Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations
Abstract Background The hemibiotrophic pathogens Moniliophthora perniciosa (witches’ broom disease) and Moniliophthora roreri (frosty pod rot disease) are among the most important pathogens of cacao. Moniliophthora perniciosa has a broad host range and infects a variety of meristematic tissues in ca...
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BMC
2018-07-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-018-4875-7 |
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| author | Ceslaine Santos Barbosa Rute R. da Fonseca Thiago Mafra Batista Mariana Araújo Barreto Caio Suzart Argolo Mariana Rocha de Carvalho Daniel Oliveira Jordão do Amaral Edson Mário de Andrade Silva Enrique Arévalo-Gardini Karina Solis Hidalgo Glória Regina Franco Carlos Priminho Pirovani Fabienne Micheli Karina Peres Gramacho |
| author_facet | Ceslaine Santos Barbosa Rute R. da Fonseca Thiago Mafra Batista Mariana Araújo Barreto Caio Suzart Argolo Mariana Rocha de Carvalho Daniel Oliveira Jordão do Amaral Edson Mário de Andrade Silva Enrique Arévalo-Gardini Karina Solis Hidalgo Glória Regina Franco Carlos Priminho Pirovani Fabienne Micheli Karina Peres Gramacho |
| author_sort | Ceslaine Santos Barbosa |
| collection | DOAJ |
| description | Abstract Background The hemibiotrophic pathogens Moniliophthora perniciosa (witches’ broom disease) and Moniliophthora roreri (frosty pod rot disease) are among the most important pathogens of cacao. Moniliophthora perniciosa has a broad host range and infects a variety of meristematic tissues in cacao plants, whereas M. roreri infects only pods of Theobroma and Herrania genera. Comparative pathogenomics of these fungi is essential to understand Moniliophthora infection strategies, therefore the detection and in silico functional characterization of effector candidates are important steps to gain insight on their pathogenicity. Results Candidate secreted effector proteins repertoire were predicted using the genomes of five representative isolates of M. perniciosa subpopulations (three from cacao and two from solanaceous hosts), and one representative isolate of M. roreri from Peru. Many putative effectors candidates were identified in M. perniciosa: 157 and 134 in cacao isolates from Bahia, Brazil; 109 in cacao isolate from Ecuador, 92 and 80 in wild solanaceous isolates from Minas Gerais (Lobeira) and Bahia (Caiçara), Brazil; respectively. Moniliophthora roreri showed the highest number of effector candidates, a total of 243. A set of eight core effectors were shared among all Moniliophthora isolates, while others were shared either between the wild solanaceous isolates or among cacao isolates. Mostly, candidate effectors of M. perniciosa were shared among the isolates, whereas in M. roreri nearly 50% were exclusive to the specie. In addition, a large number of cell wall-degrading enzymes characteristic of hemibiotrophic fungi were found. From these, we highlighted the proteins involved in cell wall modification, an enzymatic arsenal that allows the plant pathogens to inhabit environments with oxidative stress, which promotes degradation of plant compounds and facilitates infection. Conclusions The present work reports six genomes and provides a database of the putative effectorome of Moniliophthora, a first step towards the understanding of the functional basis of fungal pathogenicity. |
| first_indexed | 2024-12-23T10:02:27Z |
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| language | English |
| last_indexed | 2024-12-23T10:02:27Z |
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| series | BMC Genomics |
| spelling | doaj.art-ffae58b398df444fac9341efbf70c0e62022-12-21T17:51:10ZengBMCBMC Genomics1471-21642018-07-0119111210.1186/s12864-018-4875-7Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulationsCeslaine Santos Barbosa0Rute R. da Fonseca1Thiago Mafra Batista2Mariana Araújo Barreto3Caio Suzart Argolo4Mariana Rocha de Carvalho5Daniel Oliveira Jordão do Amaral6Edson Mário de Andrade Silva7Enrique Arévalo-Gardini8Karina Solis Hidalgo9Glória Regina Franco10Carlos Priminho Pirovani11Fabienne Micheli12Karina Peres Gramacho13Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Universidade Estadual de Santa Cruz (UESC)The Bioinformatics Centre, Department of Biology, University of CopenhagenDepartamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais/Belo HorizonteDepartamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Universidade Estadual de Santa Cruz (UESC)Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Universidade Estadual de Santa Cruz (UESC)Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Universidade Estadual de Santa Cruz (UESC)Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Universidade Estadual de Santa Cruz (UESC)Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Universidade Estadual de Santa Cruz (UESC)Instituto de Cultivos Tropicales –ICTInstituto Nacional de Investigaciones Agropecuarias – INIAP, Departamento de Protección VegetalDepartamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais/Belo HorizonteDepartamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Universidade Estadual de Santa Cruz (UESC)Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Universidade Estadual de Santa Cruz (UESC)Departamento de Ciências Biológicas (DCB), Centro de Biotecnologia e Genética (CBG), Universidade Estadual de Santa Cruz (UESC)Abstract Background The hemibiotrophic pathogens Moniliophthora perniciosa (witches’ broom disease) and Moniliophthora roreri (frosty pod rot disease) are among the most important pathogens of cacao. Moniliophthora perniciosa has a broad host range and infects a variety of meristematic tissues in cacao plants, whereas M. roreri infects only pods of Theobroma and Herrania genera. Comparative pathogenomics of these fungi is essential to understand Moniliophthora infection strategies, therefore the detection and in silico functional characterization of effector candidates are important steps to gain insight on their pathogenicity. Results Candidate secreted effector proteins repertoire were predicted using the genomes of five representative isolates of M. perniciosa subpopulations (three from cacao and two from solanaceous hosts), and one representative isolate of M. roreri from Peru. Many putative effectors candidates were identified in M. perniciosa: 157 and 134 in cacao isolates from Bahia, Brazil; 109 in cacao isolate from Ecuador, 92 and 80 in wild solanaceous isolates from Minas Gerais (Lobeira) and Bahia (Caiçara), Brazil; respectively. Moniliophthora roreri showed the highest number of effector candidates, a total of 243. A set of eight core effectors were shared among all Moniliophthora isolates, while others were shared either between the wild solanaceous isolates or among cacao isolates. Mostly, candidate effectors of M. perniciosa were shared among the isolates, whereas in M. roreri nearly 50% were exclusive to the specie. In addition, a large number of cell wall-degrading enzymes characteristic of hemibiotrophic fungi were found. From these, we highlighted the proteins involved in cell wall modification, an enzymatic arsenal that allows the plant pathogens to inhabit environments with oxidative stress, which promotes degradation of plant compounds and facilitates infection. Conclusions The present work reports six genomes and provides a database of the putative effectorome of Moniliophthora, a first step towards the understanding of the functional basis of fungal pathogenicity.http://link.springer.com/article/10.1186/s12864-018-4875-7Theobroma cacaoWitches’ broomFrosty pod rotPathogenicity factorsPlant pathogens |
| spellingShingle | Ceslaine Santos Barbosa Rute R. da Fonseca Thiago Mafra Batista Mariana Araújo Barreto Caio Suzart Argolo Mariana Rocha de Carvalho Daniel Oliveira Jordão do Amaral Edson Mário de Andrade Silva Enrique Arévalo-Gardini Karina Solis Hidalgo Glória Regina Franco Carlos Priminho Pirovani Fabienne Micheli Karina Peres Gramacho Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations BMC Genomics Theobroma cacao Witches’ broom Frosty pod rot Pathogenicity factors Plant pathogens |
| title | Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations |
| title_full | Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations |
| title_fullStr | Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations |
| title_full_unstemmed | Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations |
| title_short | Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations |
| title_sort | genome sequence and effectorome of moniliophthora perniciosa and moniliophthora roreri subpopulations |
| topic | Theobroma cacao Witches’ broom Frosty pod rot Pathogenicity factors Plant pathogens |
| url | http://link.springer.com/article/10.1186/s12864-018-4875-7 |
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