| Summary: | Aflatoxin contamination of maize is a leading threat to health in Guatemala. This contamination is the result of infection from <i>Aspergillus flavus</i> and has been effectively reduced in other countries through application of nonaflatoxigenic, indigenous strains of <i>A. flavus</i>. We collected 82 maize samples from throughout Guatemala in two years and isolated 272 <i>A. flavus</i> from these samples, including 126 unique genotypes. We provide here a phenotypic and simple sequence repeat (SSR)-based genotypic description of these isolates, as well as an analysis of the diversity of this population. High levels of genetic diversity were observed with the nonaflatoxigenic isolates in this study, but this information contributes to the development of indigenous aflatoxin biocontrol products.
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