Genotyping of C and F Regions of Plasmodium Falciparum EBA-175 in South-East of Iran
Abstract Background and Objective: The Plasmodium falciparum EBA-175, via Sialic acid dependent glycophorin A, binds to red blood cells and thus plays a critical role in cell invasion. Some part of second allele in its gene encoding in FCR-3 (Section F) and CAMP (Section C) can be found. This study...
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2014-04-01
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| Series: | Medical Laboratory Journal |
| Subjects: | |
| Online Access: | http://mlj.goums.ac.ir/article-1-407-en.html |
| _version_ | 1818156403402997760 |
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| author | A Jamshidi M Mousavi Ghahfarrokhi A Gharaei A Ebrahimzadeh M Jaffari Modrek A Ansari Moghadam S Mohammadi |
| author_facet | A Jamshidi M Mousavi Ghahfarrokhi A Gharaei A Ebrahimzadeh M Jaffari Modrek A Ansari Moghadam S Mohammadi |
| author_sort | A Jamshidi |
| collection | DOAJ |
| description | Abstract
Background and Objective: The Plasmodium falciparum EBA-175, via Sialic acid dependent glycophorin A, binds to red blood cells and thus plays a critical role in cell invasion. Some part of second allele in its gene encoding in FCR-3 (Section F) and CAMP (Section C) can be found. This study aimed to determine the prevalence of Plasmodium falciparum EBA-175KD alleles in southeastern Iran.
Material and Methods: In this cross-sectional study, using polymerase chain reaction Nest (Nested-PCR) with specific primers was used for the two parts of the EBA-175 gene to be proliferated. Ninety–four microscopic positive blood samples from individuals infected by Plasmodium falciparum were obtained from four different locations in southeastern Iran.
Results: Of 94 positive samples, 88 were antigen EBA-175KD. Genotype CAMP (714 bp) and FCR-3 (to 795 bp), respectively, in 31 (32.97 %) and 49 (52.12 %) were found. Eight samples have both FCR-3 and CAMP.
Conclusion: Both of EBA-175KD dimorphic genes were found. The frequency of FCR-3 allele was higher in the South East of Iran. Thus, this pattern can be considered in making Plasmodium falciparum vaccines for this area.
Key words: Plasmodium Falciparum Erythrocyte Binding Antigen-175 South-East of Iran |
| first_indexed | 2024-12-11T14:57:45Z |
| format | Article |
| id | doaj.art-ffa757ce461545f38b19ea406f3a4fc1 |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-12-11T14:57:45Z |
| publishDate | 2014-04-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-ffa757ce461545f38b19ea406f3a4fc12022-12-22T01:01:16ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492014-04-01813641Genotyping of C and F Regions of Plasmodium Falciparum EBA-175 in South-East of IranA Jamshidi0M Mousavi Ghahfarrokhi1A Gharaei2A Ebrahimzadeh3M Jaffari Modrek4A Ansari Moghadam5S Mohammadi6 Abstract Background and Objective: The Plasmodium falciparum EBA-175, via Sialic acid dependent glycophorin A, binds to red blood cells and thus plays a critical role in cell invasion. Some part of second allele in its gene encoding in FCR-3 (Section F) and CAMP (Section C) can be found. This study aimed to determine the prevalence of Plasmodium falciparum EBA-175KD alleles in southeastern Iran. Material and Methods: In this cross-sectional study, using polymerase chain reaction Nest (Nested-PCR) with specific primers was used for the two parts of the EBA-175 gene to be proliferated. Ninety–four microscopic positive blood samples from individuals infected by Plasmodium falciparum were obtained from four different locations in southeastern Iran. Results: Of 94 positive samples, 88 were antigen EBA-175KD. Genotype CAMP (714 bp) and FCR-3 (to 795 bp), respectively, in 31 (32.97 %) and 49 (52.12 %) were found. Eight samples have both FCR-3 and CAMP. Conclusion: Both of EBA-175KD dimorphic genes were found. The frequency of FCR-3 allele was higher in the South East of Iran. Thus, this pattern can be considered in making Plasmodium falciparum vaccines for this area. Key words: Plasmodium Falciparum Erythrocyte Binding Antigen-175 South-East of Iranhttp://mlj.goums.ac.ir/article-1-407-en.htmlplasmodium falciparumerythrocyte binding antigen-175south-east of iran |
| spellingShingle | A Jamshidi M Mousavi Ghahfarrokhi A Gharaei A Ebrahimzadeh M Jaffari Modrek A Ansari Moghadam S Mohammadi Genotyping of C and F Regions of Plasmodium Falciparum EBA-175 in South-East of Iran Medical Laboratory Journal plasmodium falciparum erythrocyte binding antigen-175 south-east of iran |
| title | Genotyping of C and F Regions of Plasmodium Falciparum EBA-175 in South-East of Iran |
| title_full | Genotyping of C and F Regions of Plasmodium Falciparum EBA-175 in South-East of Iran |
| title_fullStr | Genotyping of C and F Regions of Plasmodium Falciparum EBA-175 in South-East of Iran |
| title_full_unstemmed | Genotyping of C and F Regions of Plasmodium Falciparum EBA-175 in South-East of Iran |
| title_short | Genotyping of C and F Regions of Plasmodium Falciparum EBA-175 in South-East of Iran |
| title_sort | genotyping of c and f regions of plasmodium falciparum eba 175 in south east of iran |
| topic | plasmodium falciparum erythrocyte binding antigen-175 south-east of iran |
| url | http://mlj.goums.ac.ir/article-1-407-en.html |
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