Heme Oxygenase-1 Is Involved in the Repair of Oxidative Damage Induced by Oxidized Fish Oil in <i>Litopenaeus vannamei</i> by Sulforaphane
Heme oxygenase-1 (HO-1), which could be highly induced under the stimulation of oxidative stress, functions in reducing the damage caused by oxidative stress, and sulforaphane (SFN) is an antioxidant. This study aims to investigate whether <i>HO-1</i> is involved in the repair of oxidati...
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MDPI AG
2023-10-01
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| Series: | Marine Drugs |
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| author | Junliang Luo Yongxiong Huang Yanghui Chen Yunhao Yuan Guojian Li Shuanghu Cai Jichang Jian Shiping Yang |
| author_facet | Junliang Luo Yongxiong Huang Yanghui Chen Yunhao Yuan Guojian Li Shuanghu Cai Jichang Jian Shiping Yang |
| author_sort | Junliang Luo |
| collection | DOAJ |
| description | Heme oxygenase-1 (HO-1), which could be highly induced under the stimulation of oxidative stress, functions in reducing the damage caused by oxidative stress, and sulforaphane (SFN) is an antioxidant. This study aims to investigate whether <i>HO-1</i> is involved in the repair of oxidative damage induced by oxidized fish oil (OFO) in <i>Litopenaeus vannamei</i> by sulforaphane (SFN). The oxidative stress model of <i>L. vannamei</i> was established by feeding OFO feed (OFO accounts for 6%), and they were divided into the following four groups: control group (injected with dsRNA-EGFP and fed with common feed), dsRNA-<i>HO-1</i> group (dsRNA-<i>HO-1</i>, common feed), dsRNA-<i>HO-1</i> + SFN group (dsRNA-<i>HO-1</i>, supplement 50 mg kg<sup>−1</sup> SFN feed), and SFN group (dsRNA-EGFP, supplement 50 mg kg<sup>−1</sup> SFN feed). The results showed that the expression level of <i>HO-1</i> in the dsRNA-<i>HO-1</i> + SFN group was significantly increased compared with the dsRNA-<i>HO-1</i> group (<i>p</i> < 0.05). The activities of SOD in muscle and GPX in hepatopancreas and serum of the dsRNA-<i>HO-1</i> group were significantly lower than those of the control group, and MDA content in the dsRNA-<i>HO-1</i> group was the highest among the four groups. However, SFN treatment increased the activities of GPX and SOD in hepatopancreas, muscle, and serum and significantly reduced the content of MDA (<i>p</i> < 0.05). SFN activated <i>HO-1</i>, upregulated the expression of antioxidant-related genes (<i>CAT</i>, <i>SOD</i>, <i>GST</i>, <i>GPX</i>, <i>Trx</i>, <i>HIF-1α</i>, <i>Nrf2</i>, <i>prx 2</i>, <i>Hsp 70</i>), and autophagy genes (<i>ATG 3</i>, <i>ATG 5</i>), and stabilized the expression of apoptosis genes (<i>caspase 2</i>, <i>caspase 3</i>) in the hepatopancreas (<i>p</i> < 0.05). In addition, knocking down <i>HO-1</i> aggravated the vacuolation of hepatopancreas and increased the apoptosis of hepatopancreas, while the supplement of SFN could repair the vacuolation of hepatopancreas and reduce the apoptosis signal. In summary, <i>HO-1</i> is involved in the repair of the oxidative damage induced by OFO in <i>L. vannamei</i> by SFN. |
| first_indexed | 2024-03-10T21:06:29Z |
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| series | Marine Drugs |
| spelling | doaj.art-ffb45bd14d044360b811f998ec6056dc2023-11-19T17:09:48ZengMDPI AGMarine Drugs1660-33972023-10-01211054810.3390/md21100548Heme Oxygenase-1 Is Involved in the Repair of Oxidative Damage Induced by Oxidized Fish Oil in <i>Litopenaeus vannamei</i> by SulforaphaneJunliang Luo0Yongxiong Huang1Yanghui Chen2Yunhao Yuan3Guojian Li4Shuanghu Cai5Jichang Jian6Shiping Yang7Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals, Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals, Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals, Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals, Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals, Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals, Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals, Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals, Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaHeme oxygenase-1 (HO-1), which could be highly induced under the stimulation of oxidative stress, functions in reducing the damage caused by oxidative stress, and sulforaphane (SFN) is an antioxidant. This study aims to investigate whether <i>HO-1</i> is involved in the repair of oxidative damage induced by oxidized fish oil (OFO) in <i>Litopenaeus vannamei</i> by sulforaphane (SFN). The oxidative stress model of <i>L. vannamei</i> was established by feeding OFO feed (OFO accounts for 6%), and they were divided into the following four groups: control group (injected with dsRNA-EGFP and fed with common feed), dsRNA-<i>HO-1</i> group (dsRNA-<i>HO-1</i>, common feed), dsRNA-<i>HO-1</i> + SFN group (dsRNA-<i>HO-1</i>, supplement 50 mg kg<sup>−1</sup> SFN feed), and SFN group (dsRNA-EGFP, supplement 50 mg kg<sup>−1</sup> SFN feed). The results showed that the expression level of <i>HO-1</i> in the dsRNA-<i>HO-1</i> + SFN group was significantly increased compared with the dsRNA-<i>HO-1</i> group (<i>p</i> < 0.05). The activities of SOD in muscle and GPX in hepatopancreas and serum of the dsRNA-<i>HO-1</i> group were significantly lower than those of the control group, and MDA content in the dsRNA-<i>HO-1</i> group was the highest among the four groups. However, SFN treatment increased the activities of GPX and SOD in hepatopancreas, muscle, and serum and significantly reduced the content of MDA (<i>p</i> < 0.05). SFN activated <i>HO-1</i>, upregulated the expression of antioxidant-related genes (<i>CAT</i>, <i>SOD</i>, <i>GST</i>, <i>GPX</i>, <i>Trx</i>, <i>HIF-1α</i>, <i>Nrf2</i>, <i>prx 2</i>, <i>Hsp 70</i>), and autophagy genes (<i>ATG 3</i>, <i>ATG 5</i>), and stabilized the expression of apoptosis genes (<i>caspase 2</i>, <i>caspase 3</i>) in the hepatopancreas (<i>p</i> < 0.05). In addition, knocking down <i>HO-1</i> aggravated the vacuolation of hepatopancreas and increased the apoptosis of hepatopancreas, while the supplement of SFN could repair the vacuolation of hepatopancreas and reduce the apoptosis signal. In summary, <i>HO-1</i> is involved in the repair of the oxidative damage induced by OFO in <i>L. vannamei</i> by SFN.https://www.mdpi.com/1660-3397/21/10/548<i>Litopenaeus vannamei</i>sulforaphane<i>HO-1</i>oxidative damageapoptosisautophagy |
| spellingShingle | Junliang Luo Yongxiong Huang Yanghui Chen Yunhao Yuan Guojian Li Shuanghu Cai Jichang Jian Shiping Yang Heme Oxygenase-1 Is Involved in the Repair of Oxidative Damage Induced by Oxidized Fish Oil in <i>Litopenaeus vannamei</i> by Sulforaphane Marine Drugs <i>Litopenaeus vannamei</i> sulforaphane <i>HO-1</i> oxidative damage apoptosis autophagy |
| title | Heme Oxygenase-1 Is Involved in the Repair of Oxidative Damage Induced by Oxidized Fish Oil in <i>Litopenaeus vannamei</i> by Sulforaphane |
| title_full | Heme Oxygenase-1 Is Involved in the Repair of Oxidative Damage Induced by Oxidized Fish Oil in <i>Litopenaeus vannamei</i> by Sulforaphane |
| title_fullStr | Heme Oxygenase-1 Is Involved in the Repair of Oxidative Damage Induced by Oxidized Fish Oil in <i>Litopenaeus vannamei</i> by Sulforaphane |
| title_full_unstemmed | Heme Oxygenase-1 Is Involved in the Repair of Oxidative Damage Induced by Oxidized Fish Oil in <i>Litopenaeus vannamei</i> by Sulforaphane |
| title_short | Heme Oxygenase-1 Is Involved in the Repair of Oxidative Damage Induced by Oxidized Fish Oil in <i>Litopenaeus vannamei</i> by Sulforaphane |
| title_sort | heme oxygenase 1 is involved in the repair of oxidative damage induced by oxidized fish oil in i litopenaeus vannamei i by sulforaphane |
| topic | <i>Litopenaeus vannamei</i> sulforaphane <i>HO-1</i> oxidative damage apoptosis autophagy |
| url | https://www.mdpi.com/1660-3397/21/10/548 |
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