The Potential of Colonic Tumor Tissue <i>Fusobacterium nucleatum</i> to Predict Staging and Its Interplay with Oral Abundance in Colon Cancer Patients
Background. Intestinal microbiota dysbiosis may enhance the carcinogenicity of colon cancer (CC) by the proliferation and differentiation of epithelial cells. Oral <i>Fusobacterium nucleatum</i> (<i>Fn</i>) and <i>Porphyromonas gingivalis</i> (<i>Pg</i>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/5/1032 |
| _version_ | 1797416413334339584 |
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| author | Pamela Pignatelli Lorena Iezzi Martina Pennese Paolo Raimondi Anna Cichella Danilo Bondi Rossella Grande Roberto Cotellese Nicola Di Bartolomeo Paolo Innocenti Adriano Piattelli Maria Cristina Curia |
| author_facet | Pamela Pignatelli Lorena Iezzi Martina Pennese Paolo Raimondi Anna Cichella Danilo Bondi Rossella Grande Roberto Cotellese Nicola Di Bartolomeo Paolo Innocenti Adriano Piattelli Maria Cristina Curia |
| author_sort | Pamela Pignatelli |
| collection | DOAJ |
| description | Background. Intestinal microbiota dysbiosis may enhance the carcinogenicity of colon cancer (CC) by the proliferation and differentiation of epithelial cells. Oral <i>Fusobacterium nucleatum</i> (<i>Fn</i>) and <i>Porphyromonas gingivalis</i> (<i>Pg</i>) have the ability to invade the gut epithelium, promoting tumor progression. The aim of the study was to assess whether the abundance of these odontopathogenic bacteria was associated with colon cancer. We also investigated how lifestyle factors could influence the oral <i>Fn</i> and <i>Pg</i> abundance and CC. Methods. Thirty-six CC patients were included in the study to assess the <i>Pg</i> and <i>Fn</i> oral and colon tissue abundance by qPCR. Oral health data, food habits and lifestyles were also recorded. Results. Patients had a greater quantity of <i>Fn</i> in the oral cavity than matched CC and adjacent non-neoplastic mucosa (adj t) tissues (<i>p</i> = 0.004 and <i>p</i> < 0.001). Instead, <i>Pg</i> was not significantly detected in colonic tissues. There was an association between the <i>Fn</i> quantity in the oral and CC tissue and a statistically significant relation between the <i>Fn</i> abundance in adenocarcinoma (ADK) and staging (<i>p</i> = 0.016). The statistical analysis revealed a tendency towards a greater <i>Fn</i> quantity in CC (<i>p</i> = 0.073, η<sup>2</sup><i><sub>p</sub></i> = 0.12) for high-meat consumers. Conclusion. In our study, <i>Pg</i> was absent in colon tissues but was correlated with the oral inflammation gingival and plaque indices. For the first time, there was evidence that the <i>Fn</i> oral concentration can influence colon tissue concentrations and predict CC prognosis. |
| first_indexed | 2024-03-09T06:03:52Z |
| format | Article |
| id | doaj.art-ffae8dfed9fb4c15b0f181c960ecaa73 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T06:03:52Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-ffae8dfed9fb4c15b0f181c960ecaa732023-12-03T12:07:21ZengMDPI AGCancers2072-66942021-03-01135103210.3390/cancers13051032The Potential of Colonic Tumor Tissue <i>Fusobacterium nucleatum</i> to Predict Staging and Its Interplay with Oral Abundance in Colon Cancer PatientsPamela Pignatelli0Lorena Iezzi1Martina Pennese2Paolo Raimondi3Anna Cichella4Danilo Bondi5Rossella Grande6Roberto Cotellese7Nicola Di Bartolomeo8Paolo Innocenti9Adriano Piattelli10Maria Cristina Curia11Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini, 66100 Chieti, ItalyDepartment of General Surgery, Private Hospital “Villa Serena”, Città Sant’Angelo, 65013 Pescara, ItalyDepartment of General Surgery, Private Hospital “Villa Serena”, Città Sant’Angelo, 65013 Pescara, ItalyDepartment of Neuroscience, Imaging and Clinical Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini, 66100 Chieti, ItalyDepartment of Pharmacy, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini, 66100 Chieti, ItalyDepartment of General Surgery, Private Hospital “Villa Serena”, Città Sant’Angelo, 65013 Pescara, ItalyDepartment of General Surgery, Private Hospital “Villa Serena”, Città Sant’Angelo, 65013 Pescara, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini, 66100 Chieti, ItalyBackground. Intestinal microbiota dysbiosis may enhance the carcinogenicity of colon cancer (CC) by the proliferation and differentiation of epithelial cells. Oral <i>Fusobacterium nucleatum</i> (<i>Fn</i>) and <i>Porphyromonas gingivalis</i> (<i>Pg</i>) have the ability to invade the gut epithelium, promoting tumor progression. The aim of the study was to assess whether the abundance of these odontopathogenic bacteria was associated with colon cancer. We also investigated how lifestyle factors could influence the oral <i>Fn</i> and <i>Pg</i> abundance and CC. Methods. Thirty-six CC patients were included in the study to assess the <i>Pg</i> and <i>Fn</i> oral and colon tissue abundance by qPCR. Oral health data, food habits and lifestyles were also recorded. Results. Patients had a greater quantity of <i>Fn</i> in the oral cavity than matched CC and adjacent non-neoplastic mucosa (adj t) tissues (<i>p</i> = 0.004 and <i>p</i> < 0.001). Instead, <i>Pg</i> was not significantly detected in colonic tissues. There was an association between the <i>Fn</i> quantity in the oral and CC tissue and a statistically significant relation between the <i>Fn</i> abundance in adenocarcinoma (ADK) and staging (<i>p</i> = 0.016). The statistical analysis revealed a tendency towards a greater <i>Fn</i> quantity in CC (<i>p</i> = 0.073, η<sup>2</sup><i><sub>p</sub></i> = 0.12) for high-meat consumers. Conclusion. In our study, <i>Pg</i> was absent in colon tissues but was correlated with the oral inflammation gingival and plaque indices. For the first time, there was evidence that the <i>Fn</i> oral concentration can influence colon tissue concentrations and predict CC prognosis.https://www.mdpi.com/2072-6694/13/5/1032oral microbiota<i>Fusobacterium nucleatum</i>colon cancerabundancedysbiosisdiet |
| spellingShingle | Pamela Pignatelli Lorena Iezzi Martina Pennese Paolo Raimondi Anna Cichella Danilo Bondi Rossella Grande Roberto Cotellese Nicola Di Bartolomeo Paolo Innocenti Adriano Piattelli Maria Cristina Curia The Potential of Colonic Tumor Tissue <i>Fusobacterium nucleatum</i> to Predict Staging and Its Interplay with Oral Abundance in Colon Cancer Patients Cancers oral microbiota <i>Fusobacterium nucleatum</i> colon cancer abundance dysbiosis diet |
| title | The Potential of Colonic Tumor Tissue <i>Fusobacterium nucleatum</i> to Predict Staging and Its Interplay with Oral Abundance in Colon Cancer Patients |
| title_full | The Potential of Colonic Tumor Tissue <i>Fusobacterium nucleatum</i> to Predict Staging and Its Interplay with Oral Abundance in Colon Cancer Patients |
| title_fullStr | The Potential of Colonic Tumor Tissue <i>Fusobacterium nucleatum</i> to Predict Staging and Its Interplay with Oral Abundance in Colon Cancer Patients |
| title_full_unstemmed | The Potential of Colonic Tumor Tissue <i>Fusobacterium nucleatum</i> to Predict Staging and Its Interplay with Oral Abundance in Colon Cancer Patients |
| title_short | The Potential of Colonic Tumor Tissue <i>Fusobacterium nucleatum</i> to Predict Staging and Its Interplay with Oral Abundance in Colon Cancer Patients |
| title_sort | potential of colonic tumor tissue i fusobacterium nucleatum i to predict staging and its interplay with oral abundance in colon cancer patients |
| topic | oral microbiota <i>Fusobacterium nucleatum</i> colon cancer abundance dysbiosis diet |
| url | https://www.mdpi.com/2072-6694/13/5/1032 |
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