Factors affecting vitiligo response to treatment: do MiRNA 196a2C/T gene polymorphism and serum tyrosinase levels have any role?
Background Factors contributing to the pathogenesis of vitiligo and factors affecting its response to treatment are still a major area of debate. Aim of the work The study aimed to assess the serum levels of tyrosinase and Micro-RNAs (miRNAs) gene polymorphism in a sample of Egyptian vitiligo patien...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-04-01
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| Series: | Journal of Dermatological Treatment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09546634.2020.1810202 |
| _version_ | 1797683542278275072 |
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| author | Khaled Mohey El-Din Monib Hanan Hassan Sabry Mohamed Saber Hussein Asmaa Adel El-fallah Rehab Mohammed Salem |
| author_facet | Khaled Mohey El-Din Monib Hanan Hassan Sabry Mohamed Saber Hussein Asmaa Adel El-fallah Rehab Mohammed Salem |
| author_sort | Khaled Mohey El-Din Monib |
| collection | DOAJ |
| description | Background Factors contributing to the pathogenesis of vitiligo and factors affecting its response to treatment are still a major area of debate. Aim of the work The study aimed to assess the serum levels of tyrosinase and Micro-RNAs (miRNAs) gene polymorphism in a sample of Egyptian vitiligo patients, and to determine factors affecting the response of vitiligo to treatment. Subjects and methods This prospective case-control interventional study included 212 non-segmental vitiligo patients and 96 control subjects. Before treatment, vitiligo was evaluated using Vitiligo Area Severity Index. Detection of miRNA 196a-2 polymorphism was done using PCR-REELP and serum tyrosinase was measured using ELISA. After treatment, patients were reevaluated clinically and serum tyrosinase levels were re-measured. Results The tyrosinase levels were significantly elevated in patients. The TT genotype was the most prevalent one in the patients. The percentage of improvement showed a significant positive correlation with patients' ages and age of the disease onset and a negative correlation with disease duration, baseline VASI scores and serum tyrosinase levels. Conclusion MiRNA 196a-2 C/T (11614913) gene polymorphism and the elevated serum tyrosinase levels might be related to the pathogenesis of vitiligo and may affect its therapeutic response. |
| first_indexed | 2024-03-12T00:16:32Z |
| format | Article |
| id | doaj.art-ffa9014f5c10471c9dac1dc92005083f |
| institution | Directory Open Access Journal |
| issn | 0954-6634 1471-1753 |
| language | English |
| last_indexed | 2024-03-12T00:16:32Z |
| publishDate | 2022-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Dermatological Treatment |
| spelling | doaj.art-ffa9014f5c10471c9dac1dc92005083f2023-09-15T14:28:49ZengTaylor & Francis GroupJournal of Dermatological Treatment0954-66341471-17532022-04-013331351135510.1080/09546634.2020.18102021810202Factors affecting vitiligo response to treatment: do MiRNA 196a2C/T gene polymorphism and serum tyrosinase levels have any role?Khaled Mohey El-Din Monib0Hanan Hassan Sabry1Mohamed Saber Hussein2Asmaa Adel El-fallah3Rehab Mohammed Salem4Department of Dermatology and Andrology, Faculty of Medicine, Benha UniversityDepartment of Dermatology and Andrology, Faculty of Medicine, Benha UniversityDepartment of Dermatology and Andrology, Faculty of Medicine, Benha UniversityDepartment of Clinical and Chemical Pathology, Faculty of Medicine, Benha UniversityDepartment of Dermatology and Andrology, Faculty of Medicine, Benha UniversityBackground Factors contributing to the pathogenesis of vitiligo and factors affecting its response to treatment are still a major area of debate. Aim of the work The study aimed to assess the serum levels of tyrosinase and Micro-RNAs (miRNAs) gene polymorphism in a sample of Egyptian vitiligo patients, and to determine factors affecting the response of vitiligo to treatment. Subjects and methods This prospective case-control interventional study included 212 non-segmental vitiligo patients and 96 control subjects. Before treatment, vitiligo was evaluated using Vitiligo Area Severity Index. Detection of miRNA 196a-2 polymorphism was done using PCR-REELP and serum tyrosinase was measured using ELISA. After treatment, patients were reevaluated clinically and serum tyrosinase levels were re-measured. Results The tyrosinase levels were significantly elevated in patients. The TT genotype was the most prevalent one in the patients. The percentage of improvement showed a significant positive correlation with patients' ages and age of the disease onset and a negative correlation with disease duration, baseline VASI scores and serum tyrosinase levels. Conclusion MiRNA 196a-2 C/T (11614913) gene polymorphism and the elevated serum tyrosinase levels might be related to the pathogenesis of vitiligo and may affect its therapeutic response.http://dx.doi.org/10.1080/09546634.2020.1810202mirna 196a2c/tresponsetyrosinasevitiligo |
| spellingShingle | Khaled Mohey El-Din Monib Hanan Hassan Sabry Mohamed Saber Hussein Asmaa Adel El-fallah Rehab Mohammed Salem Factors affecting vitiligo response to treatment: do MiRNA 196a2C/T gene polymorphism and serum tyrosinase levels have any role? Journal of Dermatological Treatment mirna 196a2c/t response tyrosinase vitiligo |
| title | Factors affecting vitiligo response to treatment: do MiRNA 196a2C/T gene polymorphism and serum tyrosinase levels have any role? |
| title_full | Factors affecting vitiligo response to treatment: do MiRNA 196a2C/T gene polymorphism and serum tyrosinase levels have any role? |
| title_fullStr | Factors affecting vitiligo response to treatment: do MiRNA 196a2C/T gene polymorphism and serum tyrosinase levels have any role? |
| title_full_unstemmed | Factors affecting vitiligo response to treatment: do MiRNA 196a2C/T gene polymorphism and serum tyrosinase levels have any role? |
| title_short | Factors affecting vitiligo response to treatment: do MiRNA 196a2C/T gene polymorphism and serum tyrosinase levels have any role? |
| title_sort | factors affecting vitiligo response to treatment do mirna 196a2c t gene polymorphism and serum tyrosinase levels have any role |
| topic | mirna 196a2c/t response tyrosinase vitiligo |
| url | http://dx.doi.org/10.1080/09546634.2020.1810202 |
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