The Role of <i>ptsH</i> in Stress Adaptation and Virulence in <i>Cronobacter sakazakii</i> BAA-894
<i>Cronobacter sakazakii</i>, an emerging foodborne pathogen that was isolated primarily from powdered infant formula, poses an important issue in food safety due to its high stress tolerance and pathogenicity. The Hpr (encoded by <i>ptsH</i> gene) has been shown to regulate...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/17/2680 |
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| author | Yi Sun Jiahui Li Yanpeng Yang Gaoji Yang Yiqi Shi Shuo Wang Muxue Wang Xiaodong Xia |
| author_facet | Yi Sun Jiahui Li Yanpeng Yang Gaoji Yang Yiqi Shi Shuo Wang Muxue Wang Xiaodong Xia |
| author_sort | Yi Sun |
| collection | DOAJ |
| description | <i>Cronobacter sakazakii</i>, an emerging foodborne pathogen that was isolated primarily from powdered infant formula, poses an important issue in food safety due to its high stress tolerance and pathogenicity. The Hpr (encoded by <i>ptsH</i> gene) has been shown to regulate carbon metabolism as well as stress response and virulence. However, the functional properties of <i>ptsH</i> in <i>C. sakzakii</i> have not been investigated. In this study, we clarified the role of <i>ptsH</i> in the <i>C. sakzakii</i> stress response and virulence, and explored its possible regulatory mechanism by RNA-seq. Compared with wild-type, the Δ<i>ptsH</i> mutant showed a slower growth rate in the log phase but no difference in the stationary phase. Moreover, the resistance to heat stress (65 °C, 55 °C), simulated gastric fluid (pH = 2.5), biofilm formation and adhesion to HT-29 cells of Δ<i>ptsH</i> mutant were significantly decreased, whereas the oxidative resistance (1, 5, 10 mM H<sub>2</sub>O<sub>2</sub>), osmotic resistance (10%, 15%, 20% NaCl), and superoxide dismutase activity were enhanced. Finally, RNA-seq analysis revealed the sulfur metabolism pathway is significantly upregulated in the Δ<i>ptsH</i> mutant, but the bacterial secretion system pathway is dramatically downregulated. The qRT-PCR assay further demonstrated that the Δ<i>ptsH</i> mutant has elevated levels of genes that are related to oxidative and osmotic stress (<i>sodA</i>, <i>rpoS</i>, <i>cpxA</i>/<i>R</i>, <i>osmY</i>). This study provides a great understanding of the role of <i>ptsH</i> in diverse stress responses and virulence in <i>C. sakazakii</i>, and it contributes to our understanding of the genetic determinant of stress resistance and pathogenicity of this important foodborne pathogen. |
| first_indexed | 2024-03-10T01:49:39Z |
| format | Article |
| id | doaj.art-ffb3034f7cb74c7faad71c6764c177c8 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T01:49:39Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-ffb3034f7cb74c7faad71c6764c177c82023-11-23T13:09:30ZengMDPI AGFoods2304-81582022-09-011117268010.3390/foods11172680The Role of <i>ptsH</i> in Stress Adaptation and Virulence in <i>Cronobacter sakazakii</i> BAA-894Yi Sun0Jiahui Li1Yanpeng Yang2Gaoji Yang3Yiqi Shi4Shuo Wang5Muxue Wang6Xiaodong Xia7College of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianyang 712100, China<i>Cronobacter sakazakii</i>, an emerging foodborne pathogen that was isolated primarily from powdered infant formula, poses an important issue in food safety due to its high stress tolerance and pathogenicity. The Hpr (encoded by <i>ptsH</i> gene) has been shown to regulate carbon metabolism as well as stress response and virulence. However, the functional properties of <i>ptsH</i> in <i>C. sakzakii</i> have not been investigated. In this study, we clarified the role of <i>ptsH</i> in the <i>C. sakzakii</i> stress response and virulence, and explored its possible regulatory mechanism by RNA-seq. Compared with wild-type, the Δ<i>ptsH</i> mutant showed a slower growth rate in the log phase but no difference in the stationary phase. Moreover, the resistance to heat stress (65 °C, 55 °C), simulated gastric fluid (pH = 2.5), biofilm formation and adhesion to HT-29 cells of Δ<i>ptsH</i> mutant were significantly decreased, whereas the oxidative resistance (1, 5, 10 mM H<sub>2</sub>O<sub>2</sub>), osmotic resistance (10%, 15%, 20% NaCl), and superoxide dismutase activity were enhanced. Finally, RNA-seq analysis revealed the sulfur metabolism pathway is significantly upregulated in the Δ<i>ptsH</i> mutant, but the bacterial secretion system pathway is dramatically downregulated. The qRT-PCR assay further demonstrated that the Δ<i>ptsH</i> mutant has elevated levels of genes that are related to oxidative and osmotic stress (<i>sodA</i>, <i>rpoS</i>, <i>cpxA</i>/<i>R</i>, <i>osmY</i>). This study provides a great understanding of the role of <i>ptsH</i> in diverse stress responses and virulence in <i>C. sakazakii</i>, and it contributes to our understanding of the genetic determinant of stress resistance and pathogenicity of this important foodborne pathogen.https://www.mdpi.com/2304-8158/11/17/2680<i>Cronobacter sakazakii</i><i>ptsH</i> genestress responseRNA-seq |
| spellingShingle | Yi Sun Jiahui Li Yanpeng Yang Gaoji Yang Yiqi Shi Shuo Wang Muxue Wang Xiaodong Xia The Role of <i>ptsH</i> in Stress Adaptation and Virulence in <i>Cronobacter sakazakii</i> BAA-894 Foods <i>Cronobacter sakazakii</i> <i>ptsH</i> gene stress response RNA-seq |
| title | The Role of <i>ptsH</i> in Stress Adaptation and Virulence in <i>Cronobacter sakazakii</i> BAA-894 |
| title_full | The Role of <i>ptsH</i> in Stress Adaptation and Virulence in <i>Cronobacter sakazakii</i> BAA-894 |
| title_fullStr | The Role of <i>ptsH</i> in Stress Adaptation and Virulence in <i>Cronobacter sakazakii</i> BAA-894 |
| title_full_unstemmed | The Role of <i>ptsH</i> in Stress Adaptation and Virulence in <i>Cronobacter sakazakii</i> BAA-894 |
| title_short | The Role of <i>ptsH</i> in Stress Adaptation and Virulence in <i>Cronobacter sakazakii</i> BAA-894 |
| title_sort | role of i ptsh i in stress adaptation and virulence in i cronobacter sakazakii i baa 894 |
| topic | <i>Cronobacter sakazakii</i> <i>ptsH</i> gene stress response RNA-seq |
| url | https://www.mdpi.com/2304-8158/11/17/2680 |
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