A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats
Abstract This study aimed to assess the efficacy of a heat-inactivated Mycobacterium caprae (HIMC) vaccine in goats experimentally challenged with the same strain of M. caprae. Twenty-one goats were divided into three groups of seven: vaccinated with heat-inactivated Mycobacterium bovis (HIMB), with...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-26683-0 |
| _version_ | 1797977512978939904 |
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| author | Cristian Melgarejo Carles Planas Alex Cobos Claudia Arrieta-Villegas Iker A. Sevilla Javier Bezos Xavier Moll Yvonne Espada Joseba M. Garrido Mariano Domingo Enric Vidal Bernat Pérez de Val |
| author_facet | Cristian Melgarejo Carles Planas Alex Cobos Claudia Arrieta-Villegas Iker A. Sevilla Javier Bezos Xavier Moll Yvonne Espada Joseba M. Garrido Mariano Domingo Enric Vidal Bernat Pérez de Val |
| author_sort | Cristian Melgarejo |
| collection | DOAJ |
| description | Abstract This study aimed to assess the efficacy of a heat-inactivated Mycobacterium caprae (HIMC) vaccine in goats experimentally challenged with the same strain of M. caprae. Twenty-one goats were divided into three groups of seven: vaccinated with heat-inactivated Mycobacterium bovis (HIMB), with HIMC and unvaccinated. At 7 weeks post-vaccination all animals were endobronchially challenged with M. caprae. Blood samples were collected for immunological assays and clinical signs were recorded throughout the experiment. All goats were euthanized at 9 weeks post-challenge. Gross pathological examination, analysis of lung pathology using computed tomography, and bacterial load quantification in pulmonary lymph nodes (LN) by qPCR were carried out. Only HIMC vaccinated goats showed a significant reduction of lung lesions volume and mycobacterial DNA load in LN compared to unvaccinated controls. Both vaccinated groups showed also a significant reduction of the other pathological parameters, an improved clinical outcome and a higher proportion of IFN-γ-producing central memory T cells after vaccination. The results indicated that homologous vaccination of goats with HIMC induced enhanced protection against M. caprae challenge by reducing lung pathology and bacterial load compared to the heterologous vaccine (HIMB). Further large-scale trials are necessary to assess the efficacy of autovaccines under field conditions. |
| first_indexed | 2024-04-11T05:09:14Z |
| format | Article |
| id | doaj.art-ffa802fabe15441a9647c7b05075cc04 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T05:09:14Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ffa802fabe15441a9647c7b05075cc042022-12-25T12:12:10ZengNature PortfolioScientific Reports2045-23222022-12-0112111210.1038/s41598-022-26683-0A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goatsCristian Melgarejo0Carles Planas1Alex Cobos2Claudia Arrieta-Villegas3Iker A. Sevilla4Javier Bezos5Xavier Moll6Yvonne Espada7Joseba M. Garrido8Mariano Domingo9Enric Vidal10Bernat Pérez de Val11Unitat Mixta d’investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA)Departament de Medicina i Cirurgía Animals, Universitat Autònoma de BarcelonaUnitat Mixta d’investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA)Unitat Mixta d’investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA)Animal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Basque Research and Technology Alliance (BRTA). DerioDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de MadridDepartament de Medicina i Cirurgía Animals, Universitat Autònoma de BarcelonaDepartament de Medicina i Cirurgía Animals, Universitat Autònoma de BarcelonaAnimal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Basque Research and Technology Alliance (BRTA). DerioUnitat Mixta d’investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA)Unitat Mixta d’investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA)Unitat Mixta d’investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA)Abstract This study aimed to assess the efficacy of a heat-inactivated Mycobacterium caprae (HIMC) vaccine in goats experimentally challenged with the same strain of M. caprae. Twenty-one goats were divided into three groups of seven: vaccinated with heat-inactivated Mycobacterium bovis (HIMB), with HIMC and unvaccinated. At 7 weeks post-vaccination all animals were endobronchially challenged with M. caprae. Blood samples were collected for immunological assays and clinical signs were recorded throughout the experiment. All goats were euthanized at 9 weeks post-challenge. Gross pathological examination, analysis of lung pathology using computed tomography, and bacterial load quantification in pulmonary lymph nodes (LN) by qPCR were carried out. Only HIMC vaccinated goats showed a significant reduction of lung lesions volume and mycobacterial DNA load in LN compared to unvaccinated controls. Both vaccinated groups showed also a significant reduction of the other pathological parameters, an improved clinical outcome and a higher proportion of IFN-γ-producing central memory T cells after vaccination. The results indicated that homologous vaccination of goats with HIMC induced enhanced protection against M. caprae challenge by reducing lung pathology and bacterial load compared to the heterologous vaccine (HIMB). Further large-scale trials are necessary to assess the efficacy of autovaccines under field conditions.https://doi.org/10.1038/s41598-022-26683-0 |
| spellingShingle | Cristian Melgarejo Carles Planas Alex Cobos Claudia Arrieta-Villegas Iker A. Sevilla Javier Bezos Xavier Moll Yvonne Espada Joseba M. Garrido Mariano Domingo Enric Vidal Bernat Pérez de Val A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats Scientific Reports |
| title | A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats |
| title_full | A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats |
| title_fullStr | A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats |
| title_full_unstemmed | A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats |
| title_short | A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats |
| title_sort | proof of concept study to investigate the efficacy of heat inactivated autovaccines in mycobacterium caprae experimentally challenged goats |
| url | https://doi.org/10.1038/s41598-022-26683-0 |
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