Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta)
In vitro culture of spermatogonial stem cells (SSCs) has generally been performed using two-dimensional (2D) culture systems; however, such cultures have not led to the development of complete spermatogenesis. It seems that 2D systems do not replicate optimal conditions of the seminiferous tubules (...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Asian Journal of Andrology |
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| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2015;volume=17;issue=6;spage=972;epage=980;aulast=Huleihel |
| _version_ | 1818534904568217600 |
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| author | Mahmoud Huleihel Seyedmehdi Nourashrafeddin Tony M Plant |
| author_facet | Mahmoud Huleihel Seyedmehdi Nourashrafeddin Tony M Plant |
| author_sort | Mahmoud Huleihel |
| collection | DOAJ |
| description | In vitro culture of spermatogonial stem cells (SSCs) has generally been performed using two-dimensional (2D) culture systems; however, such cultures have not led to the development of complete spermatogenesis. It seems that 2D systems do not replicate optimal conditions of the seminiferous tubules (including those generated by the SSC niche) and necessary for spermatogenesis. Recently, one of our laboratories has been able to induce proliferation and differentiation of mouse testicular germ cells to meiotic and postmeiotic stages including generation of sperm in a 3D soft agar culture system (SACS) and a 3D methylcellulose culture system (MCS). It was suggested that SACS and MCS form a special 3D microenvironment that mimics germ cell niche formation in the seminiferous tubules, and thus permits mouse spermatogenesis in vitro. In this review, we (1) provide a brief overview of the differences in spermatogenesis in rodents and primates, (2) summarize data related to attempts to generate sperm in vitro, (3) report for the first time formation of colonies/clusters of cells and differentiation of meiotic (expression of CREM-1) and postmeiotic (expression of acrosin) germ cells from undifferentiated spermatogonia isolated from the testis of prepubertal rhesus monkeys and cultured in SACS and MCS, and (4) indicate research needed to optimize 3D systems for in vitroprimate spermatogenesis and for possible future application to man. |
| first_indexed | 2024-12-11T18:17:41Z |
| format | Article |
| id | doaj.art-ffabff4c88954e9bbd5fddc936699017 |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-12-11T18:17:41Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-ffabff4c88954e9bbd5fddc9366990172022-12-22T00:55:23ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622015-01-0117697298010.4103/1008-682X.154994Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta)Mahmoud HuleihelSeyedmehdi NourashrafeddinTony M PlantIn vitro culture of spermatogonial stem cells (SSCs) has generally been performed using two-dimensional (2D) culture systems; however, such cultures have not led to the development of complete spermatogenesis. It seems that 2D systems do not replicate optimal conditions of the seminiferous tubules (including those generated by the SSC niche) and necessary for spermatogenesis. Recently, one of our laboratories has been able to induce proliferation and differentiation of mouse testicular germ cells to meiotic and postmeiotic stages including generation of sperm in a 3D soft agar culture system (SACS) and a 3D methylcellulose culture system (MCS). It was suggested that SACS and MCS form a special 3D microenvironment that mimics germ cell niche formation in the seminiferous tubules, and thus permits mouse spermatogenesis in vitro. In this review, we (1) provide a brief overview of the differences in spermatogenesis in rodents and primates, (2) summarize data related to attempts to generate sperm in vitro, (3) report for the first time formation of colonies/clusters of cells and differentiation of meiotic (expression of CREM-1) and postmeiotic (expression of acrosin) germ cells from undifferentiated spermatogonia isolated from the testis of prepubertal rhesus monkeys and cultured in SACS and MCS, and (4) indicate research needed to optimize 3D systems for in vitroprimate spermatogenesis and for possible future application to man.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2015;volume=17;issue=6;spage=972;epage=980;aulast=Huleihelin vitro spermatogenesis; methylcellulose culture system; monkey; primates; soft agar culture system; three-dimensional culture system |
| spellingShingle | Mahmoud Huleihel Seyedmehdi Nourashrafeddin Tony M Plant Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta) Asian Journal of Andrology in vitro spermatogenesis; methylcellulose culture system; monkey; primates; soft agar culture system; three-dimensional culture system |
| title | Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta) |
| title_full | Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta) |
| title_fullStr | Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta) |
| title_full_unstemmed | Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta) |
| title_short | Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta) |
| title_sort | application of three dimensional culture systems to study mammalian spermatogenesis with an emphasis on the rhesus monkey macaca mulatta |
| topic | in vitro spermatogenesis; methylcellulose culture system; monkey; primates; soft agar culture system; three-dimensional culture system |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2015;volume=17;issue=6;spage=972;epage=980;aulast=Huleihel |
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