High-level expression and purification of Plutella xylostella acetylcholinesterase in Pichia pastoris and its potential application

High-level expression and purification of Plutella xylostella acetylcholinesterase in Pichia pastoris and its potential application

The acetylcholinesterase 2 (AChE2) cloned from Plutella xylostella was first successfully expressed in methylotrophic yeast Pichia pastoris GS115. One transformant with high-level expression of the recombinant AChE (rAChE, 23.2 U mL−1 in supernatant) was selected by plating on increasing concentrati...

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Bibliographic Details
Main Authors:	Niu LIU, Jun CHE, Duo LAI, Jin-jun WEN, Han-hong XU
Format:	Article
Language:	English
Published:	Elsevier 2017-06-01
Series:	Journal of Integrative Agriculture
Subjects:	Plutella xylostella acetylcholinesterase Pichia pastoris high-level expression
Online Access:	http://www.sciencedirect.com/science/article/pii/S2095311916615083

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