtran characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT kieucdnguyen characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT triminhbui characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT thinhtiennguyen characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT sontruongpham characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT huysongdinhnguyen characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT vinhthanhtran characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT toantrongho characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT giannadipalma characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT francescoinchingolo characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT cameliaquek characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT huongthienpham characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT cirogargiuloisacco characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT luigisantacroce characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam AT vanhungpham characteristicsofhepatitisbvirusgenotypeandsubgenotypeinhepatocellularcancerpatientsinvietnam |