The Impact of the Inoculation of Phosphate-Solubilizing Bacteria <i>Pantoea agglomerans</i> on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil
The bacterial genus <i>Pantoea</i> has been widely evaluated as promising bacteria to increase phosphorus (P) availability in soil. The aim of this study was to characterize the phosphate solubilizing (PS) activity of a <i>Pantoea agglomerans</i> strain and to evaluate the im...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/8/1661 |
| _version_ | 1797522887260766208 |
|---|---|
| author | Ilhem Saadouli Amor Mosbah Raoudha Ferjani Panagiota Stathopoulou Ioannis Galiatsatos Elias Asimakis Ramona Marasco Daniele Daffonchio George Tsiamis Hadda-Imene Ouzari |
| author_facet | Ilhem Saadouli Amor Mosbah Raoudha Ferjani Panagiota Stathopoulou Ioannis Galiatsatos Elias Asimakis Ramona Marasco Daniele Daffonchio George Tsiamis Hadda-Imene Ouzari |
| author_sort | Ilhem Saadouli |
| collection | DOAJ |
| description | The bacterial genus <i>Pantoea</i> has been widely evaluated as promising bacteria to increase phosphorus (P) availability in soil. The aim of this study was to characterize the phosphate solubilizing (PS) activity of a <i>Pantoea agglomerans</i> strain and to evaluate the impact of its application in a semi-arid soil on phosphate availability and structure of the bacterial communities as a whole. An incubation experiment under close-to-natural soil environmental conditions was conducted for 15 days at 30 °C. High-throughput sequencing of the bacterial 16S rRNA gene was used to characterize and to compare the bacterial community structure of <i>P. agglomerans</i>-inoculated soil with non-inoculated control. Furthermore, a qPCR-based method was developed for detection and quantification of the functional genes related to the expression of mineral phosphate solubilization (MPS) phenotype in <i>P. agglomerans</i>. The results showed that in vitro solubilization of Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> by <i>P. agglomerans</i> strain was very efficient (980 mg/L), and it was associated with a drop in pH due to the secretion of gluconic acid; these changes were concomitant with the detection of <i>gdh</i> and <i>pqqC</i> genes. Moreover, <i>P. agglomerans</i> inoculum application significantly increased the content of available P in semi-arid soil by 69%. Metagenomic analyses showed that <i>P. agglomerans</i> treatment modified the overall edaphic bacterial community, significantly impacting its structure and composition. In particular, during <i>P. agglomerans</i> inoculation the relative abundance of bacteria belonging to Firmicutes (mainly <i>Bacilli</i> class) significantly increased, whereas the abundance of Actinobacteria together with Acidobacteria and Chloroflexi phyla decreased. Furthermore, genera known for their phosphate solubilizing activity, such as <i>Aneurinibacillus</i>, <i>Lysinibacillus</i>, <i>Enterococcus</i>, and <i>Pontibacter,</i> were exclusively detected in <i>P. agglomerans</i>-treated soil. Pearson’s correlation analysis revealed that changes in soil bacterial community composition were closely affected by soil characteristics, such as pH and available P. This study explores the effect of the inoculation of <i>P. agglomerans</i> on the bacterial community structure of a semi-arid soil. The effectiveness in improving the phosphate availability and modification in soil bacterial community suggested that <i>P. agglomerans</i> represent a promising environmental-friendly biofertilizer in arid and semi-arid ecosystems. |
| first_indexed | 2024-03-10T08:34:45Z |
| format | Article |
| id | doaj.art-ffb015d27f834009822d7e957519dc43 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T08:34:45Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ffb015d27f834009822d7e957519dc432023-11-22T08:47:00ZengMDPI AGMicroorganisms2076-26072021-08-0198166110.3390/microorganisms9081661The Impact of the Inoculation of Phosphate-Solubilizing Bacteria <i>Pantoea agglomerans</i> on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid SoilIlhem Saadouli0Amor Mosbah1Raoudha Ferjani2Panagiota Stathopoulou3Ioannis Galiatsatos4Elias Asimakis5Ramona Marasco6Daniele Daffonchio7George Tsiamis8Hadda-Imene Ouzari9Laboratoire de Microorganismes et Biomolécules Actives (LR03ES03), Facultédes Sciences de Tunis, Université Tunis El Manar, 2092 Tunis, TunisiaHigher Institute for Biotechnology (ISBST), LR Biotechnology and Bio-Geo Resources Valorization, University of Manouba, BVBGR-LR11ES31, Biotechpole Sidi Thabet, 2020 Ariana, TunisiaLaboratoire de Microorganismes et Biomolécules Actives (LR03ES03), Facultédes Sciences de Tunis, Université Tunis El Manar, 2092 Tunis, TunisiaLaboratory of Systems Microbiology and Applied Genomics, Department of Environmental Engineering, University of Patras, 2 Seferi St., 30100 Agrinio, GreeceLaboratory of Systems Microbiology and Applied Genomics, Department of Environmental Engineering, University of Patras, 2 Seferi St., 30100 Agrinio, GreeceLaboratory of Systems Microbiology and Applied Genomics, Department of Environmental Engineering, University of Patras, 2 Seferi St., 30100 Agrinio, GreeceRed Sea Research Center (RSRC), Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi ArabiaRed Sea Research Center (RSRC), Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi ArabiaLaboratory of Systems Microbiology and Applied Genomics, Department of Environmental Engineering, University of Patras, 2 Seferi St., 30100 Agrinio, GreeceLaboratoire de Microorganismes et Biomolécules Actives (LR03ES03), Facultédes Sciences de Tunis, Université Tunis El Manar, 2092 Tunis, TunisiaThe bacterial genus <i>Pantoea</i> has been widely evaluated as promising bacteria to increase phosphorus (P) availability in soil. The aim of this study was to characterize the phosphate solubilizing (PS) activity of a <i>Pantoea agglomerans</i> strain and to evaluate the impact of its application in a semi-arid soil on phosphate availability and structure of the bacterial communities as a whole. An incubation experiment under close-to-natural soil environmental conditions was conducted for 15 days at 30 °C. High-throughput sequencing of the bacterial 16S rRNA gene was used to characterize and to compare the bacterial community structure of <i>P. agglomerans</i>-inoculated soil with non-inoculated control. Furthermore, a qPCR-based method was developed for detection and quantification of the functional genes related to the expression of mineral phosphate solubilization (MPS) phenotype in <i>P. agglomerans</i>. The results showed that in vitro solubilization of Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> by <i>P. agglomerans</i> strain was very efficient (980 mg/L), and it was associated with a drop in pH due to the secretion of gluconic acid; these changes were concomitant with the detection of <i>gdh</i> and <i>pqqC</i> genes. Moreover, <i>P. agglomerans</i> inoculum application significantly increased the content of available P in semi-arid soil by 69%. Metagenomic analyses showed that <i>P. agglomerans</i> treatment modified the overall edaphic bacterial community, significantly impacting its structure and composition. In particular, during <i>P. agglomerans</i> inoculation the relative abundance of bacteria belonging to Firmicutes (mainly <i>Bacilli</i> class) significantly increased, whereas the abundance of Actinobacteria together with Acidobacteria and Chloroflexi phyla decreased. Furthermore, genera known for their phosphate solubilizing activity, such as <i>Aneurinibacillus</i>, <i>Lysinibacillus</i>, <i>Enterococcus</i>, and <i>Pontibacter,</i> were exclusively detected in <i>P. agglomerans</i>-treated soil. Pearson’s correlation analysis revealed that changes in soil bacterial community composition were closely affected by soil characteristics, such as pH and available P. This study explores the effect of the inoculation of <i>P. agglomerans</i> on the bacterial community structure of a semi-arid soil. The effectiveness in improving the phosphate availability and modification in soil bacterial community suggested that <i>P. agglomerans</i> represent a promising environmental-friendly biofertilizer in arid and semi-arid ecosystems.https://www.mdpi.com/2076-2607/9/8/1661available Pmicrobial inoculantssoil bacterial community compositionsoil bacterial community structurehigh-throughput sequencing |
| spellingShingle | Ilhem Saadouli Amor Mosbah Raoudha Ferjani Panagiota Stathopoulou Ioannis Galiatsatos Elias Asimakis Ramona Marasco Daniele Daffonchio George Tsiamis Hadda-Imene Ouzari The Impact of the Inoculation of Phosphate-Solubilizing Bacteria <i>Pantoea agglomerans</i> on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil Microorganisms available P microbial inoculants soil bacterial community composition soil bacterial community structure high-throughput sequencing |
| title | The Impact of the Inoculation of Phosphate-Solubilizing Bacteria <i>Pantoea agglomerans</i> on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil |
| title_full | The Impact of the Inoculation of Phosphate-Solubilizing Bacteria <i>Pantoea agglomerans</i> on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil |
| title_fullStr | The Impact of the Inoculation of Phosphate-Solubilizing Bacteria <i>Pantoea agglomerans</i> on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil |
| title_full_unstemmed | The Impact of the Inoculation of Phosphate-Solubilizing Bacteria <i>Pantoea agglomerans</i> on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil |
| title_short | The Impact of the Inoculation of Phosphate-Solubilizing Bacteria <i>Pantoea agglomerans</i> on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil |
| title_sort | impact of the inoculation of phosphate solubilizing bacteria i pantoea agglomerans i on phosphorus availability and bacterial community dynamics of a semi arid soil |
| topic | available P microbial inoculants soil bacterial community composition soil bacterial community structure high-throughput sequencing |
| url | https://www.mdpi.com/2076-2607/9/8/1661 |
| work_keys_str_mv | AT ilhemsaadouli theimpactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT amormosbah theimpactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT raoudhaferjani theimpactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT panagiotastathopoulou theimpactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT ioannisgaliatsatos theimpactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT eliasasimakis theimpactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT ramonamarasco theimpactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT danieledaffonchio theimpactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT georgetsiamis theimpactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT haddaimeneouzari theimpactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT ilhemsaadouli impactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT amormosbah impactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT raoudhaferjani impactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT panagiotastathopoulou impactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT ioannisgaliatsatos impactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT eliasasimakis impactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT ramonamarasco impactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT danieledaffonchio impactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT georgetsiamis impactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil AT haddaimeneouzari impactoftheinoculationofphosphatesolubilizingbacteriaipantoeaagglomeransionphosphorusavailabilityandbacterialcommunitydynamicsofasemiaridsoil |